LOCUS       pBOB-N-3HA-GK_v1       10507 bp    DNA     circular     26-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007714|
COMMENT     VNTDBDATE|-13007714|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBOB-N-3HA-GK_v1|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     primer          3033..3058
                     /vntifkey="27"
                     /label=pLNCX5'\primer
     misc_feature    3126..3227
                     /vntifkey="21"
                     /label=N-3HA
     misc_feature    3246..4820
                     /vntifkey="21"
                     /label=GK_v1
BASE COUNT     2673 a      2507 c      2692 g      2635 t 
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat 
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg 
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct 
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga 
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg 
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa 
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg 
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg 
     3121 ttaacgccac catgtaccct tatgacgtac ctgactatgc tggcgcctac ccttatgacg 
     3181 tacctgacta tgctggatac ccttatgacg tacctgacta tgctggatct agagaattcg 
     3241 gatccatggc agccgcgaag aaagcagttc tggggccatt ggtgggagca gtggaccagg 
     3301 gtaccagctc gacacgtttt ttggttttca attcaaaaac agctgaactt cttagtcatc 
     3361 atcaagtaga aataaaacag gaattcccaa gagaaggatg ggtagaacaa gacccgaagg 
     3421 aaattctgca gtctgtttat gagtgtatag agaaaacgtg tgagaaactt ggacagctca 
     3481 atattgatat ttccaacatc aaagccattg gtgtcagcaa ccagagggaa accacagtag 
     3541 tctgggacaa ggtcaccgga gagcctctct ataatgccgt ggtgtggctt gacctaagaa 
     3601 cccagtctac tgttgagaac cttagtaaaa gaattccagg aaataataac tttgtcaagt 
     3661 ccaagacagg ccttccactt agcacgtatt tcagtgcagt gaaacttcgt tggctccttg 
     3721 acaacgtgaa aaaggtccaa gaggctgttg aagaaaatag agctcttttt gggaccattg 
     3781 attcatggct tatttggagt ttaacaggag gaatccatgg gggtgtccac tgtacagatg 
     3841 taacaaatgc aagcaggacg atgcttttta acattcattc tttggaatgg gataaagagc 
     3901 tctgcgaatt ttttggaatt ccaatggaaa ttcttcccaa cgttcggagt tcttctgaga 
     3961 tctatggcct aatgaaagct ggggccttgg aaggtgtacc aatatctggg tgtttggggg 
     4021 accagtctgc tgctttggtg ggacaaatgt gcttccagga tggacaggcc aaaaacacgt 
     4081 atggaacagg gtgcttctta ttgtgcaaca cgggccataa gtgtgtattt tctgaacatg 
     4141 gcctcctgac aaccgtagca tataaacttg gcagagacaa acctgtgtat tatgcgttgg 
     4201 aaggttccgt ggctatagct ggtgctgtaa tccgctggct aagagacaac cttggaatta 
     4261 ttaagtcctc tgaggaaatt gaaaaacttg ctaaggaagt aggtacttct tatggctgct 
     4321 acttcgttcc agcattttca gggttatatg cgccttattg ggagcccagt gcaagaggga 
     4381 tcatctgtgg actcactcag ttcaccaata aatgtcatat cgcttttgct gcactagaag 
     4441 ctgtttgttt ccaaacccga gagattttgg atgccatgaa tcgcgactgt ggaattccac 
     4501 tcagtcattt acaggtagat ggaggaatga ccagcaataa aattcttatg cagctacaag 
     4561 cagacattct gtatattcca gtagtgaaac cctccatgcc tgaaacaact gcactaggcg 
     4621 ctgccatggc agctggggct gcagaggggg ttggtgtgtg gagtcttgaa cctgaggatt 
     4681 tgtcagctgt cacaatggag cggtttgaac ctcagatcaa tgctgaagaa agcgaaatcc 
     4741 gttactccac atggaagaaa gctgtgatga agtcaattgg ttgggttaca actcagtctc 
     4801 cagaaagtgg tatcccataa ctcgagccat ggaagcttga tatctaactg actgaaccgg 
     4861 tggtaccgag gaattaattc gctgtctgcg agggccagct gttggggtga gtactccctc 
     4921 tcaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg 
     4981 atattcacct ggcccgcggt gatgcctttg agggtggccg cgtccatctg gtcagaaaag 
     5041 acaatctttt tgttgtcaag cttgaggtgt ggcaggcttg agatctggcc atacacttga 
     5101 gtgacaatga catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc 
     5161 aggtcgagca tgcatctagg gcggccaatt ccgccgatcc tggaaaaaca tggagcaatc 
     5221 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag 
     5281 gaggaggtgg gttttccagt cacacctcag acaatcaacc tctggattac aaaatttgtg 
     5341 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 
     5401 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 
     5461 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 
     5521 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 
     5581 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 
     5641 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 
     5701 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg 
     5761 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 
     5821 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 
     5881 ccctttgggc cgcctccccg cctggaattc gagctcggta cctttaagac caatgactta 
     5941 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 
     6001 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt 
     6061 ccctgattga cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg 
     6121 gtgctacaag ctagtaccag ttgagccaga taagatagaa gaggccaata aaggagagaa 
     6181 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt 
     6241 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga 
     6301 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 
     6361 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 
     6421 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 
     6481 ctctagcagg gccgctttaa acccgctgat cagcctcgac tgtgccttct agttgccagc 
     6541 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 
     6601 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 
     6661 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 
     6721 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg 
     6781 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 
     6841 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 
     6901 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt 
     6961 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 
     7021 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 
     7081 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 
     7141 ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac 
     7201 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 
     7261 aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 
     7321 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 
     7381 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 
     7441 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 
     7501 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 
     7561 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta 
     7621 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc 
     7681 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt 
     7741 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt 
     7801 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac 
     7861 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt 
     7921 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg 
     7981 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga 
     8041 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg 
     8101 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 
     8161 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 
     8221 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 
     8281 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 
     8341 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 
     8401 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 
     8461 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 
     8521 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 
     8581 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 
     8641 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 
     8701 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 
     8761 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 
     8821 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 
     8881 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 
     8941 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 
     9001 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 
     9061 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 
     9121 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 
     9181 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 
     9241 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 
     9301 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 
     9361 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 
     9421 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 
     9481 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 
     9541 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 
     9601 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 
     9661 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 
     9721 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 
     9781 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 
     9841 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 
     9901 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 
     9961 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 
    10021 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 
    10081 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 
    10141 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 
    10201 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 
    10261 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 
    10321 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 
    10381 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 
    10441 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 
    10501 tgacgtc 
//
