LOCUS       pLit_-cro_TS-J23106-g3      4325 bp    DNA     circular UNA 11-Jun-2014
DEFINITION  
FEATURES             Location/Qualifiers
     primer          complement(82..106)
                     /label="5-R"
     gene            143..1003
                     /label="Beta-lactamase"
     primer          complement(145..164)
                     /label="408-R"
     primer          910..928
                     /label="79-F"
     primer          complement(1006..1026)
                     /label="519-R"
     primer          complement(1008..1026)
                     /label="72-R"
     promoter        1027..1061
                     /label="BBa_J23106"
     primer          1027..1044
                     /label="449-F"
     primer          complement(1049..1067)
                     /label="92-R"
     RBS             1068..1079
                     /label="BBa_B0034"
     mutation        1073
                     /label="Mutation G->T"
     gene            1086..2360
                     /label="Gene III"
     primer          1086..1110
                     /label="520-F"
     terminator      2367..2495
                     /label="BBa_B0015"
     primer          2496..2517
                     /label="71-F"
     rep_origin      complement(2514..3023)
                     /label="M13 ori"
     primer          complement(2546..2564)
                     /label="80-R"
     primer          3000..3017
                     /label="560-F"
     primer          3024..3041
                     /label="pLit-ori-seq-F (338-F)"
     primer          complement(3114..3133)
                     /label="pLit-ori-R (Nr. 337)"
     rep_origin      3134..3722
                     /label="pUC ori"
     mutation        3170
                     /label="Mutation G->A"
     primer          3679..3698
                     /label="pLit-GG-F (Nr. 31-F)"
     primer          3738..3755
                     /label="pLit-ori-F (Nr. 336)"
     primer          3784..3802
                     /label="pLit-ori ROP-F (Nr. 423)"
     promoter        3824..4012
                     /label="rpoN"
     5'UTR           3824..4030
                     /label="rpoN-RBS"
     primer          complement(4003..4030)
                     /label="476-R"
     gene            4031..4248
                     /label="cro"
     primer          complement(4033..4051)
                     /label="490-R"
     gRNA            4053..4072
     PAM             4073..4075
     mutation        4106..4108
                     /label="26N -> 26Y"
     3'UTR           4136..4280
                     /label="TT B0015"
     primer          4158..4178
                     /label="489-F"
     primer          4184..4204
                     /label="566-F"
     primer          4204..4227
                     /label="508-F"
     misc_feature    4229..4248
                     /feature_type="6x frameshift stop"
                     /label="6x frameshift stop"
     primer          4249..4266
                     /label="120-F"
     terminator      4249..4281
                     /label="BBa_B1002"
     mutation        4274
                     /label="Deletion T"
     primer          4282..4302
                     /label="475-F"
ORIGIN
        1 gttaactacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
       61 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      301 gatccttgag agttttcgcc ccgaagaacg ttTCccaatg atgagcactt ttaaagttct
      361 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
      421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
      721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
      781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
      841 tggagccggt gagcgtgggt cccgcggtat cattgcagca ctggggccag atggtaagcc
      901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
      961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1021 ctcataTTTA CGGCTAGCTC AGTCCTAGGT ATAGTGCTAG CgaagacAAA GAtGAGAAAt
