LOCUS       pCAG-roxPSTOProxP-Lyn-EGFP        6505 bp    DNA     circular
            
DEFINITION  pCAG-roxPSTOProxP-Lyn-EGFP
ACCESSION   pCAG-loxPSTOPloxP-ZsGreen	6500
VERSION     pCAG-loxPSTOPloxP-ZsGreen	6500.0
KEYWORDS    .

COMMENT
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                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
                    </line>
                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="199" y="215"/>
                <dimension height="412" width="412"/>
            </element>
            <element id="219665748" name="Lyn-EGFP"
                target="Lyn-EGFP:2644 - 3408" text="@N" type="21" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
                    </label>
                </style>
                <location x="453" y="672"/>
            </element>
            <element id="219665748" name="Lyn-EGFP"
                target="Lyn-EGFP:2644 - 3408" type="21" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="0" green="0" red="0"/>
                        <pattern style="1"/>
                    </line>
                </style>
            </element>
            <element id="219665748" name="Lyn-EGFP" shape="1"
                target="Lyn-EGFP:2644 - 3408" type="21" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
                    </line>
                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="224" y="240"/>
                <dimension height="362" width="362"/>
            </element>
            <element id="701666903" name="M13_pUC_fwd_primer"
                target="M13_pUC_fwd_primer:3757 - 3735" text="@N"
                type="21" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
                    </label>
                </style>
                <location x="195" y="648"/>
            </element>
            <element id="701666903" name="M13_pUC_fwd_primer"
                target="M13_pUC_fwd_primer:3757 - 3735" type="21" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="0" green="0" red="0"/>
                        <pattern style="1"/>
                    </line>
                </style>
            </element>
            <element id="701666903" name="M13_pUC_fwd_primer" shape="1"
                target="M13_pUC_fwd_primer:3757 - 3735" type="21" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
                    </line>
                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="174" y="190"/>
                <dimension height="462" width="462"/>
            </element>
            <element id="750074876" name="sv40_polyA"
                target="sv40_polyA:2430 - 2561" text="@N" type="21" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
                    </label>
                </style>
                <location x="575" y="612"/>
            </element>
            <element id="750074876" name="sv40_polyA"
                target="sv40_polyA:2430 - 2561" type="21" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="0" green="0" red="0"/>
                        <pattern style="1"/>
                    </line>
                </style>
            </element>
            <element id="750074876" name="sv40_polyA" shape="1"
                target="sv40_polyA:2430 - 2561" type="21" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
                    </line>
                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="199" y="215"/>
                <dimension height="412" width="412"/>
            </element>
            <element id="792492836" name="sv40_polyA"
                target="sv40_polyA:1922 - 2053" text="@N" type="21" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
                    </label>
                </style>
                <location x="645" y="508"/>
            </element>
            <element id="792492836" name="sv40_polyA"
                target="sv40_polyA:1922 - 2053" type="21" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="0" green="0" red="0"/>
                        <pattern style="1"/>
                    </line>
                </style>
            </element>
            <element id="792492836" name="sv40_polyA" shape="1"
                target="sv40_polyA:1922 - 2053" type="21" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
                    </line>
                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="199" y="215"/>
                <dimension height="412" width="412"/>
            </element>
            <element id="888980963" name="CAG_enhancer"
                target="CAG_enhancer:86 - 373" text="@N" type="21" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
                    </label>
                </style>
                <location x="456" y="160"/>
            </element>
            <element id="888980963" name="CAG_enhancer"
                target="CAG_enhancer:86 - 373" type="21" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="0" green="0" red="0"/>
                        <pattern style="1"/>
                    </line>
                </style>
            </element>
            <element id="888980963" name="CAG_enhancer" shape="1"
                target="CAG_enhancer:86 - 373" type="21" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
                    </line>
                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="199" y="215"/>
                <dimension height="412" width="412"/>
            </element>
            <element id="235773503" name="M13_pUC_rev_primer"
                target="M13_pUC_rev_primer:6456 - 6478" text="@N"
                type="21" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
                    </label>
                </style>
                <location x="405" y="55"/>
            </element>
            <element id="235773503" name="M13_pUC_rev_primer"
