LOCUS       pBOB-C-3HA-GK5_v       10471 bp    DNA     circular     13-NOV-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|750441120|
COMMENT     VNTDBDATE|750441120|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBOB-C-3HA-GK5_v1|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     primer          3033..3058
                     /vntifkey="27"
                     /label=pLNCX5'\primer
     misc_feature    4710..4802
                     /vntifkey="21"
                     /label=C-3HA
     misc_feature    3144..4691
                     /vntifkey="21"
                     /label=GK5_v1
BASE COUNT     2630 a      2544 c      2695 g      2602 t 
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat 
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg 
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct 
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga 
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg 
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa 
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg 
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg 
     3121 ttaactctag agaattcgga tccatgtcag ggcagcagga gcgtgcagag cggcagcgag 
     3181 aggaactgag cgcgtcggcc tcgccgccct cccgcttcgt gctggggctg gacgtgggca 
     3241 gcacggtgat ccgctgccac gtctatgacc agacggcgcg agtgcgaggc tccagcgcgc 
     3301 agaaggtaga aaatgtttac cctcaacctg gctgggttga aatcgatcct gattctctct 
     3361 gggctcagtt tgttgccgta ataaaagatg ctgtcaaagc tgcaggggtg cagatgaatc 
     3421 aaattgtcgg tcttggcata tcaacacaga gagccacttt cattacatgg aacaagaaaa 
     3481 caggccatca ttttcacaac ttcattagct ggcaagacct aagggcagct gaacttgtaa 
     3541 agtcctggaa taattctctc ataatgaagc tattgcatgg ggccacccga gtccttcatt 
     3601 tcttcagtag aagtaaagta atgctaacgg tcagccgctt caatttcagc acccagcatg 
     3661 ccaccttaag attgacctgg attttacaaa acctatctga ggtgaaaagg gcagttgaag 
     3721 aagacaattg ttgctttggg acgattgata cctggctgtt atacaagctc acgaaaggtt 
     3781 cttcctacgc cacggattat tcaaatgcca gcaccactgg attttttgat ccctatgcga 
     3841 tgcgctggag caggctcata accaccatgg tctccatccc actgtctatc ctgcctcctg 
     3901 tgaaggatac cagctataat tttggatcag tggatgagaa gatatttggc gtgcctatac 
     3961 cagttgttgc cttggttggt gaccagcagt cggccatgtt tggagagtgc tgcttcgaga 
     4021 cgggagatgt gaagctaacc atgggaactg gaacattttt ggatatcaat actggaaaaa 
     4081 accttcagca tgttaatgga ggcttttatc cactaattgg atggaagatt gggcaagaac 
     4141 ttgtatgttt agctgaaggc aatgcaggag acacgggcac cgccataatg tgggctcaaa 
     4201 aattagatct cttcacagac gctgctgaga ctgaaaaaat ggccctgagc ctggaagatt 
     4261 ctgaaggggt ttactttgtt ccgtcgttta gtggattaca ggctccatta aatgacccct 
     4321 gtgcctgtgc ctcttttatg ggtttgaagc actccactaa taaataccat cttgtccgag 
     4381 caatattgga gtcaatagct ttcaggaaca aacagttgta cgacatgctg cagagagaaa 
     4441 tccagattcc cgtcacgaac atccgggcag atggaggcgt gtgtaacaac gctttcgtca 
     4501 tgcagatgac ttcagacctg ataaatgaga agatagacag acctgcccac ttcgacatgt 
     4561 cctgcttggg ggcagcctct ctggctggcc tcgctgttgg attttgggcg gacaaggaag 
     4621 agctccagaa gctgaggcaa agcgagatgg ttttcaagcc tcagaagaag tggcaggaat 
     4681 acgaggcatt tctcgagcca tggaagcttt acccttatga cgtacctgac tatgctggcg 
     4741 cctaccctta tgacgtacct gactatgctg gataccctta tgacgtacct gactatgctg 
     4801 gagatatcta actgactgaa ccggtggtac cgaggaatta attcgctgtc tgcgagggcc 
     4861 agctgttggg gtgagtactc cctctcaaaa gcgggcatga cttctgcgct aagattgtca 
     4921 gtttccaaaa acgaggagga tttgatattc acctggcccg cggtgatgcc tttgagggtg 
     4981 gccgcgtcca tctggtcaga aaagacaatc tttttgttgt caagcttgag gtgtggcagg 
     5041 cttgagatct ggccatacac ttgagtgaca atgacatcca ctttgccttt ctctccacag 
     5101 gtgtccactc ccaggtccaa ctgcaggtcg agcatgcatc tagggcggcc aattccgccg 
     5161 atcctggaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt 
     5221 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcagacaatc 
     5281 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 
     5341 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 
     5401 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 
     5461 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 
     5521 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 
     5581 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 
     5641 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 
     5701 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 
     5761 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 
     5821 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc 
     5881 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 
     5941 aaagggggga ctggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg 
     6001 gatctaccac acacaaggct acttccctga ttgacagaac tacacaccag ggccaggggt 
     6061 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataagat 
     6121 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat 
     6181 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca 
     6241 cgtggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc 
     6301 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 
     6361 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 
     6421 acccttttag tcagtgtgga aaatctctag cagggccgct ttaaacccgc tgatcagcct 
     6481 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 
     6541 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 
     6601 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 
     6661 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 
     6721 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 
     6781 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 
     6841 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 
     6901 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 
     6961 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 
     7021 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 
     7081 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt 
     7141 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 
     7201 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc 
     7261 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 
     7321 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 
     7381 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 
     7441 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 
     7501 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 
     7561 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga 
     7621 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg 
     7681 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt 
     7741 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca 
     7801 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct 
     7861 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat 
     7921 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa 
     7981 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac 
     8041 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 
     8101 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 
     8161 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 
     8221 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 
     8281 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
     8341 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 
     8401 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 
     8461 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 
     8521 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
     8581 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
     8641 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     8701 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     8761 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     8821 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     8881 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt 
     8941 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     9001 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     9061 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     9121 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 
     9181 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     9241 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     9301 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     9361 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     9421 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     9481 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     9541 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     9601 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     9661 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     9721 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     9781 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     9841 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     9901 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     9961 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
    10021 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
    10081 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
    10141 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
    10201 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
    10261 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
    10321 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
    10381 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
    10441 cacatttccc cgaaaagtgc cacctgacgt c 
//
