LOCUS       Exported                3750 bp ds-DNA     circular SYN 04-FEB-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      403..412
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             736..756
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             765..782
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    811..1035
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1106..1122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1130..1146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1154..1184)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1199..1220
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1508..2096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2267..3127)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3128..3232)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(3502..3750,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gggcaagatc
      421 agcgtggaca gcgagaagct gctgaacgag gccatcaacg acttcgacat cttcggcgag
      481 gacttcaacg tgtacgccat ctacagctac agagaggact acgacttcga gtacatcagc
      541 gactacgtgg acgccgacga gcccaccaga gacgagttcg agaccgagga ggactaccag
      601 gaggtgatga aggacttcaa ggagaacctg gacagcctga agttcaccaa gcacaagaag
      661 atgaccatcg ccgacctggt gcacgagctg tgggagcaga acagaatctt cggatccggc
      721 ggtggaggca gtgggcccaa gaagaagagg aaagtctgac cggtcatcat caccatcacc
      781 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
      841 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
      901 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
      961 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     1021 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgata ccgtcgacct
     1081 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1141 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctag ggtgcctaat
     1201 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1261 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1321 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1381 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1441 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1501 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1561 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1621 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1681 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     1741 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1801 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1861 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1921 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     1981 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2041 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2101 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2161 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2221 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2281 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2341 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2401 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2461 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2521 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2581 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2641 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2701 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2761 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2821 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2881 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2941 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3001 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3061 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3121 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3181 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3241 cccgaaaagt gccacctgac gtcgacggat cgggagatcg atctcccgat cccctagggt
     3301 cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     3361 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     3421 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     3481 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     3541 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     3601 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     3661 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggactat ttacggtaaa
     3721 ctgcccactt ggcagtacat caagtgtatc
//
