LOCUS       Exported                7176 bp ds-DNA     circular SYN 01-OCT-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    HP_p15A-dG3-dPS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2019 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            complement(579..1522)
                     /label=Kanamycin resistance + Promoter (BBa_K1424003)
     gene            602..1417
                     /label=Kanamycin resistance
     oriT            join(1554..7176,1..172)
                     /direction=RIGHT
                     /label=M13 ori fragment AB
     promoter        1649..1696
                     /label=Promoter g2 BBa_M13102
     misc_feature    1713..1728
                     /label=RBS g2 BBa_M13502
     gene            1727..2961
                     /label=Gene II
     misc_feature    2511..2558
                     /label=Promoter gX BBa_M13110
     misc_feature    2610..2625
                     /label=RBS g10 BBa_M13510
     gene            2626..2961
                     /label=Gene X
     misc_feature    2916..2965
                     /label=Promoter g5 BBa_M13105
     misc_feature    2957..2972
                     /label=RBS g5 BBa_M13505
     gene            2973..3236
                     /label=Gene V
     misc_feature    3222..3237
                     /label=RBS g7 BBa_M13507
     gene            3238..3339
                     /label=Gene VII
     misc_feature    3285..3331
                     /label=Promoter g8 BBa_M13108
     misc_feature    3320..3335
                     /label=RBS g9 BBa_M13509
     gene            3336..3434
                     /label=Gene IX
     misc_feature    3415..3430
                     /label=RBS g8 BBa_M13508
     gene            3431..3652
                     /label=Gene VIII
     misc_feature    3630..3677
                     /label=Promoter g3 BBa_M13103
     misc_feature    3693..3708
                     /label=RBS g3 BBa_M13503
     gene            3789..4127
                     /label=Gene VI
     misc_feature    4019..4065
                     /label=Promoter g1 BBa_M13101
     misc_feature    4113..4128
                     /label=RBS g1 BBa_M13501
     gene            4129..5175
                     /label=Gene I
     misc_feature    4834..4848
                     /label=RBS g11 BBa_M13511
     gene            4849..5175
                     /label=Gene XI
     misc_feature    4988..5036
                     /label=Promoter g4 BBa_M13104
     misc_feature    5137..5152
                     /label=RBS g4 BBa_M13504
     gene            5153..6433
                     /label=Gene IV
     primer_bind     6233..6251
                     /label=115-F
     misc_feature    6434..6467
                     /label=BBa_B1002 Terminator
     terminator      6434..6467
                     /label=TT BBa_B1002
     primer_bind     complement(6646..6665)
                     /label=116-R
     misc_feature    complement(join(6683..7176,1..52))
                     /label=p15A ori
ORIGIN
        1 ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca
       61 tcttattaag gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
      121 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
      181 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
      241 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
      301 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
      361 cccacgctca ccggctccag atttatcagc aataaaccag ccagccgatt cgagctcgcc
      421 ccggggatcg accagttggt gattttgaac ttttgctttg ccacggaacg gtctgcgttg
      481 tcgggaagat gcgtgatctg atccttcaac tcagcaaaag ttcgatttat tcaacaaagc
      541 cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta caaccaatta accaattctg
      601 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
      661 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
      721 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