                target="M13_pUC_rev_primer:6456 - 6478" type="21" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="0" green="0" red="0"/>
                        <pattern style="1"/>
                    </line>
                </style>
            </element>
            <element id="235773503" name="M13_pUC_rev_primer" shape="1"
                target="M13_pUC_rev_primer:6456 - 6478" type="21" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
                    </line>
                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="224" y="240"/>
                <dimension height="362" width="362"/>
            </element>
            <element id="1936999851" name="bGH_PA_terminator"
                target="bGH_PA_terminator:3427 - 3654" text="@N"
                type="43" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
                    </label>
                </style>
                <location x="251" y="667"/>
            </element>
            <element id="1936999851" name="bGH_PA_terminator"
                target="bGH_PA_terminator:3427 - 3654" type="43" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="0" green="0" red="0"/>
                        <pattern style="1"/>
                    </line>
                </style>
            </element>
            <element id="1936999851" name="bGH_PA_terminator" shape="0"
                target="bGH_PA_terminator:3427 - 3654" type="43" xsi:type="symbol">
                <style name="Short Signal" shape="0" xsi:type="basicStyle">
                    <line width="3">
                        <color blue="204" green="102" red="51"/>
                        <pattern style="1"/>
                    </line>
                    <fill pattern="Solid">
                        <color blue="255" green="255" red="255"/>
                    </fill>
                </style>
                <location x="199" y="215"/>
                <dimension height="412" width="412"/>
            </element>
            <element name="Terminator" type="43" xsi:type="annotation">
                <label text="">
                    <style name="Signal Label" xsi:type="basicStyle">
                        <label text="@N">
                            <font name="Arial" size="9" style="1"/>
                            <color blue="0" green="0" red="0"/>
                        </label>
                        <line width="1">
                            <color blue="0" green="0" red="0"/>
                            <pattern style="1"/>
                        </line>
                    </style>
                </label>
                <connector>
                    <style name="Signal Label" xsi:type="basicStyle">
                        <label text="@N">
                            <font name="Arial" size="9" style="1"/>
                            <color blue="0" green="0" red="0"/>
                        </label>
                        <line width="1">
                            <color blue="0" green="0" red="0"/>
                            <pattern style="1"/>
                        </line>
                    </style>
                </connector>
                <symbol shape="0">
                    <style name="Short Signal" shape="13" xsi:type="basicStyle">
                        <line width="3">
                            <color blue="204" green="102" red="51"/>
                            <pattern style="1"/>
                        </line>
                        <fill pattern="Solid">
                            <color blue="255" green="255" red="255"/>
                        </fill>
                    </style>
                </symbol>
            </element>
            <element id="756108583" name="Membrane targeting motif"
                target="Membrane targeting motif:2644 - 2673" text="@N"
                type="87" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
                    </label>
                </style>
                <location x="568" y="679"/>
            </element>
            <element id="756108583" name="Membrane targeting motif"
                target="Membrane targeting motif:2644 - 2673" type="87" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="0" green="0" red="0"/>
                        <pattern style="1"/>
                    </line>
                </style>
            </element>
            <element id="756108583" name="Membrane targeting motif"
                shape="1" target="Membrane targeting motif:2644 - 2673"
                type="87" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
                    </line>
                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="199" y="215"/>
                <dimension height="412" width="412"/>
            </element>
        </elements>
    </setupInfo>
    <setupInfo complete="true" completeMinSize="150"
        completeNested="false" includeStopCodon="false"
        incomplete="false" incompleteMinSize="30" selected="false"
        startCodons="ATG GTG" stopCodons="TAA TGA TAG" type="ORFs"
        undefinedStart="false" undefinedStop="false" undefinedStopNested="false"/>
    <setupInfo allowBlunt="true" allowFivePrime="true"
        allowThreePrime="true"
        enzymeMap="AvaI|cycgrg,EcoRI|gaattc,SmaI|cccggg,PstI|ctgcag,ApaLI|gtgcac,NcoI|ccatgg,HindIII|aagctt,BamHI|ggatcc,XmaI|cccggg,ClaI|atcgat"
        enzymesCleavePoint="AvaI|2,EcoRI|2,SmaI|4,PstI|6,ApaLI|2,NcoI|2,HindIII|2,BamHI|2,XmaI|2,ClaI|3"
        enzymesComplCleavePoint="AvaI|0,EcoRI|0,SmaI|0,PstI|0,ApaLI|0,NcoI|0,HindIII|0,BamHI|0,XmaI|0,ClaI|0"
        enzymesPalindromic="AvaI|true,EcoRI|true,SmaI|true,PstI|true,ApaLI|true,NcoI|true,HindIII|true,BamHI|true,XmaI|true,ClaI|true"
        ignoreIn="-1,-1" ignoreLess="-1" ignoreMore="-1"
        ignoreOut="-1,-1" selected="true" startIndex="1" type="Restriction Map"/>
