LOCUS       pHCL151                 5481 bp ds-DNA     circular     02-MAY-2018
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
COMMENT     lacIq-dsbAss-mScarN
COMMENT     BamHI and HindIII digested pHCL147 was ligated with BglII and
            HindIII digested mScar insert.
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             194..1276
                     /note="LacIq.pep"
                     /locus_tag="LacIq.pep"
                     /label="LacIq.pep"
                     /ApEinfo_label="LacIq.pep"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     DNA             1361..1381
                     /note="lacO"
                     /locus_tag="lacO"
                     /label="lacO"
                     /ApEinfo_label="lacO"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1399..1479
                     /note="LacZ-?.pep"
                     /locus_tag="LacZ-?.pep"
                     /label="LacZ-?.pep"
                     /ApEinfo_label="LacZ-?.pep"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1609..2301
                     /standard_name="mScarlet-Ecoli codon"
                     /locus_tag="mScarlet"
                     /label="mScarlet"
                     /ApEinfo_label="mScarlet"
                     /ApEinfo_fwdcolor="#ff3afc"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     DNA             1443..1459
                     /note="PT7"
                     /locus_tag="PT7"
                     /label="PT7"
                     /ApEinfo_label="PT7"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     DNA             1463..1485
                     /note="lacO"
                     /locus_tag="lacO(1)"
                     /label="lacO(1)"
                     /ApEinfo_label="lacO"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_RNA        1517..1522
                     /note="rbs"
                     /locus_tag="rbs"
                     /label="rbs"
                     /ApEinfo_label="rbs"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1531..1599
                     /note="ss-DsbA"
                     /locus_tag="ss-DsbA"
                     /label="ss-DsbA"
                     /ApEinfo_label="ss-DsbA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     frag            2360..2360
                     /note="1383 to 3311 of pTB314"
                     /locus_tag="1383 to 3311 of pTB314"
                     /label="1383 to 3311 of pTB314"
                     /ApEinfo_label="1383 to 3311 of pTB314"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_RNA        2633..2727
                     /note="tL3 (lambda)"
                     /locus_tag="tL3 (lambda)"
                     /label="tL3 (lambda)"
                     /ApEinfo_label="tL3 (lambda)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_RNA        2783..2793
                     /note="attHKO22"
                     /locus_tag="attHKO22"
                     /label="attHKO22"
                     /ApEinfo_label="attHKO22"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_RNA        join(2907..3016,3018..3303)
                     /note="oriR6K [Split]"
                     /locus_tag="oriR6K [Split]"
                     /label="oriR6K [Split]"
                     /ApEinfo_label="oriR6K [Split]"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(3502..4707)
                     /gene="tetA"
                     /locus_tag="tetA"
                     /label="tetA"
                     /ApEinfo_label="tetA"
                     /ApEinfo_fwdcolor="#ff86de"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            4786..5412
                     /gene="tetR"
