LOCUS       Exported                1076 bp ds-DNA     linear   SYN 24-SEP-2019
DEFINITION  170721_RH490-1_a_T7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 24, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             62..79
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /note="color: #cc99b2
                     Cleavage site after base 73"
                     /translation="LVPRGS"
     CDS             569..586
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     terminator      653..700
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="color: #ffffff"
ORIGIN
        1 aactttaaga aggagatata ccatgggcag cagccatcat catcatcatc acagcagcgg
       61 cctggtgccg cgcggcagcc atatgatgtc cggaattgcc ctctctcgct tagctcagga
      121 acgcaaagcg tggcgcaaag atcacccgtt tgggtttgtt gccgtgccta ccaaaaatcc
      181 tgacggtacg atgaatctta tgaactggga atgcgcgatc cctggcaaga aaggcacacc
      241 gtgggaaggc ggcttgttca agctgcgtat gttgttcaaa gatgattacc cgtcaagtcc
      301 gcctaaatgt aaattcgagc cgccgttatt tcatccaaac gtgtatccca gcggtacggt
      361 ttgcctgagc atccttgaag aagacaaaga ttggcgtccc gcaattacca tcaagcagat
      421 tctgctcggc attcaagagc tgttaaatga accaaacatt caggacccag cgcaggcaga
      481 agcctatact atctacgcgc agaaccgtgt agagtatgag aagcgcgtcc gtgcacaagc
      541 gaaaaagttt gccccgtcgt aactcgagca ccaccaccac caccactgag atccggctgc
      601 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      661 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      721 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgkggtggtt
      781 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      841 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      901 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      961 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     1021 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatcnc
//
