LOCUS       pBOB-N-Flag-GK_v       10438 bp    DNA     circular     26-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007709|
COMMENT     VNTDBDATE|-13007709|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBOB-N-Flag-GK_v1|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     primer          3033..3058
                     /vntifkey="27"
                     /label=pLNCX5'\primer
     misc_feature    3132..3158
                     /vntifkey="21"
                     /label=N-flag
     misc_feature    3177..4751
                     /vntifkey="21"
                     /label=GK_v1
BASE COUNT     2664 a      2485 c      2679 g      2610 t 
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat 
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg 
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct 
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga 
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg 
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa 
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg 
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg 
     3121 ttaacgccac catggactac aaagacgatg acgacaagtc tagagaattc ggatccatgg 
     3181 cagccgcgaa gaaagcagtt ctggggccat tggtgggagc agtggaccag ggtaccagct 
     3241 cgacacgttt tttggttttc aattcaaaaa cagctgaact tcttagtcat catcaagtag 
     3301 aaataaaaca ggaattccca agagaaggat gggtagaaca agacccgaag gaaattctgc 
     3361 agtctgttta tgagtgtata gagaaaacgt gtgagaaact tggacagctc aatattgata 
     3421 tttccaacat caaagccatt ggtgtcagca accagaggga aaccacagta gtctgggaca 
     3481 aggtcaccgg agagcctctc tataatgccg tggtgtggct tgacctaaga acccagtcta 
     3541 ctgttgagaa ccttagtaaa agaattccag gaaataataa ctttgtcaag tccaagacag 
     3601 gccttccact tagcacgtat ttcagtgcag tgaaacttcg ttggctcctt gacaacgtga 
     3661 aaaaggtcca agaggctgtt gaagaaaata gagctctttt tgggaccatt gattcatggc 
     3721 ttatttggag tttaacagga ggaatccatg ggggtgtcca ctgtacagat gtaacaaatg 
     3781 caagcaggac gatgcttttt aacattcatt ctttggaatg ggataaagag ctctgcgaat 
     3841 tttttggaat tccaatggaa attcttccca acgttcggag ttcttctgag atctatggcc 
     3901 taatgaaagc tggggccttg gaaggtgtac caatatctgg gtgtttgggg gaccagtctg 
     3961 ctgctttggt gggacaaatg tgcttccagg atggacaggc caaaaacacg tatggaacag 
     4021 ggtgcttctt attgtgcaac acgggccata agtgtgtatt ttctgaacat ggcctcctga 
     4081 caaccgtagc atataaactt ggcagagaca aacctgtgta ttatgcgttg gaaggttccg 
     4141 tggctatagc tggtgctgta atccgctggc taagagacaa ccttggaatt attaagtcct 
     4201 ctgaggaaat tgaaaaactt gctaaggaag taggtacttc ttatggctgc tacttcgttc 
     4261 cagcattttc agggttatat gcgccttatt gggagcccag tgcaagaggg atcatctgtg 
     4321 gactcactca gttcaccaat aaatgtcata tcgcttttgc tgcactagaa gctgtttgtt 
     4381 tccaaacccg agagattttg gatgccatga atcgcgactg tggaattcca ctcagtcatt 
     4441 tacaggtaga tggaggaatg accagcaata aaattcttat gcagctacaa gcagacattc 
     4501 tgtatattcc agtagtgaaa ccctccatgc ctgaaacaac tgcactaggc gctgccatgg 
     4561 cagctggggc tgcagagggg gttggtgtgt ggagtcttga acctgaggat ttgtcagctg 
     4621 tcacaatgga gcggtttgaa cctcagatca atgctgaaga aagcgaaatc cgttactcca 
     4681 catggaagaa agctgtgatg aagtcaattg gttgggttac aactcagtct ccagaaagtg 
     4741 gtatcccata actcgagcca tggaagcttg atatctaact gactgaaccg gtggtaccga 
     4801 ggaattaatt cgctgtctgc gagggccagc tgttggggtg agtactccct ctcaaaagcg 
     4861 ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg aggaggattt gatattcacc 
     4921 tggcccgcgg tgatgccttt gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt 
     4981 ttgttgtcaa gcttgaggtg tggcaggctt gagatctggc catacacttg agtgacaatg 
     5041 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg caggtcgagc 
     5101 atgcatctag ggcggccaat tccgccgatc ctggaaaaac atggagcaat cacaagtagc 
     5161 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg 
     5221 ggttttccag tcacacctca gacaatcaac ctctggatta caaaatttgt gaaagattga 
     5281 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 
     5341 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 
     5401 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 
     5461 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 
     5521 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 
     5581 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 
     5641 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 
     5701 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 
     5761 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 
     5821 ccgcctcccc gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc 
     5881 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 
     5941 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg 
     6001 acagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa 
     6061 gctagtacca gttgagccag ataagataga agaggccaat aaaggagaga acaccagctt 
     6121 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag 
     6181 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg actgtactgg 
     6241 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 
     6301 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 
     6361 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 
     6421 ggccgcttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 
     6481 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 
     6541 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 
     6601 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 
     6661 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 
     6721 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 
     6781 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 
     6841 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta 
     6901 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 
     6961 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 
     7021 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 
     7081 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 
     7141 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 
     7201 aggcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 
     7261 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 
     7321 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 
     7381 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 
     7441 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 
     7501 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc 
     7561 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac 
     7621 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga 
     7681 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga 
     7741 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg 
     7801 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa 
     7861 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga 
     7921 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg 
     7981 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 
     8041 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 
     8101 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
     8161 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 
     8221 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg 
     8281 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 
     8341 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 
     8401 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 
     8461 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 
     8521 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
     8581 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
     8641 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
     8701 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
     8761 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
     8821 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg 
     8881 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
     8941 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
     9001 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
     9061 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
     9121 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
     9181 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
     9241 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
     9301 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
     9361 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
     9421 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
     9481 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
     9541 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
     9601 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
     9661 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
     9721 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 
     9781 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
     9841 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
     9901 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
     9961 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
    10021 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
    10081 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
    10141 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
    10201 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
    10261 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
    10321 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
    10381 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc 
//