                     /locus_tag="tetR"
                     /label="tetR"
                     /ApEinfo_label="tetR"
                     /ApEinfo_fwdcolor="#ff86de"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2388..2409)
                     /locus_tag="pCRIM-Rev"
                     /label="pCRIM-Rev"
                     /ApEinfo_label="pCRIM-Rev"
                     /ApEinfo_fwdcolor="#fff91b"
                     /ApEinfo_revcolor="#ffc408"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1387..1407
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1443..1462
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1359..1381
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1462..1484
                     /locus_tag="LacO(1)"
                     /label="LacO(1)"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1328..1347
                     /locus_tag="plac-seqF"
                     /label="plac-seqF"
                     /ApEinfo_label="plac-seqF"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1663..1683)
                     /locus_tag="E-mScarseqR"
                     /label="E-mScarseqR"
                     /ApEinfo_label="E-mScarseqR"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2359..2359
                     /standard_name="mScarlet-Ecoli codon"
                     /locus_tag="mScarlet(1)"
                     /label="mScarlet(1)"
                     /ApEinfo_label="mScarlet"
                     /ApEinfo_fwdcolor="#ff3afc"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2353..2358
                     /standard_name="LEGPAGL linker"
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#efff3c"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2308..2352
                     /standard_name="LEGPAGL linker"
                     /locus_tag="linker(1)"
                     /label="linker(1)"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#efff3c"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2237..2256
                     /locus_tag="E-mScarseqF"
                     /label="E-mScarseqF"
                     /ApEinfo_label="E-mScarseqF"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 AGATCTGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA
       61 CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTTCAACAA TTCGGGACAC
      121 CATCGAATGG TGCAAAACCT TTCGCGGTAT GGCATGATAG CGCCCGGAAG AGAGTCAATT
      181 CAGGGTGGTG AATGTGAAAC CAGTAACGTT ATACGATGTC GCAGAGTATG CCGGTGTCTC
      241 TTATCAGACC GTTTCCCGCG TGGTGAACCA GGCCAGCCAC GTTTCTGCGA AAACGCGGGA
      301 AAAAGTGGAA GCGGCGATGG CGGAGCTGAA TTACATTCCC AACCGCGTGG CACAACAACT
      361 GGCGGGCAAA CAGTCGTTGC TGATTGGCGT TGCCACCTCC AGTCTGGCCC TGCACGCGCC
      421 GTCGCAAATT GTCGCGGCGA TTAAATCTCG CGCCGATCAA CTGGGTGCCA GCGTGGTGGT
      481 GTCGATGGTA GAACGAAGCG GCGTCGAAGC CTGTAAAGCG GCGGTGCACA ATCTTCTCGC
      541 GCAACGCGTC AGTGGGCTGA TCATTAACTA TCCGCTGGAT GACCAGGATG CCATTGCTGT
      601 GGAAGCTGCC TGCACTAATG TTCCGGCGTT ATTTCTTGAT GTCTCTGACC AGACACCCAT
      661 CAACAGTATT ATTTTCTCCC ATGAAGACGG TACGCGACTG GGCGTGGAGC ATCTGGTCGC
      721 ATTGGGTCAC CAGCAAATCG CGCTGTTAGC GGGCCCATTA AGTTCTGTCT CGGCGCGTCT
      781 GCGTCTGGCT GGCTGGCATA AATATCTCAC TCGCAATCAA ATTCAGCCGA TAGCGGAACG
      841 GGAAGGCGAC TGGAGTGCCA TGTCCGGTTT TCAACAAACC ATGCAAATGC TGAATGAGGG
      901 CATCGTTCCC ACTGCGATGC TGGTTGCCAA CGATCAGATG GCGCTGGGCG CAATGCGCGC
      961 CATTACCGAG TCCGGGCTGC GCGTTGGTGC GGATATCTCG GTAGTGGGAT ACGACGATAC
     1021 CGAAGACAGC TCATGTTATA TCCCGCCGTC AACCACCATC AAACAGGATT TTCGCCTGCT
     1081 GGGGCAAACC AGCGTGGACC GCTTGCTGCA ACTCTCTCAG GGCCAGGCGG TGAAGGGCAA
     1141 TCAGCTGTTG CCCGTCTCAC TGGTGAAAAG AAAAACCACC CTGGCGCCCA ATACGCAAAC