    <setupInfo featureList="" selected="true" type="Feature Map"/>
    <setupInfo basesPerBlock="10" blocksPerLine="10"
        prefer1LetterAACode="false" preferSingleStrand="false"
        selected="true" textBold="false" textColor="0,0,0"
        textFontName="Andale Mono" textFontSize="14" textItalic="false"
        textUnderline="false" trackSeparatorColor="128,128,128"
        trackSeparatorWidth="2" type="Sequence"
        xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:type="sequenceSetup">
        <textRangeSetupList/>
    </setupInfo>
    <setupInfo analysisSubsets="Analysis Results (MAIN)" selected="true" type="Auto Load Analysis"/>
</vntiDisplaySetup>
DISPLAY_SETUP_END|
COMMENT     Backbone is pCAG-loxPSTOPloxP-Lyn-EGFP
            
            pCAG-loxPSTOPloxP-Lyn-EGFP was diggested with SalI/PstI
            
            roxP-STOP-roxP was amplified from pCAG-roxPSTOProxP-ZsGreen and
            cloned into SalI/PstI
            
            sites by LIC cloning.
            
            Resistance= Ampicilin
            VNTEXTCHREPL|Animal/Other Eukaryotic
            VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     misc_feature    86..373
                     /label=CAG_enhancer
     CDS             complement(805..1536)
                     /label=ORF frame 3
                     /translation="MEKGTAARRRTKALPAHFLPAGAAPLRPRPLEGVRRRLPDFGSA
                     RFGTKEVPAPSRTITIKGNGCGSPRLDSRRRSGAAAPLPRALPGPRRPRPARHPHLPP
                     PPARHGEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLC
                     SPRSPPAPRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSR
                     SPPHKGPSRSPLRLSRSHRKETSRH*"
     CDS             complement(846..1349)
                     /label=ORF frame 1
                     /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPG
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT
                     KGPPGAL*"
     promoter        1686..1704
                     /label=T7_promoter
                     /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPG
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT
                     KGPPGAL*"
     misc_feature    complement(3424..3441)
                     /label=BGH_rev_primer
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALP*"
     terminator      3427..3654
                     /label=bGH_PA_terminator
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALP*"
     CDS             3628..4101
                     /label=ORF frame 2
                     /translation="MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQRRDWE
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        complement(3726..3742)
                     /label=M13_forward20_primer
                     /translation="MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQRRDWE
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(3735..3757)
                     /label=M13_pUC_fwd_primer
                     /translation="MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQRRDWE
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    3735..3883
                     /label=lacZ_a
                     /translation="MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQRRDWE
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     rep_origin      3901..4207
                     /label=f1_origin
                     /translation="MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQRRDWE
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        4400..4428
                     /label=AmpR_promoter
                     /translation="MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQRRDWE
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     gene            4470..5330
                     /label=Ampicillin
                     /gene="Ampicillin"
                     /translation="MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQRRDWE
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     CDS             4470..5330
                     /label=ORF frame 1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5485..6104
                     /label=pBR322_origin
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        6413..6442
                     /label=lac_promoter
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    6456..6478
                     /label=M13_pUC_rev_primer
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        6477..6495
                     /label=M13_reverse_primer
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     eukaryotic      86..1536
                     /label=CAG promoter
                     /promoter_type="eukaryotic"
                     /type="eukaryotic"
                     /vntifkey="29"
     misc_signal     2644..2673
                     /label=Membrane targeting motif
                     /note="MGCIKSKRKD peptide from Lym kinase"
                     /type="misc_signal"
                     /vntifkey="87"
     misc_marker     2689..3408
                     /label=EGFP
                     /type="misc_marker"
                     /vntifkey="22"
     misc_feature    2644..3408
                     /label=Lyn-EGFP
                     /note="EGFP tagged with the membrane target domain of
                     Lyn-Kinase"
                     /type="misc_feature"
                     /vntifkey="21"
     misc_structure  2590..2621
                     /label=Rox recombination site
                     /type="misc_structure"
                     /vntifkey="88"
     misc_feature    2176..2307
                     /label=sv40_polyA
                     /note="short sv40_polyA"
                     /type="misc_feature"
                     /vntifkey="21"
     misc_structure  1721..1752
