LOCUS       pTub-alpha1-HA-Dre        5190 bp    DNA     circular
            21-SEP-2017
DEFINITION  
ACCESSION   DNA_pCAGIG
VERSION     DNA_pCAGIG.0
KEYWORDS    .

COMMENT
DISPLAY_SETUP_START|
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                <dimension height="312" width="312"/>
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                <location x="54" y="494"/>
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            <element id="75629578" name="Tub-alpha1\promoter"
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                    </fill>
                </style>
                <location x="113" y="130"/>
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            <element id="1195781551" target="1195781551" text="@L bp"
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                </style>
                <location x="264" y="315"/>
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            <element id="1181576869" name="pBR322_origin"
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                <location x="5" y="354"/>
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                <location x="113" y="130"/>
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            <element id="1899176867" name="ampicilin\promoter"
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                <location x="294" y="70"/>
            </element>
            <element id="1899176867" name="ampicilin\promoter"
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                        <color blue="0" green="0" red="0"/>
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            </element>
            <element id="1899176867" name="ampicilin\promoter" shape="1"
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                <style name="Shape 2" shape="1" xsi:type="basicStyle">
                    <line width="1">
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                </style>
                <location x="113" y="130"/>
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            <element id="1060553105" target="1060553105" text="@N"
                type="346519525" xsi:type="label">
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                    <label text="@N">
                        <font name="Arial" size="24" style="1"/>
                        <color blue="50" green="50" red="50"/>
                    </label>
                </style>
                <location x="178" y="287"/>
            </element>
            <element id="846382515" name="Ampicilin\Resistance"
                target="Ampicilin\Resistance:5059 - 4199" text="@N"
                type="21" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
                    </label>
                </style>
                <location x="59" y="120"/>
            </element>
            <element id="846382515" name="Ampicilin\Resistance"
                target="Ampicilin\Resistance:5059 - 4199" type="21" xsi:type="line">
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                shape="1" target="Ampicilin\Resistance:5059 - 4199"
                type="21" xsi:type="symbol">
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                    <fill pattern="Solid">
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                    </fill>
                </style>
                <location x="113" y="130"/>
                <dimension height="362" width="362"/>
            </element>
            <element id="189129316" name="NLS-HA-Dre"
                target="NLS-HA-Dre:1125 - 2207" text="@N" type="21" xsi:type="label">
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                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
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                    </label>
                </style>
                <location x="502" y="410"/>
            </element>
            <element id="189129316" name="NLS-HA-Dre"
                target="NLS-HA-Dre:1125 - 2207" type="21" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
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                        <color blue="0" green="0" red="0"/>
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            </element>
            <element id="189129316" name="NLS-HA-Dre" shape="1"
                target="NLS-HA-Dre:1125 - 2207" type="21" xsi:type="symbol">
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                </style>
                <location x="113" y="130"/>
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            <element id="2145867337" name="sv40_origin"
                target="sv40_origin:2912 - 2989" text="@N" type="87" xsi:type="label">
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                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
                    </label>
                </style>
                <location x="126" y="566"/>
            </element>
            <element id="2145867337" name="sv40_origin"
                target="sv40_origin:2912 - 2989" type="87" xsi:type="line">
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                <style name="Shape 1" shape="1" xsi:type="basicStyle">
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                <dimension height="412" width="412"/>
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        </elements>
    </setupInfo>
    <setupInfo complete="true" completeMinSize="150"
        completeNested="false" includeStopCodon="false"
        incomplete="false" incompleteMinSize="30" selected="false"
        startCodons="ATG GTG" stopCodons="TAA TGA TAG" type="ORFs"
        undefinedStart="false" undefinedStop="false" undefinedStopNested="false"/>
    <setupInfo allowBlunt="true" allowFivePrime="true"
        allowThreePrime="true"
        enzymeMap="AvaI|cycgrg,EcoRI|gaattc,SmaI|cccggg,PstI|ctgcag,ApaLI|gtgcac,NcoI|ccatgg,HindIII|aagctt,BamHI|ggatcc,XmaI|cccggg,ClaI|atcgat"
        enzymesCleavePoint="AvaI|2,EcoRI|2,SmaI|4,PstI|6,ApaLI|2,NcoI|2,HindIII|2,BamHI|2,XmaI|2,ClaI|3"
        enzymesComplCleavePoint="AvaI|0,EcoRI|0,SmaI|0,PstI|0,ApaLI|0,NcoI|0,HindIII|0,BamHI|0,XmaI|0,ClaI|0"
        enzymesPalindromic="AvaI|true,EcoRI|true,SmaI|true,PstI|true,ApaLI|true,NcoI|true,HindIII|true,BamHI|true,XmaI|true,ClaI|true"
        ignoreIn="-1,-1" ignoreLess="-1" ignoreMore="-1"
        ignoreOut="-1,-1" selected="true" startIndex="1" type="Restriction Map"/>
    <setupInfo featureList="" selected="true" type="Feature Map"/>
    <setupInfo basesPerBlock="10" blocksPerLine="10"
        prefer1LetterAACode="false" preferSingleStrand="false"
        selected="true" textBold="false" textColor="0,0,0"
        textFontName="Andale Mono" textFontSize="14" textItalic="false"
        textUnderline="false" trackSeparatorColor="128,128,128"
        trackSeparatorWidth="2" type="Sequence"
        xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:type="sequenceSetup">
        <textRangeSetupList>
            <textRangeSetup bold="false" fontName="" fontSize="0"
                italic="false" startIndex="84" stopIndex="371" underline="false"/>
        </textRangeSetupList>
    </setupInfo>
    <setupInfo analysisSubsets="Analysis Results (MAIN)" selected="true" type="Auto Load Analysis"/>
</vntiDisplaySetup>
DISPLAY_SETUP_END|
COMMENT     Backbone if pTa1-Cre-myc (originated from pCAG-Cre-myc Addgene
            Plasmid #13775).
            
