LOCUS       Exported                1138 bp ds-DNA     linear   SYN 23-SEP-2019
DEFINITION  RH369_UBC9_C93A_2_T7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 23, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="color: #a6acb3"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /note="color: #cc99b2
                     Cleavage site after base 82"
                     /translation="LVPRGS"
     CDS             578..595
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     terminator      662..709
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="color: #ffffff"
     rep_origin      746..1127
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatgatgtcc ggaattgccc tctctcgctt
      121 agctcaggaa cgcaaagcgt ggcgcaaaga tcacccgttt gggtttgttg ccgtgcctac
      181 caaaaatcct gacggtacga tgaatcttat gaactgggaa tgcgcgatcc ctggcaagaa
      241 aggcacaccg tgggaaggcg gcttgttcaa gctgcgtatg ttgttcaaag atgattaccc
      301 gtcaagtccg cctaaatgta aattcgagcc gccgttattt catccaaacg tgtatcccag
      361 cggtacggtt gccctgagca tccttgaaga agacaaagat tggcgtcccg caattaccat
      421 caagcagatt ctgctcggca ttcaagagct gttaaatgaa ccaaacattc aggacccagc
      481 gcaggcagaa gcctatacta tctactgtca gaaccgtgta gagtatgaga agcgcgtccg
      541 tgcacaagcg aaaaagtttg ccccgtcgta actcgagcac caccaccacc accactgaga
      601 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      661 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      721 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
      781 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      841 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      901 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
      961 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     1021 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     1081 atctcggyct attcttttga tttataaggg aattttgccg atttcsggcc tattggta
//
