LOCUS       pLV-Hyg-Snrnp40-       10123 bp    DNA     circular     26-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     
            This file is created by Biosettia Inc
            http://www.biosettia.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007770|
COMMENT     VNTDBDATE|-13007770|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLV-Hyg-Snrnp40-skywarp-CRISPR-resistant|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     CDS             8044..8904
                     /vntifkey="4"
                     /label=Amp(R)
     promoter        9105..9645
                     /vntifkey="30"
                     /label=pUC\ori
     promoter        7..235
                     /vntifkey="30"
                     /label=RSV
     LTR             6649..6883
                     /vntifkey="19"
                     /label=3'\LTR
     intron          526..1690
                     /vntifkey="15"
                     /label=Intron
     rep_origin      complement(7458..7913)
                     /vntifkey="33"
                     /label=f1\ori
     LTR             236..416
                     /vntifkey="19"
                     /label=5'LTR
     misc_feature    7086..7299
                     /vntifkey="21"
                     /label=SV40\ori
     misc_feature    5970..6566
                     /vntifkey="21"
                     /label=WPRE
     promoter        1933..2327
                     /vntifkey="30"
                     /label=human\EF1a\promoter
     intron          2361..3303
                     /vntifkey="15"
                     /label=intron
     CDS             4935..5960
                     /vntifkey="4"
                     /label=Hyg
     RBS             4327..4911
                     /vntifkey="32"
                     /label=IRES
     misc_feature    3329..4282
                     /vntifkey="21"
                     /label=Snrnp40-skywarp-CRISPR-resistant
BASE COUNT     2432 a      2531 c      2726 g      2434 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagaat gatcgagcag cagaagcgta agggcccgga 
     3361 gctacccctg gttcccgtca agcggccgcg acatgagttg ctgttgggag cggcaggagc 
     3421 cggccccgga gcggggccgc agcaggcgac tccaggggca ttgctgcagg cgggaccgcc 
     3481 caggtgttct tcccttcaag ctccaatcat gctgctctcg ggacatgagg gggaagtgta 
     3541 ctgctgcaag tttcacccca atggatccac cctggcttct gcaggatttg accggctaat 
     3601 actgttgtgg aacgtctatg gagactgtga caattacgcc accttaaagg gacacagtgg 
     3661 agcagtgatg gagctgcact ataacacaga tggcagcatg ctcttctcag cgtcgacaga 
     3721 caaaactgtg gcggtgtggg atagtgaaac tggggagaga gtgaaaaggc taaaggggca 
     3781 tacttccttt gtgaactcct gttacccagc caggcggggc ccccaacttg tctgtactgg 
     3841 cagtgacgat ggtacagtta aggtctggga cctacgccaa aacaagttaa cctacaccat 
     3901 gagaggccat gcagactctg taactggcct gagcttgagt tctgaaggct cttatctcct 
     3961 gtccaatgca atggacaaca cagttcgtgt ctgggatgtc cggccatttg ctcccaaaga 
     4021 gagatgtgtg aagatatttc aaggaaatgt ccacaacttt gaaaagaacc tcctgagatg 
     4081 ttcctggtct ccagatggaa gcaagatagc agctggctca gcagacaggt ttgtgtacgt 
     4141 gtgggacacc acaagccgaa gggtcctgta caagctgcct ggtcacgctg gctccatcaa 
     4201 tgaagtggct ttccaccccg acgagcccat catcctctca gcatccagtg acaagaggct 
     4261 gtacatgggt gagatccagt gaggatccac gcgtctccgg cctagggata acagggtaat 
     4321 ccgctagccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag 
     4381 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga 
     4441 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg 
     4501 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt 
     4561 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca 
     4621 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc 
     4681 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat 
     4741 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc 
     4801 ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga 
     4861 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac actagagatc 
     4921 caccggtcgc caccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga 
     4981 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg 
     5041 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg 
     5101 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg 
     5161 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac 
     5221 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg 
     5281 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat 
     5341 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg 
     5401 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc 
     5461 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc 
     5521 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg 
     5581 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga 
     5641 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc 
     5701 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc 
     5761 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa 
     5821 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg 
     5881 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc 
     5941 gtccgagggc aaaggaatag gcggccgcgt cgacaatcaa cctctggatt acaaaatttg 
     6001 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 
     6061 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 
     6121 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 
     6181 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 
     6241 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 
     6301 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 
     6361 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 
     6421 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 
     6481 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 
     6541 ctccctttgg gccgcctccc cgcctggaat tcgagctcgg tacctttaag accaatgact 
     6601 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 
     6661 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac 
     6721 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 
     6781 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 
     6841 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 
     6901 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg 
     6961 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 
     7021 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 
     7081 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 
     7141 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 
     7201 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt 
     7261 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg 
     7321 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt 
     7381 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag 
     7441 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 
     7501 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 
     7561 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 
     7621 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 
     7681 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 
     7741 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 
     7801 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 
     7861 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc 
     7921 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 
     7981 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 
     8041 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 
     8101 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 
     8161 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 
     8221 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 
     8281 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 
     8341 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 
     8401 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 
     8461 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 
     8521 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 
     8581 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 
     8641 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 
     8701 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 
     8761 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 
     8821 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 
     8881 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 
     8941 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 
     9001 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 
     9061 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     9121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     9181 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 
     9241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     9301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     9361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     9421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     9481 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     9541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     9601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     9661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     9721 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 
     9781 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 
     9841 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 
     9901 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt 
     9961 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt 
    10021 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag 
    10081 cgcgcaatta accctcacta aagggaacaa aagctggagc tgc 
//
