LOCUS       pBOB-C-Flag-GK5\        9277 bp    DNA     circular     26-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007695|
COMMENT     VNTDBDATE|-13007695|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBOB-C-Flag-GK5 (396-534)|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     primer          3033..3058
                     /vntifkey="27"
                     /label=pLNCX5'\primer
     misc_feature    3582..3608
                     /vntifkey="21"
                     /label=C-Flag
     misc_feature    3147..3563
                     /vntifkey="21"
                     /label=GK5\(396-534)
BASE COUNT     2337 a      2259 c      2393 g      2288 t 
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat 
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg 
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct 
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga 
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg 
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa 
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg 
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg 
     3121 ttaactctag agaattcgga tccatggcct cttttatggg tttgaagcac tccactaata 
     3181 aataccatct tgtccgagca atattggagt caatagcttt caggaacaaa cagttgtacg 
     3241 acatgctgca gagagaaatc cagattcccg tcacgaacat ccgggcagat ggaggcgtgt 
     3301 gtaacaacgc tttcgtcatg cagatgactt cagacctgat aaatgagaag atagacagac 
     3361 ctgcccactt cgacatgtcc tgcttggggg cagcctctct ggctggcctc gctgttggat 
     3421 tttgggcgga caaggaagag ctccagaagc tgaggcaaag cgagatggtt ttcaagcctc 
     3481 agaagaagtg gcaggaatac gaggtgaata tggaaaactg ggtaaaagcg gtgaagcgct 
     3541 ccatgaattg gtataacaag acgctcgagc catggaagct tatggactac aaagacgatg 
     3601 acgacaagga tatctaactg actgaaccgg tggtaccgag gaattaattc gctgtctgcg 
     3661 agggccagct gttggggtga gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga 
     3721 ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg 
     3781 agggtggccg cgtccatctg gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt 
     3841 ggcaggcttg agatctggcc atacacttga gtgacaatga catccacttt gcctttctct 
     3901 ccacaggtgt ccactcccag gtccaactgc aggtcgagca tgcatctagg gcggccaatt 
     3961 ccgccgatcc tggaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct 
     4021 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag 
     4081 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 
     4141 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 
     4201 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 
     4261 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 
     4321 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 
     4381 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 
     4441 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 
     4501 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 
     4561 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 
     4621 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc 
     4681 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 
     4741 aaaagaaaag gggggactgg aagggctaat tcactcccaa agaagacaag atatccttga 
     4801 tctgtggatc taccacacac aaggctactt ccctgattga cagaactaca caccagggcc 
     4861 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga 
     4921 taagatagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca 
     4981 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt 
     5041 tcatcacgtg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat 
     5101 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 
     5161 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 
     5221 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gccgctttaa acccgctgat 
     5281 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 
     5341 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 
     5401 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 
     5461 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 
     5521 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat 
     5581 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 
     5641 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 
     5701 aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc 
     5761 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 
     5821 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 
     5881 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg 
     5941 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa 
     6001 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa 
     6061 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 
     6121 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 
     6181 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     6241 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 
     6301 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 
     6361 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata 
     6421 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac 
     6481 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga 
     6541 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc 
     6601 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga 
     6661 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc 
     6721 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc 
     6781 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga 
     6841 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 
     6901 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat 
     6961 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 
     7021 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 
     7081 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 
     7141 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 
     7201 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 
     7261 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 
     7321 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 
     7381 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 
     7441 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 
     7501 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 
     7561 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 
     7621 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 
     7681 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag 
     7741 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 
     7801 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 
     7861 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 
     7921 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 
     7981 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 
     8041 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 
     8101 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 
     8161 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 
     8221 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 
     8281 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 
     8341 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 
     8401 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 
     8461 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 
     8521 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 
     8581 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 
     8641 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 
     8701 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 
     8761 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 
     8821 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 
     8881 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 
     8941 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 
     9001 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 
     9061 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 
     9121 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 
     9181 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 
     9241 tccgcgcaca tttccccgaa aagtgccacc tgacgtc 
//
