LOCUS       pBOB-C-Flag-GK5\        9649 bp    DNA     circular     26-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007681|
COMMENT     VNTDBDATE|-13007690|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBOB-C-Flag-GK5 (25-287)|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     primer          3033..3058
                     /vntifkey="27"
                     /label=pLNCX5'\primer
     misc_feature    3954..3980
                     /vntifkey="21"
                     /label=C-Flag
     misc_feature    3147..3935
                     /vntifkey="21"
                     /label=GK5\(25-287)
BASE COUNT     2419 a      2355 c      2473 g      2402 t 
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat 
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg 
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct 
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga 
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg 
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa 
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg 
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg 
     3121 ttaactctag agaattcgga tccatgttcg tgctggggct ggacgtgggc agcacggtga 
     3181 tccgctgcca cgtctatgac cagacggcgc gagtgcgagg ctccagcgcg cagaaggtag 
     3241 aaaatgttta ccctcaacct ggctgggttg aaatcgatcc tgattctctc tgggctcagt 
     3301 ttgttgccgt aataaaagat gctgtcaaag ctgcaggggt gcagatgaat caaattgtcg 
     3361 gtcttggcat atcaacacag agagccactt tcattacatg gaacaagaaa acaggccatc 
     3421 attttcacaa cttcattagc tggcaagacc taagggcagc tgaacttgta aagtcctgga 
     3481 ataattctct cataatgaag ctattgcatg gggccacccg agtccttcat ttcttcagta 
     3541 gaagtaaagt aatgctaacg gtcagccgct tcaatttcag cacccagcat gccaccttaa 
     3601 gattgacctg gattttacaa aacctatctg aggtgaaaag ggcagttgaa gaagacaatt 
     3661 gttgctttgg gacgattgat acctggctgt tatacaagct cacgaaaggt tcttcctacg 
     3721 ccacggatta ttcaaatgcc agcaccactg gattttttga tccctatgcg atgcgctgga 
     3781 gcaggctcat aaccaccatg gtctccatcc cactgtctat cctgcctcct gtgaaggata 
     3841 ccagctataa ttttggatca gtggatgaga agatatttgg cgtgcctata ccagttgttg 
     3901 ccttggttgg tgaccagcag tcggccatgt ttggactcga gccatggaag cttatggact 
     3961 acaaagacga tgacgacaag gatatctaac tgactgaacc ggtggtaccg aggaattaat 
     4021 tcgctgtctg cgagggccag ctgttggggt gagtactccc tctcaaaagc gggcatgact 
     4081 tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac ctggcccgcg 
     4141 gtgatgcctt tgagggtggc cgcgtccatc tggtcagaaa agacaatctt tttgttgtca 
     4201 agcttgaggt gtggcaggct tgagatctgg ccatacactt gagtgacaat gacatccact 
     4261 ttgcctttct ctccacaggt gtccactccc aggtccaact gcaggtcgag catgcatcta 
     4321 gggcggccaa ttccgccgat cctggaaaaa catggagcaa tcacaagtag caatacagca 
     4381 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca 
     4441 gtcacacctc agacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 
     4501 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 
     4561 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 
     4621 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 
     4681 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 
     4741 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 
     4801 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 
     4861 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 
     4921 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 
     4981 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 
     5041 cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct 
     5101 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aaagaagaca 
     5161 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt gacagaacta 
     5221 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc 
     5281 agttgagcca gataagatag aagaggccaa taaaggagag aacaccagct tgttacaccc 
     5341 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag 
     5401 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gactgtactg ggtctctctg 
     5461 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 
     5521 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 
     5581 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggccgcttt 
     5641 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 
     5701 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 
     5761 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 
     5821 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 
     5881 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 
     5941 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 
     6001 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 
     6061 gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac 
     6121 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 
     6181 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 
     6241 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 
     6301 tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 
     6361 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca 
     6421 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 
     6481 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 
     6541 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 
     6601 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 
     6661 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 
     6721 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat 
     6781 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt 
     6841 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg 
     6901 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct 
     6961 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt 
     7021 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga 
     7081 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct 
     7141 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct 
     7201 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 
     7261 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 
     7321 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 
     7381 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 
     7441 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 
     7501 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 
     7561 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 
     7621 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 
     7681 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 
     7741 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     7801 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
     7861 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
     7921 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
     7981 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
     8041 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg 
     8101 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
     8161 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
     8221 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
     8281 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 
     8341 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
     8401 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
     8461 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
     8521 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
     8581 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
     8641 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
     8701 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
     8761 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
     8821 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
     8881 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
     8941 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 
     9001 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
     9061 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
     9121 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
     9181 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
     9241 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 
     9301 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
     9361 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
     9421 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
     9481 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
     9541 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     9601 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc 
//
