LOCUS       pBOB-N-3HA-GK_v2       10594 bp    DNA     circular     26-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007715|
COMMENT     VNTDBDATE|-13007715|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBOB-N-3HA-GK_v2|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     primer          3033..3058
                     /vntifkey="27"
                     /label=pLNCX5'\primer
     misc_feature    3126..3227
                     /vntifkey="21"
                     /label=N-3HA
     misc_feature    3246..4907
                     /vntifkey="21"
                     /label=GK_v2
BASE COUNT     2692 a      2522 c      2714 g      2666 t 
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat 
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg 
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct 
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga 
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg 
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa 
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg 
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg 
     3121 ttaacgccac catgtaccct tatgacgtac ctgactatgc tggcgcctac ccttatgacg 
     3181 tacctgacta tgctggatac ccttatgacg tacctgacta tgctggatct agagaattcg 
     3241 gatccatggc agccgcgaag aaagcagttc tggggccatt ggtgggagca gtggaccagg 
     3301 gtaccagctc gacacgtttt ttggttttca attcaaaaac agctgaactt cttagtcatc 
     3361 atcaagtaga aataaaacag gaattcccaa gagaaggatg ggtagaacaa gacccgaagg 
     3421 aaattctgca gtctgtttat gagtgtatag agaaaacgtg tgagaaactt ggacagctca 
     3481 atattgatat ttccaacatc aaagccattg gtgtcagcaa ccagagggaa accacagtag 
     3541 tctgggacaa ggtcaccgga gagcctctct ataatgccgt ggtgtggctt gacctaagaa 
     3601 cccagtctac tgttgagaac cttagtaaaa gaattccagg aaataataac tttgtcaagt 
     3661 ccaagacagg ccttccactt agcacgtatt tcagtgcagt gaaacttcgt tggctccttg 
     3721 acaacgtgaa aaaggtccaa gaggctgttg aagaaaatag agctcttttt gggaccattg 
     3781 attcatggct tatttggagt ttaacaggag gaatccatgg gggtgtccac tgtacagatg 
     3841 taacaaatgc aagcaggacg atgcttttta acattcattc tttggaatgg gataaagagc 
     3901 tctgcgaatt ttttggaatt ccaatggaaa ttcttcccaa cgttcggagt tcttctgaga 
     3961 tctatggcct aatgaaagct ggggccttgg aaggtgtacc aatatctggg tgtttggggg 
     4021 accagtctgc tgctttggtg ggacaaatgt gcttccagga tggacaggcc aaaaacacgt 
     4081 atggaacagg gtgcttctta ttgtgcaaca cgggccataa gtgtgtattt tctgaacatg 
     4141 gcctcctgac aaccgtagca tataaacttg gcagagacaa acctgtgtat tatgcgttgg 
     4201 aaggttccgt ggctatagct ggtgctgtaa tccgctggct aagagacaac cttggaatta 
     4261 ttaagtcctc tgaggaaatt gaaaaacttg ctaaggaagt aggtacttct tatggctgct 
     4321 acttcgttcc agcattttca gggttatatg cgccttattg ggagcccagt gcaagaggga 
     4381 tcatctgtgg actcactcag ttcaccaata aatgtcatat cgcttttgct gcactagaag 
     4441 ctgtttgttt ccaaacccga gagattttgg atgccatgaa tcgcgactgt ggaattccac 
     4501 tcagtcattt acaggtagat ggaggaatga ccagcaataa aattcttatg cagctacaag 
     4561 cagacattct gtatattcca gtagtgaaac cctccatgcc tgaaacaact gcactaggcg 
     4621 ctgccatggc agctggggct gcagaggggg ttggtgtgtg gagtcttgaa cctgaggatt 
     4681 tgtcagctgt cacaatggag cggtttgaac ctcagatcaa tgctgaagaa agcgaaatcc 
     4741 gttactccac atggaagaaa gctgtgatga agtcaattgg ttgggttaca actcagtctc 
     4801 cagaaagtgg tgaccctagt atcttctgta gtctgccttt gggctttttt atagtgagta 
     4861 gcatggtaat gttaatcgga gcaaggtaca tctcaggtat cccataactc gagccatgga 
     4921 agcttgatat ctaactgact gaaccggtgg taccgaggaa ttaattcgct gtctgcgagg 
     4981 gccagctgtt ggggtgagta ctccctctca aaagcgggca tgacttctgc gctaagattg 
     5041 tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgcggtgat gcctttgagg 
     5101 gtggccgcgt ccatctggtc agaaaagaca atctttttgt tgtcaagctt gaggtgtggc 
     5161 aggcttgaga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca 
     5221 caggtgtcca ctcccaggtc caactgcagg tcgagcatgc atctagggcg gccaattccg 
     5281 ccgatcctgg aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat 
     5341 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcagaca 
     5401 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 
     5461 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 
     5521 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 
     5581 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 
     5641 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 
     5701 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 
     5761 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 
     5821 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 
     5881 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 
     5941 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag 
     6001 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 
     6061 agaaaagggg ggactggaag ggctaattca ctcccaaaga agacaagata tccttgatct 
     6121 gtggatctac cacacacaag gctacttccc tgattgacag aactacacac cagggccagg 
     6181 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa 
     6241 gatagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg 
     6301 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca 
     6361 tcacgtggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg 
     6421 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 
     6481 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 
     6541 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc gctttaaacc cgctgatcag 
     6601 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 
     6661 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 
     6721 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 
     6781 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 
     6841 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 
     6901 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     6961 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     7021 ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     7081 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     7141 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     7201 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct 
     7261 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 
     7321 gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca 
     7381 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 
     7441 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 
     7501 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 
     7561 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 
     7621 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 
     7681 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata 
     7741 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc 
     7801 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt 
     7861 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt 
     7921 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga 
     7981 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc 
     8041 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg 
     8101 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc 
     8161 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 
     8221 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 
     8281 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 
     8341 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 
     8401 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 
     8461 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 
     8521 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 
     8581 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 
     8641 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 
     8701 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 
     8761 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 
     8821 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 
     8881 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 
     8941 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 
     9001 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 
     9061 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 
     9121 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 
     9181 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 
     9241 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 
     9301 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 
     9361 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
     9421 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 
     9481 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 
     9541 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 
     9601 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 
     9661 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 
     9721 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 
     9781 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 
     9841 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 
     9901 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 
     9961 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 
    10021 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 
    10081 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 
    10141 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 
    10201 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 
    10261 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 
    10321 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 
    10381 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 
    10441 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 
    10501 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 
    10561 gcgcacattt ccccgaaaag tgccacctga cgtc 
//
