LOCUS       pcDNA6-GK5_v2           6647 bp    DNA     circular SYN 26-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007718|
COMMENT     VNTDBDATE|-13007718|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pcDNA6-GK5_v2|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Invitrogen|
COMMENT     VNTOAUTHORTEL|(800)-357-3114|
COMMENT     VNTOAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTOAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     CDS             complement(5651..6511)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     polyA_site      2609..2833
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     promoter        complement(6512..6610)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             3764..4162
                     /invitrogen="1050000"
                     /vntifkey="4"
                     /label=Bsd(R)
     primer          769..789
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     promoter        232..819
                     /invitrogen="2180000"
                     /vntifkey="29"
                     /label=CMV\promoter
     promoter        3696..3763
                     /invitrogen="2340000"
                     /vntifkey="30"
                     /label=EM7\promoter
     rep_origin      2879..3307
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     rep_origin      complement(4833..5506)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     promoter        3312..3681
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     polyA_signal    4320..4450
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     primer          863..882
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     promoter        863..879
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    939..2540
                     /vntifkey="21"
                     /label=GK5_v2
BASE COUNT     1639 a      1644 c      1669 g      1695 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 tagagctcct taaggttaac tctagagaat tcggatccat gtcagggcag caggagcgtg 
      961 cagagcggca gcgagaggaa ctgagcgcgt cggcctcgcc gccctcccgc ttcgtgctgg 
     1021 ggctggacgt gggcagcacg gtgatccgct gccacgtcta tgaccagacg gcgcgagtgc 
     1081 gaggctccag cgcgcagaag gtagaaaatg tttaccctca acctggctgg gttgaaatcg 
     1141 atcctgattc tctctgggct cagtttgttg ccgtaataaa agatgctgtc aaagctgcag 
     1201 gggtgcagat gaatcaaatt gtcggtcttg gcatatcaac acagagagcc actttcatta 
     1261 catggaacaa gaaaacaggc catcattttc acaacttcat tagctggcaa gacctaaggg 
     1321 cagctgaact tgtaaagtcc tggaataatt ctctcataat gaagctattg catggggcca 
     1381 cccgagtcct tcatttcttc agtagaagta aagtaatgct aacggtcagc cgcttcaatt 
     1441 tcagcaccca gcatgccacc ttaagattga cctggatttt acaaaaccta tctgaggtga 
     1501 aaagggcagt tgaagaagac aattgttgct ttgggacgat tgatacctgg ctgttataca 
     1561 agctcacgaa aggttcttcc tacgccacgg attattcaaa tgccagcacc actggatttt 
     1621 ttgatcccta tgcgatgcgc tggagcaggc tcataaccac catggtctcc atcccactgt 
     1681 ctatcctgcc tcctgtgaag gataccagct ataattttgg atcagtggat gagaagatat 
     1741 ttggcgtgcc tataccagtt gttgccttgg ttggtgacca gcagtcggcc atgtttggag 
     1801 agtgctgctt cgagacggga gatgtgaagc taaccatggg aactggaaca tttttggata 
     1861 tcaatactgg aaaaaacctt cagcatgtta atggaggctt ttatccacta attggatgga 
     1921 agattgggca agaacttgta tgtttagctg aaggcaatgc aggagacacg ggcaccgcca 
     1981 taatgtgggc tcaaaaatta gatctcttca cagacgctgc tgagactgaa aaaatggccc 
     2041 tgagcctgga agattctgaa ggggtttact ttgttccgtc gtttagtgga ttacaggctc 
     2101 cattaaatga cccctgtgcc tgtgcctctt ttatgggttt gaagcactcc actaataaat 
     2161 accatcttgt ccgagcaata ttggagtcaa tagctttcag gaacaaacag ttgtacgaca 
     2221 tgctgcagag agaaatccag attcccgtca cgaacatccg ggcagatgga ggcgtgtgta 
     2281 acaacgcttt cgtcatgcag atgacttcag acctgataaa tgagaagata gacagacctg 
     2341 cccacttcga catgtcctgc ttgggggcag cctctctggc tggcctcgct gttggatttt 
     2401 gggcggacaa ggaagagctc cagaagctga ggcaaagcga gatggttttc aagcctcaga 
     2461 agaagtggca ggaatacgag gtgaatatgg aaaactgggt aaaagcggtg aagcgctcca 
     2521 tgaattggta taacaagacg ctcgagccat ggaagcttga tatctaactg actgaaccgg 
     2581 tggtaccaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 
     2641 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 
     2701 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 
     2761 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 
     2821 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 
     2881 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 
     2941 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 
     3001 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 
     3061 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 
     3121 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 
     3181 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 
     3241 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 
     3301 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 
     3361 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 
     3421 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 
     3481 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     3541 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 
     3601 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 
     3661 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata 
     3721 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agcctttgtc 
     3781 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc 
     3841 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt 
     3901 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc 
     3961 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat 
     4021 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc 
     4081 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc 
     4141 tggttatgtg tgggagggct aagcacttcg tggccgagga gcaggactga cacgtgctac 
     4201 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 
     4261 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 
     4321 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 
     4381 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 
     4441 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 
     4501 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 
     4561 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
     4621 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
     4681 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
     4741 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     4801 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     4861 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     4921 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     4981 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     5041 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     5101 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     5161 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     5221 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     5281 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 
     5341 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     5401 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     5461 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     5521 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     5581 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     5641 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     5701 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
     5761 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
     5821 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
     5881 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
     5941 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
     6001 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
     6061 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
     6121 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
     6181 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
     6241 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
     6301 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
     6361 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
     6421 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
     6481 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
     6541 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
     6601 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc 
//
