LOCUS       Exported                4059 bp ds-DNA     circular SYN 04-FEB-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      403..412
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1045..1065
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1074..1091
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1120..1344
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1415..1431)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1439..1455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1463..1493)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1508..1529
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1817..2405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2576..3436)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3437..3541)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(3811..4059,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gggctacgtg
      421 ggcaagagca gaagcgtgag aagccagatc gccatcgaca acgccgaggt gcccctgaac
      481 cacatcacca aggactacat cctgaccttc gtgaccgaga acaacatcga cgagaccctg
      541 aagaacgaga gcgtggccat gtggaagttc gtggccaaga gacacggcag caccagctgg
      601 caccacgtga gcaagcacta caacaagatc gaccactacg acctgcacga cgtggccgag
      661 tacttcagca tgaactacga cagcctgaag aacgactacc agaacctgct ggaccagaag
      721 agacaggcca agaacgacct gatcaagaac ctgaagctgg gcatcatcaa ggtgcagatc
      781 tggggcggca ccaagagata ccccaagctg gagggctacg agagcgtgat gggcgtggtg
      841 aaggacggct ggctgcacac cgtgaccctg agcaaccaga ccaagtacaa gatcaccggc
      901 aacaagatcg aggagatcac catcttcgag ctggaccagt acgacatcct gaccaagaag
      961 ttccccgagt tcagagccat gaagagaaag atcaacaagg aggtggccag actgagcaag
     1021 ggatccggcg gtggaggcag tgggcccaag aagaagagga aagtctgacc ggtcatcatc
     1081 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     1141 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     1201 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     1261 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     1321 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac
     1381 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     1441 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg
     1501 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     1561 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1981 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     2041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2221 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     2281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2341 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2401 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2461 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2521 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2581 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2641 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2701 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2761 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2821 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2881 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2941 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3001 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3061 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3121 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3181 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3241 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3301 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3361 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3421 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3481 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3541 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc
     3601 ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     3661 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     3721 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     3781 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     3841 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     3901 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     3961 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggactatt
     4021 tacggtaaac tgcccacttg gcagtacatc aagtgtatc
//
