LOCUS       pcDNA6-N-Flag-SR        8471 bp    DNA     circular SYN 26-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007740|
COMMENT     VNTDBDATE|-13007740|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pcDNA6-N-Flag-SREBP2|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Invitrogen|
COMMENT     VNTOAUTHORTEL|(800)-357-3114|
COMMENT     VNTOAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTOAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     CDS             complement(7475..8335)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     polyA_site      4433..4657
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     promoter        complement(8336..8434)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             5588..5986
                     /invitrogen="1050000"
                     /vntifkey="4"
                     /label=Bsd(R)
     primer          769..789
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     promoter        232..819
                     /invitrogen="2180000"
                     /vntifkey="29"
                     /label=CMV\promoter
     promoter        5520..5587
                     /invitrogen="2340000"
                     /vntifkey="30"
                     /label=EM7\promoter
     rep_origin      4703..5131
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     rep_origin      complement(6657..7330)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     promoter        5136..5505
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     polyA_signal    6144..6274
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     primer          863..882
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     promoter        863..879
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    927..953
                     /vntifkey="21"
                     /label=N-Flag
     misc_feature    972..4364
                     /vntifkey="21"
                     /label=SREBP2
BASE COUNT     1940 a      2337 c      2203 g      1991 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 tagagctcct taaggttaac gccaccatgg actacaaaga cgatgacgac aagtctagag 
      961 aattcggatc catggatgag agcagcgagc tgggcgttct ggagaccatg gagaccctca 
     1021 cggagctggg cgatgagctg actctcgggg acatcgacga gatgctacag tttgtcagca 
     1081 atcaagtggg agagttccct gatttgtttt cagagcaact ttgtagctcc ttccctggag 
     1141 gtggcagcaa tggtggcagt ggcaacaaca gcagtggcag aggcaacaat ggtggagcca 
     1201 cagaccctgc agtacagcgg tcattcagcc aggtcccatt atctaccttc tctccctcag 
     1261 ctgcctcccc tcaggctccg gccctgcagg tcaaagtctc tcctacccca ccaagggcaa 
     1321 cccctgttct ccagccgcga ccccagcccc agccccaacc tccagctcag ctgcagcagc 
     1381 agacggtgat gatcaccccg acgttcagca ccgctccgca gacgaggatc atccagcagc 
     1441 ctttgatata ccagaacgca gcgaccagct ttcaagtcct gcagcctcaa gtgcaaagcc 
     1501 tcgtgacgtc cccgcaggtg cagcccgtca ccatccagca gcaggtgcag acggtacagg 
     1561 cccagcgggt gttgacgcag acagccaatg gcacactgca gacccttgcc cccgccacgg 
     1621 tacagacggt cgctgcgcca caggtgcagc aggtcccggt cctggtgcaa cctcagatca 
     1681 ttaagacaga ttcccttgtg ttgaccacgc tgaagacaga tggcagccct gtcatggctg 
     1741 cagtccagaa cccggccctc acggccctca ctgcccccat ccagacagcc gcccttcaag 
     1801 tgccaaccct ggtgggcagc aacgggacca ttctgaccac aatgcctgtg atgatggggc 
     1861 aagagaaagt tcctatcaag caagtgcctg gcggcgtgaa gcagctggat cctcccaaag 
     1921 aaggagagag gcggacaaca cacaatatca ttgaaaagcg ctaccggtcc tccatcaacg 
     1981 acaaaatcat agagttgaag gacttagtca tggggacaga tgccaagatg cacaagtctg 
     2041 gcgttctgag gaaggccatt gattacatca aatatctgca gcaggtcaat cacaagctgc 
     2101 gccaggagaa catggtgctg aagctggcca atcagaaaaa caagctcctg aagggcatcg 
     2161 acctgggcag tctggtggac agtgatgtgg acttgaaaat tgatgacttt aaccagaatg 
     2221 tccttctgat gtctccgccg gcctccgact ccgggtccca ggccggcttc tctccctatt 
     2281 ccattgactc tgagccgggc agccctctgc tggatgacgc aaaggtcaag gatgaaccgg 
     2341 actctcctcc tgtggcactg ggcatggtgg accgctctcg aatcctctta tgtgtcctca 
     2401 ccttcctggg cctctccttt aaccccttga cttccttgct gcagtgggga ggggcccaca 
     2461 acactgacca gcacccatac tcaggctcgg gccgcagtgt actgtcactg gagtcaggtg 
     2521 ctgggggctg gtttgactgg atggttccaa ctctcctcct gtggctggta aatggtgtga 
     2581 ttgtcttgag cgtctttgtg aagctgttgg tccacgggga gccggtgatt cgcccacact 
     2641 cacgcccctc agtcaccttc tggagacacc ggaagcaggc agacctagac ctcgccaaag 
     2701 gtgatttcgc agctgctgct gccaacctac aaacctgctt atccgtgctg ggccgggcgc 
     2761 tgcccacctc ccgcctggac ttggcctgca gcctctcctg gaatgtgatc cgctacagcc 
     2821 tgcagaagct gcgcctggta cgctggttac tcaagaaggt cttccagcgc tggcgggcta 
     2881 ctcccgccac tgcagctggc tttgaggatg aggctaagag cagcgcaagg gatgcggcac 
     2941 tggcctacca caggctgcac cagctgcaca tcacagggaa gcttccggcg ggatctgcct 
     3001 gttcagatgt gcacatggct ctgtgtgctg tgaacctggc cgagtgtgcg gaggagaaaa 
     3061 tcctaccaag cacactgatt gagatccacc tgacagcggc catggggctc aagacccgct 
     3121 gtggaggcaa gctgggcttc ttggctagct acttcttaaa tcgtgcccag agtctgtgtg 
     3181 gcccagagca tagcactgtc cccgactccc tgcgctggct atgccatccc ttgggccaga 
     3241 agtttttcat ggagcgaagc tggtcgatca agtcagcagc caaggagagc ctgtactgcg 
     3301 cccagaggag cccagctgac cccattgccc aggtacacca ggcgttctgc aaaaacctgc 
     3361 tagaaagagc ggtggagtcc ttggtgaaac ctcaggccaa gaagaaggca ggcgaccagg 
     3421 aagaagagag ctgtgaattt tccagtgctc tagagtattt gaaattactt cattcttttg 
     3481 tggactctgt gggatttgtg acctcaccat tctccagcag ttccgtgctc aggtcggccc 
