LOCUS       pMBP-parallel1-G        8306 bp    DNA     circular SYN 26-SEP-2019
DEFINITION  Expression vector pMBP-parallel1, complete sequence.
ACCESSION   AF097412
VERSION     AF097412.1  GI:3983120
SOURCE      Expression vector pMBP-parallel1.
  ORGANISM  Expression vector pMBP-parallel1
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Sheffield,P., Garrard,S. and Derewenda,Z.
  TITLE     Overcoming expression and purification problems of RhoGDI using a
            family of 'parallel' expression vectors
  JOURNAL   Protein Expr. Purif. 15 (1), 34-39 (1999)
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Sheffield,P.J., Garrard,S.M. and Derewenda,Z.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-1998) Molecular Physiology and Biological
            Physics, University of Virginia, 4215 Jordan Hall, 1300 Jefferson
            Park Avenue, Charlottesville, Virginia 22908, USA
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13007756|
COMMENT     VNTDBDATE|-13007756|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMBP-parallel1-GK5_v2 (D30N)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|MZ|
FEATURES             Location/Qualifiers
     gene            204..1163
                     /gene="lacIq"
                     /vntifkey="60"
     CDS             204..1163
                     /gene="lacIq"
                     /codon_start=1
                     /transl_table=11
                     /product="lac repressor"
                     /protein_id="AAC83812.1"
                     /db_xref="GI:3983121"
                     /vntifkey="4"
                     /label=lacIq
     promoter        1453..1481
                     /vntifkey="30"
                     /label=Promoter_P_1
                     /note="tac; inducible promoter"
     misc_feature    2632..2688
                     /gene="malE"
                     /vntifkey="21"
                     /label=Protein\spacer\region
                     /note="protein spacer region"
     misc_feature    2689..2709
                     /gene="malE"
                     /vntifkey="21"
                     /label=TEV
                     /note="Tobacco etch virus protease cleavage site"
     gene            5008..5938
                     /gene="bla"
                     /vntifkey="60"
     promoter        5008..5036
                     /gene="bla"
                     /vntifkey="30"
                     /label=bla
     CDS             5078..5938
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAC83814.1"
                     /db_xref="GI:3983123"
                     /vntifkey="4"
                     /label=AMPr
     misc_feature    1528..2631
                     /vntifkey="21"
                     /label=MBP
                     /note="maltose binding protein (MBP)"
     misc_feature    2728..4332
                     /vntifkey="21"
                     /label=GK5_v2\(D30N)
BASE COUNT     2135 a      2047 c      2203 g      1921 t 
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg 
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg 
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt 
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga 
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga 
     1501 gcacttcacc aacaaggacc atagattatg aaaatcgaag aaggtaaact ggtaatctgg 
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat 
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt 
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac 
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat 
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt 
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa 
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac 
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag 
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg 
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac 
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg 
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc 
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt 
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg 
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag 
     2461 ttggcgaaag atccacgtat tgccgccaca atggaaaacg cccagaaagg tgaaatcatg 
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc 
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg 
     2641 aacaacaaca acaataacaa taacaacaac ctcgggatcg atacgaccga aaacctgtat 
     2701 tttcagggcg ccatggatcc ggaattcatg tcagggcagc aggagcgtgc agagcggcag 
     2761 cgagaggaac tgagcgcgtc ggcctcgccg ccctcccgct tcgtgctggg gctgaacgtg 
     2821 ggcagcacgg tgatccgctg ccacgtctat gaccagacgg cgcgagtgcg aggctccagc 
     2881 gcgcagaagg tagaaaatgt ttaccctcaa cctggctggg ttgaaatcga tcctgattct 
     2941 ctctgggctc agtttgttgc cgtaataaaa gatgctgtca aagctgcagg ggtgcagatg 
     3001 aatcaaattg tcggtcttgg catatcaaca cagagagcca ctttcattac atggaacaag 
     3061 aaaacaggcc atcattttca caacttcatt agctggcaag acctaagggc agctgaactt 
     3121 gtaaagtcct ggaataattc tctcataatg aagctattgc atggggccac ccgagtcctt 
     3181 catttcttca gtagaagtaa agtaatgcta acggtcagcc gcttcaattt cagcacccag 
     3241 catgccacct taagattgac ctggatttta caaaacctat ctgaggtgaa aagggcagtt 
     3301 gaagaagaca attgttgctt tgggacgatt gatacctggc tgttatacaa gctcacgaaa 
     3361 ggttcttcct acgccacgga ttattcaaat gccagcacca ctggattttt tgatccctat 
     3421 gcgatgcgct ggagcaggct cataaccacc atggtctcca tcccactgtc tatcctgcct 
     3481 cctgtgaagg ataccagcta taattttgga tcagtggatg agaagatatt tggcgtgcct 
