LOCUS       pBOB-N-Flag-GK\(        9445 bp    DNA     circular     26-AUG-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12964302|
COMMENT     VNTDBDATE|-12964306|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBOB-N-Flag-GK (281-473)|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     primer          3033..3058
                     /vntifkey="27"
                     /label=pLNCX5'\primer
     misc_feature    3132..3158
                     /vntifkey="21"
                     /label=N-flag
     misc_feature    3180..3758
                     /vntifkey="21"
                     /label=GK\(281-473)
BASE COUNT     2368 a      2295 c      2426 g      2356 t 
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat 
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg 
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct 
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga 
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg 
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa 
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg 
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg 
     3121 ttaacgccac catggactac aaagacgatg acgacaagtc tagagaattc ggatccatga 
     3181 cagggtgctt cttattgtgc aacacgggcc ataagtgtgt attttctgaa catggcctcc 
     3241 tgacaaccgt agcatataaa cttggcagag acaaacctgt gtattatgcg ttggaaggtt 
     3301 ccgtggctat agctggtgct gtaatccgct ggctaagaga caaccttgga attattaagt 
     3361 cctctgagga aattgaaaaa cttgctaagg aagtaggtac ttcttatggc tgctacttcg 
     3421 ttccagcatt ttcagggtta tatgcgcctt attgggagcc cagtgcaaga gggatcatct 
     3481 gtggactcac tcagttcacc aataaatgtc atatcgcttt tgctgcacta gaagctgttt 
     3541 gtttccaaac ccgagagatt ttggatgcca tgaatcgcga ctgtggaatt ccactcagtc 
     3601 atttacaggt agatggagga atgaccagca ataaaattct tatgcagcta caagcagaca 
     3661 ttctgtatat tccagtagtg aaaccctcca tgcctgaaac aactgcacta ggcgctgcca 
     3721 tggcagctgg ggctgcagag ggggttggtg tgtggagtct cgagccatgg aagcttgata 
     3781 tctaactgac tgaaccggtg gtaccgagga attaattcgc tgtctgcgag ggccagctgt 
     3841 tggggtgagt actccctctc aaaagcgggc atgacttctg cgctaagatt gtcagtttcc 
     3901 aaaaacgagg aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgcg 
     3961 tccatctggt cagaaaagac aatctttttg ttgtcaagct tgaggtgtgg caggcttgag 
     4021 atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc acaggtgtcc 
     4081 actcccaggt ccaactgcag gtcgagcatg catctagggc ggccaattcc gccgatcctg 
     4141 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg 
     4201 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcagac aatcaacctc 
     4261 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 
     4321 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 
     4381 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 
     4441 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 
     4501 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 
     4561 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 
     4621 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 
     4681 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 
     4741 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 
     4801 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc 
     4861 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 
     4921 gggactggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta 
     4981 ccacacacaa ggctacttcc ctgattgaca gaactacaca ccagggccag gggtcagata 
     5041 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata agatagaaga 
     5101 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga 
     5161 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc 
     5221 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga 
     5281 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 
     5341 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 
     5401 ttagtcagtg tggaaaatct ctagcagggc cgctttaaac ccgctgatca gcctcgactg 
     5461 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 
     5521 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 
     5581 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 
     5641 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 
     5701 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg 
     5761 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 
     5821 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 
     5881 ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 
     5941 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 
     6001 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 
     6061 ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa 
     6121 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt 
     6181 agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca 
     6241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 
     6301 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 
     6361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 
     6421 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg 
     6481 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc 
     6541 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt 
     6601 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt 
     6661 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga 
     6721 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca 
     6781 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc 
     6841 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga 
     6901 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt 
     6961 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc 
     7021 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 
     7081 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 
     7141 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
     7201 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 
     7261 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 
     7321 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 
     7381 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     7441 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     7501 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     7561 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
     7621 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     7681 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     7741 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     7801 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     7861 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt 
     7921 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     7981 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     8041 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     8101 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 
     8161 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     8221 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
     8281 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
     8341 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 
     8401 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 
     8461 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 
     8521 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 
     8581 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 
     8641 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 
     8701 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 
     8761 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 
     8821 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 
     8881 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 
     8941 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 
     9001 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 
     9061 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 
     9121 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 
     9181 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 
     9241 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 
     9301 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 
     9361 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
     9421 tccccgaaaa gtgccacctg acgtc 
//