                     /label=Rox recombination site
                     /type="misc_structure"
                     /vntifkey="88"
     misc_feature    2430..2561
                     /label=sv40_polyA
                     /note="short sv40_polyA"
                     /type="misc_feature"
                     /vntifkey="21"
     misc_feature    1922..2053
                     /label=sv40_polyA
                     /note="short sv40_polyA"
                     /type="misc_feature"
                     /vntifkey="21"
     misc_structure  1922..2561
                     /label=STOP
                     /type="misc_structure"
                     /vntifkey="88"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
      541 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
      841 aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagatcctta
     1681 attaataata cgactcacta tagggggtcg acccgccacc taactttaaa taattggcat
     1741 tatttaaagt tatcgcgatg aataaatgaa agcttgcaga tctgcgactc tagaggatct
     1801 gcgactctag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     1861 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     1921 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1981 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     2041 tcatgtctgg atctgcgact ctagaggatc ataatcagcc ataccacatt tgtagaggtt
     2101 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     2161 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     2221 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     2281 atcaatgtat cttatcatgt ctggatctgc gactctagag gatcataatc agccatacca
     2341 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     2401 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     2461 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2521 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccccatcaag ctgatccgga
     2581 acccttaatt aactttaaat aattggcatt atttaaagtt aggcagccgc cctgcaggcc
     2641 accatgggat gtattaaatc aaaaaggaaa gacggggccc ggtaccatat ggtgagcaag
     2701 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     2761 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     2821 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     2881 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     2941 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     3001 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     3061 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     3121 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     3181 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     3241 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     3301 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3361 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaga gctcgctgat
     3421 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     3481 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     3541 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     3601 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     3661 aggcggaaag aaccagctgg ggctcgagat ccactagttc tagcctcgag gctagagcgg
     3721 ccgccactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     3781 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     3841 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     3901 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3961 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4021 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4081 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4141 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4201 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4261 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4321 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4381 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4441 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4501 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4561 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4621 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4681 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4741 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4801 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4861 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4921 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4981 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5041 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5101 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5161 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5221 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5281 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5341 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5401 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5461 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5521 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5581 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5641 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5701 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5761 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5821 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5881 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5941 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6001 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6061 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6121 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6181 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6241 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6301 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6361 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     6421 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     6481 acagctatga ccatgaggcg cgccg
//
