LOCUS       pcDNA6-GK5_v1           6596 bp    DNA     circular SYN 26-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007718|
COMMENT     VNTDBDATE|-13007718|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pcDNA6-GK5_v1|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Invitrogen|
COMMENT     VNTOAUTHORTEL|(800)-357-3114|
COMMENT     VNTOAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTOAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     CDS             complement(5600..6460)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     polyA_site      2558..2782
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     promoter        complement(6461..6559)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             3713..4111
                     /invitrogen="1050000"
                     /vntifkey="4"
                     /label=Bsd(R)
     primer          769..789
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     promoter        232..819
                     /invitrogen="2180000"
                     /vntifkey="29"
                     /label=CMV\promoter
     promoter        3645..3712
                     /invitrogen="2340000"
                     /vntifkey="30"
                     /label=EM7\promoter
     rep_origin      2828..3256
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     rep_origin      complement(4782..5455)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     promoter        3261..3630
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     polyA_signal    4269..4399
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     primer          863..882
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     promoter        863..879
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    939..2489
                     /vntifkey="21"
                     /label=GK5_v1
BASE COUNT     1620 a      1637 c      1652 g      1687 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 tagagctcct taaggttaac tctagagaat tcggatccat gtcagggcag caggagcgtg 
      961 cagagcggca gcgagaggaa ctgagcgcgt cggcctcgcc gccctcccgc ttcgtgctgg 
     1021 ggctggacgt gggcagcacg gtgatccgct gccacgtcta tgaccagacg gcgcgagtgc 
     1081 gaggctccag cgcgcagaag gtagaaaatg tttaccctca acctggctgg gttgaaatcg 
     1141 atcctgattc tctctgggct cagtttgttg ccgtaataaa agatgctgtc aaagctgcag 
     1201 gggtgcagat gaatcaaatt gtcggtcttg gcatatcaac acagagagcc actttcatta 
     1261 catggaacaa gaaaacaggc catcattttc acaacttcat tagctggcaa gacctaaggg 
     1321 cagctgaact tgtaaagtcc tggaataatt ctctcataat gaagctattg catggggcca 
     1381 cccgagtcct tcatttcttc agtagaagta aagtaatgct aacggtcagc cgcttcaatt 
     1441 tcagcaccca gcatgccacc ttaagattga cctggatttt acaaaaccta tctgaggtga 
     1501 aaagggcagt tgaagaagac aattgttgct ttgggacgat tgatacctgg ctgttataca 
     1561 agctcacgaa aggttcttcc tacgccacgg attattcaaa tgccagcacc actggatttt 
     1621 ttgatcccta tgcgatgcgc tggagcaggc tcataaccac catggtctcc atcccactgt 
     1681 ctatcctgcc tcctgtgaag gataccagct ataattttgg atcagtggat gagaagatat 
     1741 ttggcgtgcc tataccagtt gttgccttgg ttggtgacca gcagtcggcc atgtttggag 
     1801 agtgctgctt cgagacggga gatgtgaagc taaccatggg aactggaaca tttttggata 
     1861 tcaatactgg aaaaaacctt cagcatgtta atggaggctt ttatccacta attggatgga 
     1921 agattgggca agaacttgta tgtttagctg aaggcaatgc aggagacacg ggcaccgcca 
     1981 taatgtgggc tcaaaaatta gatctcttca cagacgctgc tgagactgaa aaaatggccc 
     2041 tgagcctgga agattctgaa ggggtttact ttgttccgtc gtttagtgga ttacaggctc 
     2101 cattaaatga cccctgtgcc tgtgcctctt ttatgggttt gaagcactcc actaataaat 
     2161 accatcttgt ccgagcaata ttggagtcaa tagctttcag gaacaaacag ttgtacgaca 
     2221 tgctgcagag agaaatccag attcccgtca cgaacatccg ggcagatgga ggcgtgtgta 
     2281 acaacgcttt cgtcatgcag atgacttcag acctgataaa tgagaagata gacagacctg 
     2341 cccacttcga catgtcctgc ttgggggcag cctctctggc tggcctcgct gttggatttt 
     2401 gggcggacaa ggaagagctc cagaagctga ggcaaagcga gatggttttc aagcctcaga 
     2461 agaagtggca ggaatacgag gcattttaac tcgagccatg gaagcttgat atctaactga 
     2521 ctgaaccggt ggtaccaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca 
     2581 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 
     2641 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 
     2701 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 
     2761 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg 
     2821 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 
     2881 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 
     2941 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 
     3001 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 
     3061 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 
     3121 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 
     3181 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 
     3241 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 
     3301 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 
     3361 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 
     3421 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 
     3481 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg 
     3541 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 
     3601 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc 
     3661 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa 
     3721 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat 
     3781 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt 
     3841 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg 
     3901 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa 
     3961 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg 
     4021 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt 
     4081 gctgccctct ggttatgtgt gggagggcta agcacttcgt ggccgaggag caggactgac 
     4141 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 
     4201 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 
     4261 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 
     4321 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 
     4381 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 
     4441 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 
     4501 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 
     4561 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 
     4621 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 
     4681 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 
     4741 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 
     4801 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
     4861 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
     4921 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
     4981 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 
     5041 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
     5101 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
     5161 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
     5221 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
     5281 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
     5341 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
     5401 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
     5461 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 
     5521 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 
     5581 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 
     5641 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 
     5701 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 
     5761 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 
     5821 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 
     5881 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 
     5941 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 
     6001 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 
     6061 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 
     6121 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 
     6181 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 
     6241 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 
     6301 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 
     6361 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 
     6421 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 
     6481 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
     6541 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc 
//