      781 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
      841 ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc
      901 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
      961 gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     1021 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     1081 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
     1141 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     1201 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     1261 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
     1321 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     1381 gcctcgagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     1441 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     1501 agagattttg agacacaacg tggctttccc cccccccccc tgcaggtctc gggctattct
     1561 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     1621 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttaaat atttgcttat
     1681 acaatcttcc tgtttttggg gattttctga ttatcaaccg gggtacatgt gattgacatg
     1741 ctagttttac gattaccgtt catcgattct cttgtttgct ccagactctc aggcaatgac
     1801 ctgatagcct ttgtagacct ctcaaaaata gctaccctct ccggcatgaa tttatcagct
     1861 agaacggttg aatatcatat tgatggtgat ttgactgtct ccggcctttc tcaccctttt
     1921 gaatctttac ctacacatta ctcaggcatt gcatttaaaa tatatgaggg ttctaaaaat
     1981 ttttatcctt gcgttgaaat aaaggcttct cccgcaaaag tattacaggg tcataatgtt
     2041 tttggtacaa ccgatttagc tttatgctct gaggctttat tgcttaattt tgctaattct
     2101 ttgccttgcc tgtatgattt attggatgtt aacgctacta ctattagtag aattgatgcc
     2161 accttttcag ctcgcgcccc aaatgaaaat atagctaaac aggttattga ccatttgcga
     2221 aatgtatcta atggtcaaac taaatctact cgttcgcaga attgggaatc aactgttaca
     2281 tggaatgaaa cttccagaca ccgtacttta gttgcatatt taaaacatgt tgagctacag
     2341 caccagattc agcaattaag ctctaagcca tccgcaaaaa tgacctctta tcaaaaggag
     2401 caattaaagg tactctctaa tcctgacctg ttggagtttg cttccggtct ggttcgcttt
     2461 gaagctcgaa ttaaaacgcg atatttgaag tctttcgggc ttcctcttaa tctttttgat
     2521 gcaatccgct ttgcttctga ctataatagt cagggtaaag acctgatttt tgatttatgg
     2581 tcattctcgt tttctgaact gtttaaagca tttgaggggg attcaatgaa tatttatgac
     2641 gattccgcag tattggacgc tatccagtct aaacatttta ctattacccc ctctggcaaa
     2701 acttcttttg caaaagcctc tcgctatttt ggtttttatc gtcgtctggt aaacgagggt
     2761 tatgatagtg ttgctcttac tatgcctcgt aattcctttt ggcgttatgt atctgcatta
     2821 gttgaatgtg gtattcctaa atctcaactg atgaatcttt ctacctgtaa taatgttgtt
     2881 ccgttagttc gttttattaa cgtagatttt tcttcccaac gtcctgactg gtataatgag
     2941 ccagttctta aaatcgcata aggtaattca caatgattaa agttgaaatt aaaccatctc
     3001 aagcccaatt tactactcgt tctggtgttt ctcgtcaggg caagccttat tcactgaatg
     3061 agcagctttg ttacgttgat ttgggtaatg aatatccggt tcttgtcaag attactcttg
     3121 atgaaggtca gccagcctat gcgcctggtc tgtacaccgt tcatctgtcc tctttcaaag
     3181 ttggtcagtt cggttccctt atgattgacc gtctgcgcct cgttccggct aagtaacatg
     3241 gagcaggtcg cggatttcga cacaatttat caggcgatga tacaaatctc cgttgtactt
     3301 tgtttcgcgc ttggtataat cgctgggggt caaagatgag tgttttagtg tattctttcg
     3361 cctctttcgt tttaggttgg tgccttcgta gtggcattac gtattttacc cgtttaatgg
     3421 aaacttcctc atgaaaaagt ctttagccct caaagcctct gtagccgttg ctaccctcgt
     3481 tccgatgctg tctttcgctg ctgagggtga cgatcccgca aaagcggcct ttaactccct
     3541 gcaagcctca gcgaccgaat atatcggtta tgcgtgggcg atggttgttg tcattgtcgg
     3601 cgcaactatc ggtatcaagc tgtttaagaa attcacctcg aaagcaagct gataaaccga
     3661 tacaattaaa ggctcctttt ggagcctttt tttttggaga ttttcaacgt gaaaaaatta
     3721 ttattcgcaa ttcctttagt tgttcctttc tattctcact ccgctgaaac tgttgaagga
     3781 tcctaatcat gccagttctt ttgggtattc cgttattatt gcgtttcctc ggtttccttc
     3841 tggtaacttt gttcggctat ctgcttactt ttcttaaaaa gggcttcggt aagatagcta
     3901 ttgctatttc attgtttctt gctcttatta ttgggcttaa ctcaattctt gtgggttatc
     3961 tctctgatat tagcgctcaa ttaccctctg actttgttca gggtgttcag ttaattctcc