     3541 ttggccccga tgtcatctgc cggtggtgga cgtccgcagt caccatggcc atcagctggc 
     3601 tccagggaga cgatgcagct gtgcgctctc gttttactga agtagagcgg gtccccaagg 
     3661 ccctggaagt gaccgagagt cccttggtga aggccgtctt ctatacctgt agagccatgc 
     3721 atgcctccct ctctgggaaa gctgacgggc agcagaactc cttctgtcac tgtgagaggg 
     3781 ccagtggtca cctgtggagc agtctcaacg tcagcggcac tacttctgat ccttcactta 
     3841 accatgtgat ccagctgttc acctgtgacc tgctactgtc gctgaggaca gcactctggc 
     3901 aaaagcaggc cagtgccagc cagctcctgg gtgagaccta ccatgcatcc ggcactgagc 
     3961 tggccggctt ccagcgggac ctgggcagcc ttcgaaggct ggcccatagc ttccgcccag 
     4021 cctaccgcaa ggtgttcctg catgaagcca ctgtgcgcct gatggcagga gccagcccta 
     4081 cccgtacaca ccagctgctg gagcatagcc tacggcggcg ccccactcag aacaccaagc 
     4141 atggagaggt agacacctgg cctggccaac gagagcgagc tactgccatc ctgctggcct 
     4201 gccgccatct tcccctctct ttcctgtctt ccccgggcca gcgggcagtg ctgctggctg 
     4261 aagctgcccg caccctggag aaggtgggtg accgccgttc ctgcagtgac tgccagcaga 
     4321 tgattgtcaa gctgggcggt ggcactgcca tcgctgcctc ctgactcgag ccatggaagc 
     4381 ttgatatcta actgactgaa ccggtggtac caaacccgct gatcagcctc gactgtgcct 
     4441 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt 
     4501 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg 
     4561 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac 
     4621 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc 
     4681 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 
     4741 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 
     4801 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 
     4861 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 
     4921 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 
     4981 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 
     5041 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 
     5101 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt 
     5161 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 
     5221 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 
     5281 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 
     5341 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 
     5401 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 
     5461 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg 
     5521 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa 
     5581 ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc 
     5641 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag 
     5701 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga 
     5761 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc 
     5821 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct 
     5881 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt 
     5941 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac ttcgtggccg 
     6001 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt 
     6061 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 
     6121 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa 
     6181 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 
     6241 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct 
     6301 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 
     6361 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 
     6421 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 
     6481 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 
     6541 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
     6601 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
     6661 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     6721 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     6781 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     6841 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     6901 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     6961 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     7021 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     7081 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     7141 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     7201 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     7261 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     7321 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     7381 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     7441 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     7501 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     7561 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 
     7621 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     7681 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     7741 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 
     7801 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     7861 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     7921 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     7981 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     8041 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
     8101 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     8161 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     8221 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     8281 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     8341 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     8401 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     8461 cacctgacgt c 
//