     3541 ataccagttg ttgccttggt tggtgaccag cagtcggcca tgtttggaga gtgctgcttc 
     3601 gagacgggag atgtgaagct aaccatggga actggaacat ttttggatat caatactgga 
     3661 aaaaaccttc agcatgttaa tggaggcttt tatccactaa ttggatggaa gattgggcaa 
     3721 gaacttgtat gtttagctga aggcaatgca ggagacacgg gcaccgccat aatgtgggct 
     3781 caaaaattag atctcttcac agacgctgct gagactgaaa aaatggccct gagcctggaa 
     3841 gattctgaag gggtttactt tgttccgtcg tttagtggat tacaggctcc attaaatgac 
     3901 ccctgtgcct gtgcctcttt tatgggtttg aagcactcca ctaataaata ccatcttgtc 
     3961 cgagcaatat tggagtcaat agctttcagg aacaaacagt tgtacgacat gctgcagaga 
     4021 gaaatccaga ttcccgtcac gaacatccgg gcagatggag gcgtgtgtaa caacgctttc 
     4081 gtcatgcaga tgacttcaga cctgataaat gagaagatag acagacctgc ccacttcgac 
     4141 atgtcctgct tgggggcagc ctctctggct ggcctcgctg ttggattttg ggcggacaag 
     4201 gaagagctcc agaagctgag gcaaagcgag atggttttca agcctcagaa gaagtggcag 
     4261 gaatacgagg tgaatatgga aaactgggta aaagcggtga agcgctccat gaattggtat 
     4321 aacaagacgt agactagtgc ggccgctttc gaatctagag cctgcagtct cgaggcatgc 
     4381 ggtaccaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt 
     4441 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga 
     4501 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcagcttgg 
     4561 ctgttttggc ggatgagata agattttcag cctgatacag attaaatcag aacgcagaag 
     4621 cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat 
     4681 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag 
     4741 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 
     4801 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg 
     4861 atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg 
     4921 ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa 
     4981 ctctttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 
     5041 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 
     5101 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 
     5161 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 
     5221 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttctc caatgatgag 
     5281 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca 
     5341 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 
     5401 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 
     5461 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 
     5521 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 
     5581 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 
     5641 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 
     5701 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 
     5761 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 
     5821 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 
     5881 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 
     5941 gtcagaccaa gtttactcat atatacttta gattgattta ccccggttga taatcagaaa 
     6001 agccccaaaa acaggaagat tgtataagca aatatttaaa ttgtaaacgt taatattttg 
     6061 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 
     6121 ggcaaaatcc cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt 
     6181 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 
     6241 tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg 
     6301 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 
     6361 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 
     6421 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 
     6481 ctacagggcg cgtaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 
     6541 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 
     6601 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 
     6661 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 
     6721 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 
     6781 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 
     6841 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 
     6901 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 
     6961 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 
     7021 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 
     7081 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 
     7141 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 
     7201 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 
     7261 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 
     7321 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct 
     7381 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct 
     7441 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt 
     7501 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 
     7561 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 
     7621 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg 
     7681 tggtcgtgca gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc 
     7741 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc 
     7801 tgtttggtca cttgatgcct ccgtgtaagg gggaatttct gttcatgggg gtaatgatac 
     7861 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac 
     7921 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca 
     7981 ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc 
     8041 agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca 
     8101 gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt 
     8161 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa 
     8221 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg 
     8281 gccaggaccc aacgctgccc gaaatt 
//