     4021 cgtctaatgc gcttccctgt ttttatgtta ttctctctgt aaaggctgct attttcattt
     4081 ttgacgttaa acaaaaaatc gtttcttatt tggattggga taaataatat ggctgtttat
     4141 tttgtaactg gcaaattagg ctctggaaag acgctcgtta gcgttggtaa gattcaggat
     4201 aaaattgtag ctgggtgcaa aatagcaact aatcttgatt taaggcttca aaacctcccg
     4261 caagtcggga ggttcgctaa aacgcctcgc gttcttagaa taccggataa gccttctata
     4321 tctgatttgc ttgctattgg gcgcggtaat gattcctacg atgaaaataa aaacggcttg
     4381 cttgttctcg atgagtgcgg tacttggttt aatacccgtt cttggaatga taaggaaaga
     4441 cagccgatta ttgattggtt tctacatgct cgtaaattag gatgggatat tatttttctt
     4501 gttcaggact tatctattgt tgataaacag gcgcgttctg cattagctga acatgttgtt
     4561 tattgtcgtc gtctggacag aattacttta ccttttgtcg gtactttata ttctcttatt
     4621 actggctcga aaatgcctct gcctaaatta catgttggcg ttgttaaata tggcgattct
     4681 caattaagcc ctactgttga gcgttggctt tatactggta agaatttgta taacgcatat
     4741 gatactaaac aggctttttc tagtaattat gattccggtg tttattctta tttaacgcct
     4801 tatttatcac acggtcggta tttcaaacca ttaaatttag gtcagaagat gaaattaact
     4861 aaaatatatt tgaaaaagtt ttctcgcgtt ctttgtcttg cgattggatt tgcatcagca
     4921 tttacatata gttatataac ccaacctaag ccggaggtta aaaaggtagt ctctcagacc
     4981 tatgattttg ataaattcac tattgactct tctcagcgtc ttaatctaag ctatcgctat
     5041 gttttcaagg attctaaggg aaaattaatt aatagcgacg atttacagaa gcaaggttat
     5101 tcactcacat atattgattt atgtactgtt tccattaaaa aaggtaattc aaatgaaatt
     5161 gttaaatgta attaattttg ttttcttgat gtttgtttca tcatcttctt ttgctcaggt
     5221 aattgaaatg aataattcgc ctctgcgcga ttttgtaact tggtattcaa agcaatcagg
     5281 cgaatccgtt attgtttctc ccgatgtaaa aggtactgtt actgtatatt catctgacgt
     5341 taaacctgaa aatctacgca atttctttat ttctgtttta cgtgcaaata attttgatat
     5401 ggtaggttct aacccttcca ttattcagaa gtataatcca aacaatcagg attatattga
     5461 tgaattgcca tcatctgata atcaggaata tgatgataat tccgctcctt ctggtggttt
     5521 ctttgttccg caaaatgata atgttactca aacttttaaa attaataacg ttcgggcaaa
     5581 ggatttaata cgagttgtcg aattgtttgt aaagtctaat acttctaaat cctcaaatgt
     5641 attatctatt gacggctcta atctattagt tgttagtgct cctaaagata ttttagataa
     5701 ccttcctcaa ttcctttcaa ctgttgattt gccaactgac cagatattga ttgagggttt
     5761 gatatttgag gttcagcaag gtgatgcttt agatttttca tttgctgctg gctctcagcg
     5821 tggcactgtt gcaggcggtg ttaatactga ccgcctcacc tctgttttat cttctgctgg
     5881 tggttcgttc ggtattttta atggcgatgt tttagggcta tcagttcgcg cattaaagac
     5941 taatagccat tcaaaaatat tgtctgtgcc acgtattctt acgctttcag gtcagaaggg
     6001 ttctatctct gttggccaga atgtcccttt tattactggt cgtgtgactg gtgaatctgc
     6061 caatgtaaat aatccatttc agacgattga gcgtcaaaat gtaggtattt ccatgagcgt
     6121 ttttcctgtt gcaatggctg gcggtaatat tgttctggat attaccagca aggccgatag
     6181 tttgagttct tctactcagg caagtgatgt tattactaat caaagaagta ttgctacaac
     6241 ggttaatttg cgtgatggac agactctttt actcggtggc ctcactgatt ataaaaacac
     6301 ttctcaggat tctggcgtac cgttcctgtc taaaatccct ttaatcggcc tcctgtttag
     6361 ctcccgctct gattctaacg aggaaagcac gttatacgtg ctcgtcaaag caaccatagt
     6421 acgcgccctg tagcgcaaaa aaccccgctt cggcggggtt ttttcgcacg gatcgcttca
     6481 tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat
     6541 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg
     6601 gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg
     6661 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
     6721 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6781 cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     6841 cgctgttatg gctttgaacg ggccaaacaa cgtttcaacc cgtacgaccg agcgcccgta
     6901 aagcacccag agcatcatac atatagaacg ttctagcggc agaacgcgct agcgaccgct
     6961 cgtccgcccg tgctcaagaa caaccaagag agtttcacaa caacctaaca cccggccgtg
     7021 cgcgcgttcc ttctagcgcc ctcgcgatcg ttcgtacgtt ctagaagcaa ctagatatga
     7081 atcacgagat aacgcacgat tttcacaaca acctaacacc cggccgtgcg cgcgttcctt
     7141 cgcgctctcg cacgatcgtt cgtacgttct agaagc
//
