LOCUS       pLV-puro-N-Flag-        9936 bp    DNA     circular     26-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007664|
COMMENT     VNTDBDATE|-13007664|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLV-puro-N-Flag-Snrnp40|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     CDS             7857..8717
                     /gene=Amp(R)
                     /vntifkey="4"
                     /label=Amp(R)
     promoter        8918..9458
                     /gene=pUC ori
                     /vntifkey="30"
                     /label=pUC\ori
     promoter        7..235
                     /gene=RSV
                     /vntifkey="30"
                     /label=RSV
     LTR             6462..6696
                     /gene=3' LTR
                     /vntifkey="19"
                     /label=3'\LTR
     intron          526..1690
                     /gene=Intron
                     /vntifkey="15"
                     /label=Intron
     rep_origin      complement(7271..7726)
                     /gene=f1 ori
                     /vntifkey="33"
                     /label=f1\ori
     LTR             236..416
                     /gene=5'LTR
                     /vntifkey="19"
                     /label=5'LTR
     polyA_signal    6768..6898
                     /gene=SV40 pA
                     /vntifkey="25"
                     /label=SV40\pA
     misc_feature    6899..7112
                     /gene=SV40 ori
                     /vntifkey="21"
                     /label=SV40\ori
     misc_feature    5783..6379
                     /gene=WPRE
                     /vntifkey="21"
                     /label=WPRE
     promoter        1933..2327
                     /gene=human EF1a promoter
                     /vntifkey="30"
                     /label=human\EF1a\promoter
     intron          2361..3303
                     /gene=intron
                     /vntifkey="15"
                     /label=intron
     CDS             5174..5773
                     /gene=Puro
                     /vntifkey="4"
                     /label=Puro
     RBS             4566..5150
                     /gene=EMCV IRES
                     /vntifkey="32"
                     /label=IRES
     misc_feature    3348..3374
                     /vntifkey="21"
                     /label=N-flag
     misc_feature    3381..4457
                     /vntifkey="21"
                     /label=Snrnp40
BASE COUNT     2378 a      2536 c      2678 g      2344 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctgagctcc ttaaggttaa cgccaccatg gactacaaag 
     3361 acgatgacga caagtctaga atgatcgagc agcagaagcg taagggcccg gagctacccc 
     3421 tggttcccgt caagcggccg cgacatgagt tgctgttggg agcggcagga gccggccccg 
     3481 gagcggggcc gcagcaggcg actccagggg cattgctgca ggcgggaccg cccaggtgtt 
     3541 cttcccttca agctccaatc atgctgctct cgggacatga gggggaagtg tactgctgca 
     3601 agtttcaccc caatggatcc accctggctt ctgcaggatt tgaccggcta atactgttgt 
     3661 ggaacgtcta tggagactgt gataactatg cgacgttgaa gggacacagt ggagcagtga 
     3721 tggagctgca ctataacaca gatggcagca tgctcttctc agcgtcgaca gacaaaactg 
     3781 tggcggtgtg ggatagtgaa actggggaga gagtgaaaag gctaaagggg catacttcct 
     3841 ttgtgaactc ctgttaccca gccaggcggg gcccccaact tgtctgtact ggcagtgacg 
     3901 atggtacagt taagctttgg gacatccgga agaaagcagc tgtccagaca tttcagaaca 
     3961 cgtaccaggt gttggctgtg accttcaatg acacgagcga tcagattatc tctggtggaa 
     4021 tagataatga catcaaggtc tgggacctac gccaaaacaa gttaacctac accatgagag 
     4081 gccatgcaga ctctgtaact ggcctgagct tgagttctga aggctcttat ctcctgtcca 
     4141 atgcaatgga caacacagtt cgtgtctggg atgtccggcc atttgctccc aaagagagat 
     4201 gtgtgaagat atttcaagga aatgtccaca actttgaaaa gaacctcctg agatgttcct 
     4261 ggtctccaga tggaagcaag atagcagctg gctcagcaga caggtttgtg tacgtgtggg 
     4321 acaccacaag ccgaagggtc ctgtacaagc tgcctggtca cgctggctcc atcaatgaag 
     4381 tggctttcca ccccgacgag cccatcatcc tctcagcatc cagtgacaag aggctgtaca 
     4441 tgggtgagat ccagtgagga tccaatattc ccgggctcga gccatggaag cttgatatct 
     4501 aactgactga accggtggta ccgatccacg cgtctccggc ctagggataa cagggtaatc 
     4561 cgctagcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg 
     4621 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag 
     4681 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc 
     4741 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg 
     4801 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag 
     4861 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca 
     4921 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt 
     4981 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc 
     5041 tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa 
     5101 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca ctagagatcc 
     5161 accggtcgcc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 
     5221 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 
     5281 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 
     5341 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 
     5401 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 
     5461 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 
     5521 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 
     5581 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 
     5641 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 
     5701 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 
     5761 gcccggtgcc tgagcggccg cgtcgacaat caacctctgg attacaaaat ttgtgaaaga 
     5821 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 
     5881 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 
     5941 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 
     6001 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 
     6061 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 
     6121 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 
     6181 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 
     6241 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 
     6301 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 
     6361 tgggccgcct ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg 
     6421 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 
     6481 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 
     6541 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 
     6601 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 
     6661 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 
     6721 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 
     6781 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 
     6841 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 
     6901 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     6961 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 
     7021 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat 
     7081 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac 
     7141 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 
     7201 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 
     7261 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 
     7321 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 
     7381 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 
     7441 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 
     7501 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 
     7561 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 
     7621 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 
     7681 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc 
     7741 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 
     7801 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 
     7861 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 
     7921 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 
     7981 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 
     8041 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 
     8101 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 
     8161 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 
     8221 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 
     8281 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 
     8341 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 
     8401 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 
     8461 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 
     8521 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 
     8581 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 
     8641 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 
     8701 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 
     8761 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 
     8821 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     8881 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     8941 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     9001 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 
     9061 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     9121 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     9181 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     9241 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     9301 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     9361 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     9421 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     9481 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 
     9541 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 
     9601 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 
     9661 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 
     9721 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 
     9781 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 
     9841 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa 
     9901 ttaaccctca ctaaagggaa caaaagctgg agctgc 
//
