LOCUS       Exported                5244 bp ds-DNA     circular SYN 24-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   DNA_pEGFP-N1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 24, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
COMMENT     Backbone: pTub-alpha1-ZsGreen-N1. Original backbone pCMV-ZsGreen-N1 
            from clontech.
            pTub-alpha1-ZsGreen-N1 (PstI/NotI)
            
            Insert EGFP (PstI/NotI) from pEGFP-N2
            
            Regular ligation
            
            Resistance kanamycin
            
            VNTEXTCHREPL|Animal/Other Eukaryotic
            VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    6..1102
                     /label=Tub-alpha-1
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_structure  1103..1140
                     /label=MCS
                     /note="/type=misc_structure"
                     /note="/vntifkey=88"
     exon            1190..1909
                     /label=EGFP
                     /note="/type=exon"
                     /note="/vntifkey=61"
     polyA_signal    1928..2153
                     /label=SV40_PA
                     /note="/type=polyA_signal"
                     /note="/vntifkey=25"
     oriT            2292..2598
                     /label=f1_origin
                     /note="/type=oriT"
                     /note="/vntifkey=104"
     misc_feature    2789..3057
                     /label=sv40
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     gene            3143..3931
                     /label=NeoR/KanR
                     /note="Resistance gene"
                     /note="/type=gene"
                     /note="/vntifkey=60"
     polyA_site      4109..4378
                     /label=TK_PA_Terminator
                     /note="/type=polyA_site"
                     /note="/vntifkey=26"
     oriT            4526..5145
                     /label=pB322_origen
                     /note="/type=oriT"
                     /note="/vntifkey=104"
ORIGIN
        1 tagttattat gctgaattcc gtattaggag ggatggctca tttctaggga caaaataaca
       61 caggctctgg ggtgaggtag ggtcgggtaa gggggcgagc ggggaggggt gatgggagag
      121 cactaccgct aaagaaagca agctctctag taaagcgtta agtactactt tgtatattgt
      181 ttctcttttt tcctttctct ctctctctct ctttgtttgt ttttggtttt tgagacagga
      241 tatcacacta tatatccagg ccgggtacaa aataaccgca gtcctccgct tcccaatgct
      301 gggatttaca ggcataaacc actaagggcg gttatgatga ccttgagctg caagtcttcc
      361 tgcctctgcc tcccgggtgc tgttgagggt cataggcgtg gtctattcat actgagcttc
      421 tgaattttgc tcaaatatta ataataatag taataataat aaccacaata atacaactgt
      481 aaaactaaac atttacccac gcctttttga ccatcattcc catagctctt gctactttat
      541 ttaaagcgaa cagagatgtt gaatcctgac ggaacgtatt taaatttagt gtagtataaa
      601 tgaaaagctg gaatttacca taaagaatct caacacaaat tctgtgatta agtgttgggg
      661 aaaaccttaa attatcctaa ctacagttta aggtttaagc tccgtataat cacccaaccc
      721 ccgttttctt tcttccctct ctacccctcc ccagctccac cccataatgg atgctcggct
      781 agttgctttt gcgaggcctt tgtctgaagg atgcaaaatc tacggatgct agcgaggggg
      841 ggaagggggg agagattacc tcataccatg tcgcttgcac caatcaccac tctcctgtcg
      901 cggcttctct gggcagacgg aggggtctgg accaacagga aaaggccttg gcccatcccc
      961 atggtgaccg agctgtatat aaggaggcgc atccgcccaa gtgccgcagg ttctcttaca
     1021 tcgaccgctt aagagtcgcg ctgtaagaag caacacctcc tcctcgcctc cgccatccac
     1081 ccggcagccg cgaagcagca acaccggtga gcgggggatc tcgagctcaa gcttcgaatt
     1141 ctgcagtcga cggtaccgcg ggcccgggat ccaccggccg gtcgccacca tggtgagcaa
     1201 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     1261 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     1321 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     1381 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     1441 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     1501 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     1561 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     1621 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     1681 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     1741 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     1801 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     1861 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga
     1921 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     1981 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     2041 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2101 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta
     2161 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     2221 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     2281 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     2341 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     2401 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     2461 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     2521 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     2581 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     2641 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2701 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     2761 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     2821 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     2881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     2941 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3001 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3061 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     3121 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     3181 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     3241 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     3301 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     3361 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     3421 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     3481 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     3541 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     3601 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     3661 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     3721 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     3781 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     3841 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     3901 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     3961 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     4021 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     4081 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     4141 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     4201 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     4261 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     4321 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     4381 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4441 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4501 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4561 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4621 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4681 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4741 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4801 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4861 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4921 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4981 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5041 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5101 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5161 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5221 gataaccgta ttaccgccat gcat
//
