LOCUS       pBOB-C-Flag-GK5\        9718 bp    DNA     circular     26-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007678|
COMMENT     VNTDBDATE|-13007689|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBOB-C-Flag-GK5 (1-287)|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     primer          3033..3058
                     /vntifkey="27"
                     /label=pLNCX5'\primer
     misc_feature    4023..4049
                     /vntifkey="21"
                     /label=C-Flag
     misc_feature    3147..4004
                     /vntifkey="21"
                     /label=GK5\(1-287)
BASE COUNT     2431 a      2379 c      2500 g      2408 t 
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat 
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg 
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct 
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga 
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg 
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa 
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg 
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg 
     3121 ttaactctag agaattcgga tccatgtcag ggcagcagga gcgtgcagag cggcagcgag 
     3181 aggaactgag cgcgtcggcc tcgccgccct cccgcttcgt gctggggctg gacgtgggca 
     3241 gcacggtgat ccgctgccac gtctatgacc agacggcgcg agtgcgaggc tccagcgcgc 
     3301 agaaggtaga aaatgtttac cctcaacctg gctgggttga aatcgatcct gattctctct 
     3361 gggctcagtt tgttgccgta ataaaagatg ctgtcaaagc tgcaggggtg cagatgaatc 
     3421 aaattgtcgg tcttggcata tcaacacaga gagccacttt cattacatgg aacaagaaaa 
     3481 caggccatca ttttcacaac ttcattagct ggcaagacct aagggcagct gaacttgtaa 
     3541 agtcctggaa taattctctc ataatgaagc tattgcatgg ggccacccga gtccttcatt 
     3601 tcttcagtag aagtaaagta atgctaacgg tcagccgctt caatttcagc acccagcatg 
     3661 ccaccttaag attgacctgg attttacaaa acctatctga ggtgaaaagg gcagttgaag 
     3721 aagacaattg ttgctttggg acgattgata cctggctgtt atacaagctc acgaaaggtt 
     3781 cttcctacgc cacggattat tcaaatgcca gcaccactgg attttttgat ccctatgcga 
     3841 tgcgctggag caggctcata accaccatgg tctccatccc actgtctatc ctgcctcctg 
     3901 tgaaggatac cagctataat tttggatcag tggatgagaa gatatttggc gtgcctatac 
     3961 cagttgttgc cttggttggt gaccagcagt cggccatgtt tggactcgag ccatggaagc 
     4021 ttatggacta caaagacgat gacgacaagg atatctaact gactgaaccg gtggtaccga 
     4081 ggaattaatt cgctgtctgc gagggccagc tgttggggtg agtactccct ctcaaaagcg 
     4141 ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg aggaggattt gatattcacc 
     4201 tggcccgcgg tgatgccttt gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt 
     4261 ttgttgtcaa gcttgaggtg tggcaggctt gagatctggc catacacttg agtgacaatg 
     4321 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg caggtcgagc 
     4381 atgcatctag ggcggccaat tccgccgatc ctggaaaaac atggagcaat cacaagtagc 
     4441 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg 
     4501 ggttttccag tcacacctca gacaatcaac ctctggatta caaaatttgt gaaagattga 
     4561 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 
     4621 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 
     4681 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 
     4741 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 
     4801 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 
     4861 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 
     4921 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 
     4981 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 
     5041 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 
     5101 ccgcctcccc gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc 
     5161 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 
     5221 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg 
     5281 acagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa 
     5341 gctagtacca gttgagccag ataagataga agaggccaat aaaggagaga acaccagctt 
     5401 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag 
     5461 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg actgtactgg 
     5521 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 
     5581 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 
     5641 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 
     5701 ggccgcttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 
     5761 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 
     5821 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 
     5881 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 
     5941 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 
     6001 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 
     6061 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 
     6121 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta 
     6181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 
     6241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 
     6301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 
     6361 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 
     6421 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 
     6481 aggcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 
     6541 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 
     6601 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 
     6661 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 
     6721 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 
     6781 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc 
     6841 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac 
     6901 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga 
     6961 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga 
     7021 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg 
     7081 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa 
     7141 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga 
     7201 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg 
     7261 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 
     7321 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 
     7381 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
     7441 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 
     7501 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg 
     7561 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 
     7621 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 
     7681 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 
     7741 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 
     7801 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
     7861 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
     7921 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
     7981 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
     8041 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
     8101 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg 
     8161 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
     8221 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
     8281 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
     8341 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
     8401 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
     8461 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
     8521 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
     8581 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
     8641 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
     8701 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
     8761 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
     8821 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
     8881 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
     8941 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
     9001 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 
     9061 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
     9121 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
     9181 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
     9241 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
     9301 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
     9361 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
     9421 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
     9481 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
     9541 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
     9601 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
     9661 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc 
//
