LOCUS       pBOB-C-Flag-GK5\        9604 bp    DNA     circular     26-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007685|
COMMENT     VNTDBDATE|-13007691|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBOB-C-Flag-GK5 (287-534)|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     primer          3033..3058
                     /vntifkey="27"
                     /label=pLNCX5'\primer
     misc_feature    3909..3935
                     /vntifkey="21"
                     /label=C-Flag
     misc_feature    3147..3890
                     /vntifkey="21"
                     /label=GK5\(287-534)
BASE COUNT     2429 a      2320 c      2481 g      2374 t 
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat 
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg 
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct 
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga 
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg 
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa 
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg 
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg 
     3121 ttaactctag agaattcgga tccatgggag agtgctgctt cgagacggga gatgtgaagc 
     3181 taaccatggg aactggaaca tttttggata tcaatactgg aaaaaacctt cagcatgtta 
     3241 atggaggctt ttatccacta attggatgga agattgggca agaacttgta tgtttagctg 
     3301 aaggcaatgc aggagacacg ggcaccgcca taatgtgggc tcaaaaatta gatctcttca 
     3361 cagacgctgc tgagactgaa aaaatggccc tgagcctgga agattctgaa ggggtttact 
     3421 ttgttccgtc gtttagtgga ttacaggctc cattaaatga cccctgtgcc tgtgcctctt 
     3481 ttatgggttt gaagcactcc actaataaat accatcttgt ccgagcaata ttggagtcaa 
     3541 tagctttcag gaacaaacag ttgtacgaca tgctgcagag agaaatccag attcccgtca 
     3601 cgaacatccg ggcagatgga ggcgtgtgta acaacgcttt cgtcatgcag atgacttcag 
     3661 acctgataaa tgagaagata gacagacctg cccacttcga catgtcctgc ttgggggcag 
     3721 cctctctggc tggcctcgct gttggatttt gggcggacaa ggaagagctc cagaagctga 
     3781 ggcaaagcga gatggttttc aagcctcaga agaagtggca ggaatacgag gtgaatatgg 
     3841 aaaactgggt aaaagcggtg aagcgctcca tgaattggta taacaagacg ctcgagccat 
     3901 ggaagcttat ggactacaaa gacgatgacg acaaggatat ctaactgact gaaccggtgg 
     3961 taccgaggaa ttaattcgct gtctgcgagg gccagctgtt ggggtgagta ctccctctca 
     4021 aaagcgggca tgacttctgc gctaagattg tcagtttcca aaaacgagga ggatttgata 
     4081 ttcacctggc ccgcggtgat gcctttgagg gtggccgcgt ccatctggtc agaaaagaca 
     4141 atctttttgt tgtcaagctt gaggtgtggc aggcttgaga tctggccata cacttgagtg 
     4201 acaatgacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcagg 
     4261 tcgagcatgc atctagggcg gccaattccg ccgatcctgg aaaaacatgg agcaatcaca 
     4321 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag 
     4381 gaggtgggtt ttccagtcac acctcagaca atcaacctct ggattacaaa atttgtgaaa 
     4441 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 
     4501 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 
     4561 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 
     4621 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 
     4681 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 
     4741 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 
     4801 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 
     4861 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 
     4921 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 
     4981 tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa 
     5041 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 
     5101 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc 
     5161 tgattgacag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg 
     5221 ctacaagcta gtaccagttg agccagataa gatagaagag gccaataaag gagagaacac 
     5281 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga 
     5341 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggactg 
     5401 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 
     5461 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 
     5521 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 
     5581 tagcagggcc gctttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat 
     5641 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc 
     5701 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg 
     5761 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg 
     5821 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt 
     5881 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 
     5941 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 
     6001 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc 
     6061 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 
     6121 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 
     6181 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 
     6241 atttataagg gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa 
     6301 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 
     6361 ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 
     6421 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 
     6481 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 
     6541 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg 
     6601 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 
     6661 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc 
     6721 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa 
     6781 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg 
     6841 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg 
     6901 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct 
     6961 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc 
     7021 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg 
     7081 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca 
     7141 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 
     7201 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 
     7261 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     7321 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
     7381 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta 
     7441 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 
     7501 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 
     7561 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 
     7621 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 
     7681 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 
     7741 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 
     7801 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 
     7861 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 
     7921 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 
     7981 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 
     8041 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 
     8101 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 
     8161 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 
     8221 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 
     8281 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 
     8341 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 
     8401 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 
     8461 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 
     8521 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 
     8581 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 
     8641 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 
     8701 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 
     8761 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 
     8821 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 
     8881 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 
     8941 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 
     9001 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 
     9061 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 
     9121 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 
     9181 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 
     9241 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 
     9301 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 
     9361 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 
     9421 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 
     9481 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 
     9541 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 
     9601 cgtc 
//
