LOCUS       Exported                4097 bp ds-DNA     circular SYN 20-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   DNA_pFN22K-mCMVd1-EGFP-RhoA(WT)
VERSION     .
KEYWORDS    pFN22K-mCMVd1-EGFP-RhoA(WT)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 24, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
COMMENT     Backbone source:
                 pFN22K HaloTag*CMVd1Flexi(R)Vector (Promega).
                 Digested in HindIII/SalI
                 EGFP-RhoA (WT) (HindIII/XhoI) from Addgene (Plasmid #12965)
                 VNTEXTCHREPL|Animal/Other Eukaryotic
                 VNTREPLTYPE|Plasmid
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..120
                     /label=CMVd1 promoter
                     /label=CMVd1\promoter
                     /note="Unknown feature type:eukaryotic Deletion of the CMV 
                     oroter. It drives lower levelsof expression than the intact
                     CMV promoter"
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    139..861
                     /label=EGFP
                     /label=nonstandard type: misc_marker
                     /note="Dominant negative"
                     /note="/type=misc_marker"
                     /note="/vntifkey=22"
     mat_peptide     142..1443
                     /label=EGFP-hRhoA (WT)
                     /label=EGFP-hRhoA\(WT)
                     /note="/type=mat_peptide"
                     /note="/vntifkey=84"
     misc_structure  862..1443
                     /label=hRhoA (WT)
                     /label=hRhoA\(WT)
                     /note="/type=misc_structure"
                     /note="/vntifkey=88"
     polyA_signal    1562..1782
                     /label=sv40_polyA_late signal
                     /label=sv40_polyA_late\signal
                     /note="/type=polyA_signal"
                     /note="/vntifkey=25"
     misc_structure  2091..2885
                     /label=Kanamycin resistance gene
                     /label=Kanamycin\resistance\gene
                     /note="/type=misc_structure"
                     /note="/vntifkey=88"
     oriT            3054..3090
                     /direction=RIGHT
                     /label=Replication origen
                     /label=Replication\origen
                     /note="/type=oriT"
                     /note="/vntifkey=104"
     misc_feature    3761..4046
                     /label=Cer Region
                     /label=Cer\Region
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
ORIGIN
        1 ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg
       61 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
      121 cactagaagc ttgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      181 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      241 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      301 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
      361 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      421 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      481 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      541 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      601 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
      661 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      721 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
      781 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      841 gacgagctgt acaaggaatt catggctgcc atccggaaga aactggtgat tgttggtgat
      901 ggagcctgtg gaaagacatg cttgctcata gtcttcagca aggaccagtt cccagaggtg
      961 tatgtgccca cagtgtttga gaactatgtg gcagatatcg aggtggatgg aaagcaggta
     1021 gagttggctt tgtgggacac agctgggcag gaagattatg atcgcctgag gcccctctcc
     1081 tacccagata ccgatgttat actgatgtgt ttttccatcg acagccctga tagtttagaa
     1141 aacatcccag aaaagtggac cccagaagtc aagcatttct gtcccaacgt gcccatcatc
     1201 ctggttggga ataagaagga tcttcggaat gatgagcaca caaggcggga gctagccaag
     1261 atgaagcagg agccggtgaa acctgaagaa ggcagagata tggcaaacag gattggcgct
     1321 tttgggtaca tggagtgttc agcaaagacc aaagatggag tgagagaggt ttttgaaatg
     1381 gctacgagag ctgctctgca agctagacgt gggaagaaaa aatctggttg ccttgtcttg
     1441 tgactcgacc tgcaggcatg caagctgatc cggctgctaa caaagcccga aaggaagctg
     1501 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcg gccgcttcga
     1561 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     1621 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     1681 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     1741 tgggaggttt ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatcga attctaatgg
     1801 atcctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg
     1861 ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg
     1921 cgccctgagt gcttgcggca gcgtgagctt caaaagaatt gccagctggg gcgccctctg
     1981 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
     2041 ggcgcagggg atcaagatct gatcaagaga caggatgacg gtcgtttcgc atgcttgaac
     2101 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     2161 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     2221 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     2281 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     2341 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     2401 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     2461 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     2521 cacgcactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     2581 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg tatgccggat ggtgaggatc
     2641 tcgtcgtgac tcatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     2701 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     2761 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     2821 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     2881 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc ggtatcagct
     2941 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3001 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3061 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3481 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ttcaagaaga tcctttgatc
     3661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3721 agattatcaa aaaggatctt cacctagatc cttttatagt ccggaaatac aggaacgcac
     3781 gctggatggc ccttcgctgg gatggtgaaa ccatgaaaaa tggcagcttc agtggattaa
     3841 gtgggggtaa tgtggcctgt accctctggt tgcataggta ttcatacggt taaaatttat
     3901 caggcgcgat tgcggcagtt tttcgggtgg tttgttgcca tttttacctg tctgctgccg
     3961 tgatcgcgct gaacgcgttt tagcggtgcg tacaattaag ggattatggt aaatccactt
     4021 actgtctgcc ctcgtagcca tcgagataaa ccgcagtact ccggccacga tgcgtccggc
     4081 gtagaggatc gagatct
//