     1081 actagGTGAA AAAATTATTA TTCGCAATTC CTTTAGTTGT TCCTTTCTAT TCTCACTCCG
     1141 CTGAAACTGT TGAAAGTTGT TTAGCAAAAT CCCATACAGA AAATTCATTT ACTAACGTCT
     1201 GGAAAGACGA CAAAACTTTA GATCGTTACG CTAACTATGA GGGCTGTCTG TGGAATGCTA
     1261 CAGGCGTTGT AGTTTGTACT GGTGACGAAA CTCAGTGTTA CGGTACATGG GTTCCTATTG
     1321 GGCTTGCTAT CCCTGAAAAT GAGGGTGGTG GCTCTGAGGG TGGCGGTTCT GAGGGTGGCG
     1381 GTTCTGAGGG TGGCGGTACT AAACCTCCTG AGTACGGTGA TACACCTATT CCGGGCTATA
     1441 CTTATATCAA CCCTCTCGAC GGCACTTATC CGCCTGGTAC TGAGCAAAAC CCCGCTAATC
     1501 CTAATCCTTC TCTTGAGGAG TCTCAGCCTC TTAATACTTT CATGTTTCAG AATAATAGGT
     1561 TCCGAAATAG GCAGGGGGCA TTAACTGTTT ATACGGGCAC TGTTACTCAA GGCACTGACC
     1621 CCGTTAAAAC TTATTACCAG TACACTCCTG TATCATCAAA AGCCATGTAT GACGCTTACT
     1681 GGAACGGTAA ATTCAGAGAC TGCGCTTTCC ATTCTGGCTT TAATGAGGAT TTATTTGTTT
     1741 GTGAATATCA AGGCCAATCG TCTGACCTGC CTCAACCTCC TGTCAATGCT GGCGGCGGCT
     1801 CTGGTGGTGG TTCTGGTGGC GGCTCTGAGG GTGGTGGCTC TGAGGGTGGC GGTTCTGAGG
     1861 GTGGCGGCTC TGAGGGAGGC GGTTCCGGTG GTGGCTCTGG TTCCGGTGAT TTTGATTATG
     1921 AAAAGATGGC AAACGCTAAT AAGGGGGCTA TGACCGAAAA TGCCGATGAA AACGCGCTAC
     1981 AGTCTGACGC TAAAGGCAAA CTTGATTCTG TCGCTACTGA TTACGGTGCT GCTATCGATG
     2041 GTTTCATTGG TGACGTTTCC GGCCTTGCTA ATGGTAATGG TGCTACTGGT GATTTTGCTG
     2101 GCTCTAATTC CCAAATGGCT CAAGTCGGTG ACGGTGATAA TTCACCTTTA ATGAATAATT
     2161 TCCGTCAATA TTTACCTTCC CTCCCTCAAT CGGTTGAATG TCGCCCTTTT GTCTTTGGCG
     2221 CTGGTAAACC ATATGAATTT TCTATTGATT GTGACAAAAT AAACTTATTC CGTGGTGTCT
     2281 TTGCGTTTCT TTTATATGTT GCCACCTTTA TGTATGTATT TTCTACGTTT GCTAACATAC
     2341 TGCGTAATAA GGAGTCTTAA GAAGACCCAG GCATCAAATA AAACGAAAGG CTCAGTCGAA
     2401 AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCTACT AGAGTCACAC
     2461 TGGCTCACCT TCGGGTGGGC CTTTCTGCGT TTATAtatac tttagattga tttaccccgg
     2521 ttgataatca gaaaagcccc aaaaacagga agattgtata agcaaatatt taaattgtaa
     2581 acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2641 aataggccga aatcggcaaa atcccttata aatcaaaaga atagcccgag atagggttga
     2701 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2761 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc aaatcaagtt
     2821 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2881 gagcttgacg gggaaagcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     2941 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     3001 taatgcgccg ctacagggcg cgtaaaagga tctaggtgaa gatccttttt gataatctca
     3061 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3121 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttA caaacaaaaa
     3181 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3241 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3301 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3361 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3421 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3481 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3541 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3601 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3661 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3721 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3781 tgtaatgtga gttagctcac tcattaGAAT TCGCTGAGAG ACCCGTGCTG ATCACTGACC
     3841 ACAACGTGCG TGAAACACTG GCGGTTTGTG AACGCGCTTA TATCGTCAGT CAGGGGCATT
     3901 TGATCGCCCA CGGCACGCCT ACAGAAATCT TACAAGACGA ACACGTTAAG CGTGTATACC
     3961 TTGGGGAAGA CTTCAGACTC TGATAGGGTA GAAGTTTGCG ACGTTTTAGC AGGAGAGTAC
     4021 GATTCTGAAC ATGGAACAAC GCATAACCCT GAAAGATTAT GCAATGCGCT TTGGGCAAAC
     4081 CAAGACAGCT AAAGATCTCG GCGTATATCA AAGCGCGATC AACAAGGCCA TTCATGCAGG
     4141 CCGAAAGATT TTTTTAACTA TAAACGCTGA TGGAAGCGTT TATGCGGAAG AGGTAAAGCC
     4201 CTTCCCGAGT AACAAAAAAA CAACAGCAta gtaagtaatg actaatagcg caaaaaaccc
     4261 cgcttcggcg gggtttttcg cGTCTCCtgg cCTGCAGtaa agcccgcttc ggcgggcttt
     4321 ttttt
//