            pTa1-Cre-myc was digested with EcoRI and BglII excising the Cre-myc
            
            NLS-HA-Dre was amplified from  pCAG-NLS-HA-Dre ( Addgene Plasmid
            #51272)
             With primers:
            LIC-Ta1-Dre Rv 5’- ATTTTTGGCAGAGGGAAAAAGATCTGACTCAGCTATCCATCAGTC-3’
            LIC-Ta1-Dre Fw 5’-CAGCCGCGAAGCAGCAACGAATTCGCCACCATGCCTAAGAAGAAG-3’
             (1100 bp):
            
            Fragment was LIC clones into the above vector resulting in
            pTa1-NLS-HA-Dre LIC cloning.
            
            Resistance ampicilin
            This file is created by Vector NTI
            ORIGDB|GenBank
            VNTDBDATE|
            VNTEXTCHREPL|Animal/Other Eukaryotic
            VNTREPLTYPE|Plasmid
            VNTSEQSTYLE|84|371||0|null|null|null|null|39,48,239
            This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     polyA_signal    2221..2647
                     /type="polyA_signal"
                     /vntifkey="25"
                     /label=rb_globin_polyA_terminator
     promoter        2877..3056
                     /promoter_type="eukaryotic"
                     /type="eukaryotic"
                     /vntifkey="29"
                     /label=sv40_promoter
                     /note="Unknown feature type:eukaryotic"
     misc_signal     2912..2989
                     /type="misc_signal"
                     /vntifkey="87"
                     /label=sv40_origin
     polyA_signal    3070..3201
                     /type="polyA_signal"
                     /vntifkey="25"
                     /label=sv40_polyA_terminator
     oriT            3425..4044
                     /type="oriT"
                     /vntifkey="104"
                     /label=pBR322_origin
     misc_feature    complement(4199..5059)
                     /type="misc_feature"
                     /vntifkey="21"
                     /label=Ampicilin\Resistance
     promoter        complement(5101..5129)
                     /type="promoter"
                     /vntifkey="30"
                     /label=ampicilin\promoter
     promoter        25..1112
                     /promoter_type="eukaryotic"
                     /type="eukaryotic"
                     /vntifkey="29"
                     /label=Tub-alpha1\promoter
                     /note="Unknown feature type:eukaryotic  Neuron specific
                     promoter"
     misc_structure  1134..1148
                     /type="misc_structure"
                     /vntifkey="88"
                     /label=NLS
     misc_feature    1125..2207
                     /type="misc_feature"
                     /vntifkey="21"
                     /label=NLS-HA-Dre
                     /note="DreO recombinase"
     misc_marker     1149..1175
                     /type="misc_marker"
                     /vntifkey="22"
                     /label=HA\tag
     misc_structure  1128..1148
                     /type="misc_structure"
                     /vntifkey="88"
                     /label=SV40_NLS
ORIGIN
        1 gtcgacattg attattgact agtcaattcc gtattaggag ggatggctca tttctaggga
       61 caaaataaca caggctctgg ggtgaggtag ggtcgggtaa gggggcgagc ggggaggggt
      121 gatgggagag cactaccgct aaagaaagca agctctctag taaagcgtta agtactactt
      181 tgtatattgt ttctcttttt tcctttctct ctctctctct ctttgtttgt ttttggtttt
      241 tgagacagga tatcacacta tatatccagg ccgggtacaa aataaccgca gtcctccgct
      301 tcccaatgct gggatttaca ggcataaacc actaagggcg gttatgatga ccttgagctg
      361 caagtcttcc tgcctctgcc tcccgggtgc tgttgagggt cataggcgtg gtctattcat
      421 actgagcttc tgaattttgc tcaaatatta ataataatag taataataat aaccacaata
      481 atacaactgt aaaactaaac atttacccac gcctttttga ccatcattcc catagctctt
      541 gctactttat ttaaagcgaa cagagatgtt gaatcctgac ggaacgtatt taaatttagt
      601 gtagtataaa tgaaaagctg gaatttacca taaagaatct caacacaaat tctgtgatta
      661 agtgttgggg aaaaccttaa attatcctaa ctacagttta aggtttaagc tccgtataat
      721 cacccaaccc ccgttttctt tcttccctct ctacccctcc ccagctccac cccataatgg
      781 atgctcggct agttgctttt gcgaggcctt tgtctgaagg atgcaaaatc tacggatgct
