LOCUS       pBOB-C-Flag-GK5\        9190 bp    DNA     circular     26-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007687|
COMMENT     VNTDBDATE|-13007688|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBOB-C-Flag-GK5 (287-396)|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     primer          3033..3058
                     /vntifkey="27"
                     /label=pLNCX5'\primer
     misc_feature    3495..3521
                     /vntifkey="21"
                     /label=C-Flag
     misc_feature    3147..3476
                     /vntifkey="21"
                     /label=GK5\(287-396)
BASE COUNT     2308 a      2233 c      2368 g      2281 t 
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat 
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg 
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct 
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga 
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg 
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa 
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg 
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg 
     3121 ttaactctag agaattcgga tccatgggag agtgctgctt cgagacggga gatgtgaagc 
     3181 taaccatggg aactggaaca tttttggata tcaatactgg aaaaaacctt cagcatgtta 
     3241 atggaggctt ttatccacta attggatgga agattgggca agaacttgta tgtttagctg 
     3301 aaggcaatgc aggagacacg ggcaccgcca taatgtgggc tcaaaaatta gatctcttca 
     3361 cagacgctgc tgagactgaa aaaatggccc tgagcctgga agattctgaa ggggtttact 
     3421 ttgttccgtc gtttagtgga ttacaggctc cattaaatga cccctgtgcc tgtgccctcg 
     3481 agccatggaa gcttatggac tacaaagacg atgacgacaa ggatatctaa ctgactgaac 
     3541 cggtggtacc gaggaattaa ttcgctgtct gcgagggcca gctgttgggg tgagtactcc 
     3601 ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat 
     3661 ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat ctggtcagaa 
     3721 aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg gccatacact 
     3781 tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac 
     3841 tgcaggtcga gcatgcatct agggcggcca attccgccga tcctggaaaa acatggagca 
     3901 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag 
     3961 gaggaggagg tgggttttcc agtcacacct cagacaatca acctctggat tacaaaattt 
     4021 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 
     4081 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 
     4141 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 
     4201 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 
     4261 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 
     4321 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 
     4381 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc 
     4441 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 
     4501 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 
     4561 tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac 
     4621 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 
     4681 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta 
     4741 cttccctgat tgacagaact acacaccagg gccaggggtc agatatccac tgacctttgg 
     4801 atggtgctac aagctagtac cagttgagcc agataagata gaagaggcca ataaaggaga 
     4861 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt 
     4921 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc 
     4981 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 
     5041 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 
     5101 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 
     5161 aatctctagc agggccgctt taaacccgct gatcagcctc gactgtgcct tctagttgcc 
     5221 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 
     5281 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 
     5341 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 
     5401 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta 
     5461 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 
     5521 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 
     5581 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag 
     5641 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 
     5701 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 
     5761 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 
     5821 cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt 
     5881 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc 
     5941 cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 
     6001 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 
     6061 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 
     6121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 
     6181 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 
     6241 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa 
     6301 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat 
     6361 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga 
     6421 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt 
     6481 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa 
     6541 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt 
     6601 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc 
     6661 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga 
     6721 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt 
     6781 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 
     6841 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 
     6901 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
     6961 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 
     7021 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 
     7081 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 
     7141 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 
     7201 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 
     7261 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
     7321 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 
     7381 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     7441 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     7501 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     7561 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     7621 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     7681 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     7741 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     7801 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     7861 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     7921 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     7981 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     8041 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     8101 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     8161 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     8221 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     8281 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     8341 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     8401 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     8461 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     8521 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     8581 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     8641 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     8701 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     8761 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     8821 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     8881 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     8941 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     9001 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     9061 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     9121 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     9181 acctgacgtc 
//
