LOCUS       pcDNA6-C-Flag-GK        6620 bp    DNA     circular SYN 26-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007721|
COMMENT     VNTDBDATE|-13007721|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pcDNA6-C-Flag-GK5_v1|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Invitrogen|
COMMENT     VNTOAUTHORTEL|(800)-357-3114|
COMMENT     VNTOAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTOAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     CDS             complement(5624..6484)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     polyA_site      2582..2806
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     promoter        complement(6485..6583)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             3737..4135
                     /invitrogen="1050000"
                     /vntifkey="4"
                     /label=Bsd(R)
     primer          769..789
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     promoter        232..819
                     /invitrogen="2180000"
                     /vntifkey="29"
                     /label=CMV\promoter
     promoter        3669..3736
                     /invitrogen="2340000"
                     /vntifkey="30"
                     /label=EM7\promoter
     rep_origin      2852..3280
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     rep_origin      complement(4806..5479)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     promoter        3285..3654
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     polyA_signal    4293..4423
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     primer          863..882
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     promoter        863..879
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    2505..2531
                     /vntifkey="21"
                     /label=C-Flag
     misc_feature    939..2486
                     /vntifkey="21"
                     /label=GK5_v1
BASE COUNT     1630 a      1642 c      1659 g      1689 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 tagagctcct taaggttaac tctagagaat tcggatccat gtcagggcag caggagcgtg 
      961 cagagcggca gcgagaggaa ctgagcgcgt cggcctcgcc gccctcccgc ttcgtgctgg 
     1021 ggctggacgt gggcagcacg gtgatccgct gccacgtcta tgaccagacg gcgcgagtgc 
     1081 gaggctccag cgcgcagaag gtagaaaatg tttaccctca acctggctgg gttgaaatcg 
     1141 atcctgattc tctctgggct cagtttgttg ccgtaataaa agatgctgtc aaagctgcag 
     1201 gggtgcagat gaatcaaatt gtcggtcttg gcatatcaac acagagagcc actttcatta 
     1261 catggaacaa gaaaacaggc catcattttc acaacttcat tagctggcaa gacctaaggg 
     1321 cagctgaact tgtaaagtcc tggaataatt ctctcataat gaagctattg catggggcca 
     1381 cccgagtcct tcatttcttc agtagaagta aagtaatgct aacggtcagc cgcttcaatt 
     1441 tcagcaccca gcatgccacc ttaagattga cctggatttt acaaaaccta tctgaggtga 
     1501 aaagggcagt tgaagaagac aattgttgct ttgggacgat tgatacctgg ctgttataca 
     1561 agctcacgaa aggttcttcc tacgccacgg attattcaaa tgccagcacc actggatttt 
     1621 ttgatcccta tgcgatgcgc tggagcaggc tcataaccac catggtctcc atcccactgt 
     1681 ctatcctgcc tcctgtgaag gataccagct ataattttgg atcagtggat gagaagatat 
     1741 ttggcgtgcc tataccagtt gttgccttgg ttggtgacca gcagtcggcc atgtttggag 
     1801 agtgctgctt cgagacggga gatgtgaagc taaccatggg aactggaaca tttttggata 
     1861 tcaatactgg aaaaaacctt cagcatgtta atggaggctt ttatccacta attggatgga 
     1921 agattgggca agaacttgta tgtttagctg aaggcaatgc aggagacacg ggcaccgcca 
     1981 taatgtgggc tcaaaaatta gatctcttca cagacgctgc tgagactgaa aaaatggccc 
     2041 tgagcctgga agattctgaa ggggtttact ttgttccgtc gtttagtgga ttacaggctc 
     2101 cattaaatga cccctgtgcc tgtgcctctt ttatgggttt gaagcactcc actaataaat 
     2161 accatcttgt ccgagcaata ttggagtcaa tagctttcag gaacaaacag ttgtacgaca 
     2221 tgctgcagag agaaatccag attcccgtca cgaacatccg ggcagatgga ggcgtgtgta 
     2281 acaacgcttt cgtcatgcag atgacttcag acctgataaa tgagaagata gacagacctg 
     2341 cccacttcga catgtcctgc ttgggggcag cctctctggc tggcctcgct gttggatttt 
     2401 gggcggacaa ggaagagctc cagaagctga ggcaaagcga gatggttttc aagcctcaga 
     2461 agaagtggca ggaatacgag gcatttctcg agccatggaa gcttatggac tacaaagacg 
     2521 atgacgacaa ggatatctaa ctgactgaac cggtggtacc aaacccgctg atcagcctcg 
     2581 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 
     2641 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 
     2701 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 
     2761 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 
     2821 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 
     2881 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 
     2941 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 
     3001 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 
     3061 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 
     3121 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 
     3181 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 
     3241 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 
     3301 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 
     3361 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 
     3421 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 
     3481 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     3541 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt 
     3601 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg 
     3661 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa 
     3721 ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag 
     3781 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc 
     3841 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc 
     3901 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg 
     3961 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca 
     4021 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc 
     4081 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagcact 
     4141 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct 
     4201 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 
     4261 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt 
     4321 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     4381 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta 
     4441 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 
     4501 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 
     4561 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 
     4621 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 
     4681 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 
     4741 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 
     4801 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
     4861 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
     4921 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
     4981 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
     5041 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 
     5101 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     5161 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     5221 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     5281 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 
     5341 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
     5401 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
     5461 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
     5521 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
     5581 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
     5641 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
     5701 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
     5761 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
     5821 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
     5881 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 
     5941 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
     6001 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
     6061 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
     6121 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
     6181 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
     6241 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
     6301 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
     6361 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
     6421 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
     6481 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 
     6541 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 
     6601 gaaaagtgcc acctgacgtc 
//
