LOCUS       Exported                3723 bp ds-DNA     circular SYN 04-FEB-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      403..412
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             709..729
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             738..755
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    784..1008
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1079..1095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1103..1119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1127..1157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1172..1193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1481..2069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2240..3100)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3101..3205)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(3475..3723,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gggcaaggag
      421 gtgttcaagc tgaagcccga gctggtgacc tacaagggct gcggctgggc cctggcctgc
      481 atcaaggacg gcgagatcat cgacctgacc tacgtgagag acctgggcat cgaggagtac
      541 gacgagaact tcgacggcct ggagcccgag atcatctact acgacgtggt ggccagccag
      601 gcctgcaagg aggtggccta cagatacgag gagatgggcg agttcacctt cggcctgtgc
      661 agctgctggg agttcaacgt gatgggatcc ggcggtggag gcagtgggcc caagaagaag
      721 aggaaagtct gaccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
      781 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
      841 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
      901 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
      961 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     1021 aaagaaccag ctggggctcg ataccgtcga cctctagcta gagcttggcg taatcatggt
     1081 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     1141 gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca ttaattgcgt
     1201 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     1261 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     1321 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1381 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1441 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1501 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1561 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1621 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     1681 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1741 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1801 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1861 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1921 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1981 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2041 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2101 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2161 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2221 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2281 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2341 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2401 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2461 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2521 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2581 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2641 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2701 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2761 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2821 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2881 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2941 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3001 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3061 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3121 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3181 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     3241 gatcgggaga tcgatctccc gatcccctag ggtcgactct cagtacaatc tgctctgatg
     3301 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     3361 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     3421 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     3481 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     3541 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     3601 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     3661 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
     3721 atc
//
