LOCUS       pBOB-C-Flag-GK5_       10459 bp    DNA     circular      6-FEB-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12148616|
COMMENT     VNTDBDATE|-12148616|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBOB-C-Flag-GK5_v2|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     primer          3033..3058
                     /vntifkey="27"
                     /label=pLNCX5'\primer
     misc_feature    4764..4790
                     /vntifkey="21"
                     /label=C-Flag
     misc_feature    3144..4745
                     /vntifkey="21"
                     /label=GK5_v2
BASE COUNT     2643 a      2529 c      2700 g      2587 t 
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat 
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg 
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct 
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga 
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg 
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa 
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg 
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg 
     3121 ttaactctag agaattcgga tccatgtcag ggcagcagga gcgtgcagag cggcagcgag 
     3181 aggaactgag cgcgtcggcc tcgccgccct cccgcttcgt gctggggctg gacgtgggca 
     3241 gcacggtgat ccgctgccac gtctatgacc agacggcgcg agtgcgaggc tccagcgcgc 
     3301 agaaggtaga aaatgtttac cctcaacctg gctgggttga aatcgatcct gattctctct 
     3361 gggctcagtt tgttgccgta ataaaagatg ctgtcaaagc tgcaggggtg cagatgaatc 
     3421 aaattgtcgg tcttggcata tcaacacaga gagccacttt cattacatgg aacaagaaaa 
     3481 caggccatca ttttcacaac ttcattagct ggcaagacct aagggcagct gaacttgtaa 
     3541 agtcctggaa taattctctc ataatgaagc tattgcatgg ggccacccga gtccttcatt 
     3601 tcttcagtag aagtaaagta atgctaacgg tcagccgctt caatttcagc acccagcatg 
     3661 ccaccttaag attgacctgg attttacaaa acctatctga ggtgaaaagg gcagttgaag 
     3721 aagacaattg ttgctttggg acgattgata cctggctgtt atacaagctc acgaaaggtt 
     3781 cttcctacgc cacggattat tcaaatgcca gcaccactgg attttttgat ccctatgcga 
     3841 tgcgctggag caggctcata accaccatgg tctccatccc actgtctatc ctgcctcctg 
     3901 tgaaggatac cagctataat tttggatcag tggatgagaa gatatttggc gtgcctatac 
     3961 cagttgttgc cttggttggt gaccagcagt cggccatgtt tggagagtgc tgcttcgaga 
     4021 cgggagatgt gaagctaacc atgggaactg gaacattttt ggatatcaat actggaaaaa 
     4081 accttcagca tgttaatgga ggcttttatc cactaattgg atggaagatt gggcaagaac 
     4141 ttgtatgttt agctgaaggc aatgcaggag acacgggcac cgccataatg tgggctcaaa 
     4201 aattagatct cttcacagac gctgctgaga ctgaaaaaat ggccctgagc ctggaagatt 
     4261 ctgaaggggt ttactttgtt ccgtcgttta gtggattaca ggctccatta aatgacccct 
     4321 gtgcctgtgc ctcttttatg ggtttgaagc actccactaa taaataccat cttgtccgag 
     4381 caatattgga gtcaatagct ttcaggaaca aacagttgta cgacatgctg cagagagaaa 
     4441 tccagattcc cgtcacgaac atccgggcag atggaggcgt gtgtaacaac gctttcgtca 
     4501 tgcagatgac ttcagacctg ataaatgaga agatagacag acctgcccac ttcgacatgt 
     4561 cctgcttggg ggcagcctct ctggctggcc tcgctgttgg attttgggcg gacaaggaag 
     4621 agctccagaa gctgaggcaa agcgagatgg ttttcaagcc tcagaagaag tggcaggaat 
     4681 acgaggtgaa tatggaaaac tgggtaaaag cggtgaagcg ctccatgaat tggtataaca 
     4741 agacgctcga gccatggaag cttatggact acaaagacga tgacgacaag gatatctaac 
     4801 tgactgaacc ggtggtaccg aggaattaat tcgctgtctg cgagggccag ctgttggggt 
     4861 gagtactccc tctcaaaagc gggcatgact tctgcgctaa gattgtcagt ttccaaaaac 
     4921 gaggaggatt tgatattcac ctggcccgcg gtgatgcctt tgagggtggc cgcgtccatc 
     4981 tggtcagaaa agacaatctt tttgttgtca agcttgaggt gtggcaggct tgagatctgg 
     5041 ccatacactt gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc 
     5101 aggtccaact gcaggtcgag catgcatcta gggcggccaa ttccgccgat cctggaaaaa 
     5161 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa 
     5221 gcacaagagg aggaggaggt gggttttcca gtcacacctc agacaatcaa cctctggatt 
     5281 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 
     5341 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 
     5401 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 
     5461 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 
     5521 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 
     5581 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 
     5641 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 
     5701 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 
     5761 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 
     5821 cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcgg tacctttaag 
     5881 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 
     5941 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac 
     6001 acaaggctac ttccctgatt gacagaacta cacaccaggg ccaggggtca gatatccact 
     6061 gacctttgga tggtgctaca agctagtacc agttgagcca gataagatag aagaggccaa 
     6121 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga 
     6181 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga 
     6241 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 
     6301 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 
     6361 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 
     6421 agtgtggaaa atctctagca gggccgcttt aaacccgctg atcagcctcg actgtgcctt 
     6481 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 
     6541 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 
     6601 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 
     6661 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct 
     6721 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 
     6781 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 
     6841 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca 
     6901 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 
     6961 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 
     7021 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 
     7081 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg 
     7141 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg 
     7201 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt 
     7261 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 
     7321 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 
     7381 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 
     7441 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 
     7501 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg 
     7561 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa 
     7621 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg 
     7681 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt 
     7741 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt 
     7801 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg 
     7861 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg 
     7921 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt 
     7981 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta 
     8041 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 
     8101 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 
     8161 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 
     8221 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 
     8281 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     8341 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     8401 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     8461 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     8521 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     8581 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     8641 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     8701 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     8761 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     8821 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     8881 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     8941 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     9001 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     9061 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     9121 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 
     9181 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     9241 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     9301 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     9361 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     9421 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     9481 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     9541 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     9601 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     9661 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     9721 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     9781 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     9841 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     9901 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     9961 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
    10021 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
    10081 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
    10141 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
    10201 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
    10261 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
    10321 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
    10381 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
    10441 aaaagtgcca cctgacgtc 
//
