LOCUS pBbE5a-Opto-Cre-VVD 5720 bp ds-DNA circular 09-OCT-2019 DEFINITION . FEATURES Location/Qualifiers misc_feature 2249..2377 /label="dbl term" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 terminator 3199..3304 /label="T0" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 99..174 /label="lacUV5 promoter" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 887..1345 /label="VVD" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 rep_origin complement(2511..3193) /label="colE1" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac RBS 194..213 /label="RBS" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 1346..1369 /label="GS Linker" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 379..837 /label="VVD" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 343..378 /label="GS Linker" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(3334..4189) /label="Amp" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 214..342 /label="nCre" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 872..886 /label="ATG-DE" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 CDS complement(4368..5450) /label="lacI" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 1370..2224 /label="cCre" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 RBS 844..871 /label="RBS" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 838..843 /label="Two Stop Codons" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 ORIGIN 1 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg 61 tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgtttaggca ccccaggctt 121 tacactttat gcttccggct cgtataatgt gtggaattgt gagcggataa caatttcaga 181 attcaaaaga tcttttaaga aggagatata catATGGCCA CCTCTGATGA AGTCAGGAAG 241 AACCTGATGG ACATGTTCAG GGACAGGCAG GCCTTCTCTG AACACACCTG GAAGATGCTC 301 CTGTCTGTGT GCAGATCCTG GGCTGCCTGG TGCAAGCTGA ACGGGTCTGG CTCCGGATCa 361 agtggtggca gcggtaccca cactctctac gccccaggcg ggtacgatat tatgggctgg 421 ctgatccaga tcatgaacag gcccaatccc caggtcgagc tgggacccgt ggatacttca 481 tgtgcactga tactgtgcga cctgaagcag aaggatacac ctatagttta cgcttcagaa 541 gcctttctgt acatgacagg gtattctaac gccgaggtgc tggggaggaa ctgtaggttc 601 ctccagagtc ccgatggtat ggtgaaacct aagagtactc gcaaatatgt ggatagcaat 661 actattaaca ccatgaggaa agccatcgac agaaacgcag aagttcaggt ggaagtggtg 721 aactttaaga agaacggcca gcggttcgtg aactttctca caatgattcc agtgcgggac 781 gaaaccgggg agtaccggta cagcatgggt tttcagtgcg aaaccgaacc cggataataa 841 tagttaactt taaagaggag aaaggtaccg catgactagc aaaagacaca ctctctacgc 901 cccaggcggg tacgatatta tgggctggct gatccagatc atgaacaggc ccaatcccca 961 ggtcgagctg ggacccgtgg atacttcatg tgcactgata ctgtgcgacc tgaagcagaa 1021 ggatacacct atagtttacg cttcagaagc ctttctgtac atgacagggt attctaacgc 1081 cgaggtgctg gggaggaact gtaggttcct ccagagtccc gatggtatgg tgaaacctaa 1141 gagtactcgc aaatatgtgg atagcaatac tattaacacc atgaggaaag ccatcgacag 1201 aaacgcagaa gttcaggtgg aagtggtgaa ctttaagaag aacggccagc ggttcgtgaa 1261 ctttctcaca atgattccag tgcgggacga aaccggggag taccggtaca gcatgggttt 1321 tcagtgcgaa accgaacccg gataaggtac cgGAGGTTCA GGAGGTTCAA ACAGGAAATG 1381 GTTCCCTGCT GAACCTGAGG ATGTGAGGGA CTACCTCCTG TACCTGCAAG CCAGAGGCCT 1441 GGCTGTGAAG ACCATCCAAC AGCACCTGGG CCAGCTCAAC ATGCTGCACA GGAGATCTGG 1501 CCTGCCTCGC CCTTCTGACT CCAATGCTGT GTCCCTGGTG ATGAGGAGAA TCAGAAAGGA 1561 GAATGTGGAT GCTGGGGAGA GAGCCAAGCA GGCCCTGGCC TTTGAACGCA CTGACTTTGA 1621 CCAAGTCAGA TCCCTGATGG AGAACTCTGA CAGATGCCAG GACATCAGGA ACCTGGCCTT 1681 CCTGGGCATT GCCTACAACA CCCTGCTGCG CATTGCCGAA ATTGCCAGAA TCAGAGTGAA 1741 GGACATCTCC CGCACCGATG GTGGGAGAAT GCTGATCCAC ATTGGCAGGA CCAAGACCCT 1801 GGTGTCCACA GCTGGTGTGG AGAAGGCCCT GTCCCTGGGG GTTACCAAGC TGGTGGAGAG 1861 ATGGATCTCT GTGTCTGGTG TGGCTGATGA CCCCAACAAC TACCTGTTCT GCCGGGTCAG 1921 AAAGAATGGT GTGGCTGCCC CTTCTGCCAC CTCCCAACTG TCCACCCGGG CCCTGGAAGG 1981 GATCTTTGAG GCCACCCACC GCCTGATCTA TGGTGCCAAG GATGACTCTG GGCAGAGATA 2041 CCTGGCCTGG TCTGGCCACT CTGCCAGAGT GGGTGCTGCC AGGGACATGG CCAGGGCTGG 2101 TGTGTCCATC CCTGAAATCA TGCAGGCTGG TGGCTGGACC AATGTGAACA TTGTGATGAA 2161 CTACATCAGA AACCTGGACT CTGAGACTGG GGCCATGGTG AGGCTGCTCG AGGATGGGGA 2221 CTAAggatcc aaactcgagt aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg 2281 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2341 actggctcac cttcgggtgg gcctttctgc gtttatacct agggcgttcg gctgcggcga 2401 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 2461 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 2521 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 2581 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 2641 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 2701 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 2761 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 2821 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 2881 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 2941 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 3001 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 3061 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 3121 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 3181 attttggtca tgactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag 3241 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc 3301 aagcgagctc gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 3361 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 3421 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 3481 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 3541 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 3601 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 3661 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 3721 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 3781 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 3841 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 3901 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 3961 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 4021 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 4081 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 4141 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 4201 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 4261 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 4321 acgtcggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 4381 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 4441 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 4501 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 4561 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 4621 tcggtatcgt cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta 4681 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 4741 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 4801 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 4861 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 4921 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 4981 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 5041 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 5101 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 5161 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 5221 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 5281 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact 5341 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga 5401 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc 5461 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg 5521 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag 5581 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc 5641 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 5701 gagcccgaag tggcgagccc //