      841 agcgaggggg ggaagggggg agagattacc tcataccatg tcgcttgcac caatcaccac
      901 tctcctgtcg cggcttctct gggcagacgg aggggtctgg accaacagga aaaggccttg
      961 gcccatcccc atggtgaccg agctgtatat aaggaggcgc atccgcccaa gtgccgcagg
     1021 ttctcttaca tcgaccgctt aagagtcgcg ctgtaagaag caacacctcc tcctcgcctc
     1081 cgccatccac ccggcagccg cgaagcagca acgaattcgc caccatgcct aagaagaaga
     1141 ggaaggttta cccatacgat gttccagatt acgctggatc ctctgagctg attattagtg
     1201 gttcatctgg tggattcctg cgaaacatcg gcaaagagta tcaggaggcc gctgaaaact
     1261 tcatgaggtt tatgaatgac cagggggcgt acgctcctaa cactttgagg gatttgaggt
     1321 tggtctttca tagctgggcc agatggtgcc atgctcggca gcttgcatgg tttccaatta
     1381 gtcctgaaat ggcacgcgaa tactttcttc agttgcacga tgcagacctg gcctccacta
     1441 ccatcgacaa gcactatgct atgcttaata tgcttctgtc ccactgcgga ctgccaccct
     1501 tgtccgacga caagtcagtg agtcttgcca tgagaagaat tagaagagaa gccgcaaccg
     1561 aaaagggtga gaggacagga caggcaatcc ccctgcgctg ggacgacctg aagctgctgg
     1621 atgtgctgct cagcaggagc gagcggctgg tcgacctgcg caacagggct ttcctgttcg
     1681 tagcctataa caccctcatg agaatgtctg aaatatcacg catcagggtt ggggacttgg
     1741 atcagacagg agacacagtg accctgcaca tcagtcacac taagacaatc accacagctg
     1801 cgggccttga caaagtgctc tcccggcgaa ccacagcagt gctcaatgac tggctggacg
     1861 tcagtgggct tagagaacat ccagacgctg tgctcttccc acctatacac cggtcaaaca
     1921 aagcccgcat tactaccacg cccctgaccg cccctgccat ggagaagatt ttcagtgatg
     1981 cctgggtgct gctgaacaaa cgggacgcca cccccaataa agggaggtat aggacctgga
     2041 ccggccattc cgccagggtg ggtgccgcaa tagacatggc cgagaaacag gtgtctatgg
     2101 tcgagattat gcaggaaggg acatggaaga agcctgaaac actgatgcgg tatctcagaa
     2161 ggggcggagt gtccgtggga gccaattctc gactgatgga tagctgagtc agatcttttt
     2221 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     2281 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     2341 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     2401 gcaacatatg ccatatgctg gctgccatga acaaaggtgg ctataaagag gtcatcagta
     2461 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     2521 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     2581 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     2641 tcttctctta tgaagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc
     2701 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2761 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2821 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
     2881 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     2941 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3001 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3061 aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3121 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3181 gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3601 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     3661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3841 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     3901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4141 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4201 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4261 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4321 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4381 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4441 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4501 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4561 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4621 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4681 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4741 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4801 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4861 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4921 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4981 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5041 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5101 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5161 cgcgcacatt tccccgaaaa gtgccacctg
//