     1201 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT
     1261 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC
     1321 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT
     1381 TTCACACAGG AAACAGCTAT GACCATGATT ACGAATTCCC GGGGATCTCG ATCCCGCGAA
     1441 ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC AATTCCCCTC TAGAAATAAT
     1501 TTTGTTTAAC TTTAAGAAGG AGATATACAT ATGAAAAAGA TTTGGCTGGC GCTGGCTGGT
     1561 TTAGTTTTAG CGTTTAGCGC ATCGGCGGCG CAGTATGAAG GATCTatggt ttctaaaggt
     1621 gaagcagtta tcaaggagtt catgcgcttt aaggttcaca tggaagggag cgttaacgga
     1681 cacgagttcg agatcgaagg ggagggggaa ggccgccctt atgagggtac tcaaactgcc
     1741 aagctgaagg tcacaaaggg gggtcccttg ccgttttctt gggacattct gtcacctcag
     1801 ttcatgtacg ggtcccgcgc gtttattaag catccggctg acatcccaga ttactataag
     1861 cagtcattcc cggaagggtt caaatgggaa cgcgtgatga acttcgaaga tgggggagcc
     1921 gtcactgtta cccaggacac tagcctggag gatggcacgt tgatttataa ggtcaagctg
     1981 cgtggaacaa attttccccc agatgggcca gttatgcaga agaaaacaat ggggtgggaa
     2041 gcgagtacgg agcgcttata ccccgaagat ggcgtcctga aaggggacat caaaatggcc
     2101 ctgcgcctga aggacggggg acgttattta gcggacttca agacaaccta taaagctaag
     2161 aaacccgtgc aaatgcccgg tgcttacaac gtggaccgca agctggatat cacaagccat
     2221 aatgaagatt acaccgtggt agagcagtac gagcgcagtg agggccgcca ctcaaccgga
     2281 ggtatggatg aactgtataa gtctggcggt ggttctccgg ctggtctggg cggtggatcc
     2341 ggtatctcat ccctcgagtA AGCTTATCGA TCTCACGATA ATATCCGGGT AGGCGCAATC
     2401 ACTTTCGTCT CTACTCCGTT ACAAAGCGAG GCTGGGTATT TCCCGGCCTT TCTGTTATCC
     2461 GAAATCCACT GAAAGCACAG CGGCTGGCTG AGGAGATAAA TAATAAACGA GGGGCTGTAT
     2521 GCACAAAGCA TCTTCTGTTG AGTTAAGAAC GAGTATCGAG ATGGCACATA GCCTTGCTCA
     2581 AATTGGAATC AGGTTTGTGC CAATACCAGT AGAAACAGAC GAAGAAGCTA GCTAATGCTC
     2641 TGTCTCAGGT CACTAATACT ATCTAAGTAG TTGATTCATA GTGACTGGAT ATGTTGCGTT
     2701 TTGTCGCATT ATGTAGTCTA TCATTTAACC ACAGATTAGT GTAATGCGAT GATTTTTAAG
     2761 TGATTAATGT TATTTTGTCA TCCTTTAGGT GAATAAGTTG TATATTTAAA ATCTCTTTAA
     2821 TTATCAGTAA ATTAATGTAA GTAGGTCATT ATTAGTCAAA ATAAAATCAT TTGTCGATTT
     2881 CAATTTTGTC CCATGGCTAA TTCCCATGTC AGCCGTTAAG TGTTCCTGTG TCACTCAAAA
     2941 TTGCTTTGAG AGGCTCTAAG GGCTTCTCAG TGCGTTACAT CCCTGGCTTG TTGTCCACAA
     3001 CCGTTAAACC TTAAAACCTT TAAAAGCCTT ATATATTCTT TTTTTTCTTA TAAAACTTAA
     3061 AACCTTAGAG GCTATTTAAG TTGCTGATTT ATATTAATTT TATTGTTCAA ACATGAGAGC
     3121 TTAGTACGTG AAACATGAGA GCTTAGTACG TTAGCCATGA GAGCTTAGTA CGTTAGCCAT
     3181 GAGGGTTTAG TTCGTTAAAC ATGAGAGCTT AGTACGTTAA ACATGAGAGC TTAGTACGTG
     3241 AAACATGAGA GCTTAGTACG TACTATCAAC AGGTTGAACT GCTGATCTTC AGATCCTCTA
     3301 CGCCGGACGC ATCGTGGCCG GATCTTGCGG CCGCAGGCCA ATTTATTGCT ATTTACCGCG
     3361 GCTTTTTATT GAGCTTGAAA GATAAATAAA ATAGATAGGT TTTATTTGAA GCTAAATCTT
     3421 CTTTATCGTA AAAAATGCCC TCTTGGGTTA TCAAGAGGGT CATTATATTT CGCGGAATAA
     3481 CATCATTTGG TGACGAAATA ACTAAGCACT TGTCTCCTGT TTACTCCCCT GAGCTTGAGG
     3541 GGTTAACATG AAGGTCATCG ATAGCAGGAT AATAATACAG TAAAACGCTA AACCAATAAT
     3601 CCAAATCCAG CCATCCCAAA TTGGTAGTGA ATGATTATAA ATAACAGCAA ACAGTAATGG
     3661 GCCAATAACA CCGGTTGCAT TGGTAAGGCT CACCAATAAT CCCTGTAAAG CACCTTGCTG
     3721 ATGACTCTTT GTTTGGATAG ACATCACTCC CTGTAATGCA GGTAAAGCGA TCCCACCACC
     3781 AGCCAATAAA ATTAAAACAG GGAAAACTAA CCAACCTTCA GATATAAACG CTAAAAAGGC
     3841 AAATGCACTA CTATCTGCAA TAAATCCGAG CAGTACTGCC GTTTTTTCGC CCCATTTAGT
     3901 GGCTATTCTT CCTGCCACAA AGGCTTGGAA TACTGAGTGT AAAAGACCAA GACCCGCTAA
     3961 TGAAAAGCCA ACCATCATGC TATTCCATCC AAAACGATTT TCGGTAAATA GCACCCACAC
     4021 CGTTGCGGGA ATTTGGCCTA TCAATTGCGC TGAAAAATAA ATAATCAACA AAATGGGCAT
     4081 CGTTTTAAAT AAAGTGATGT ATACCGAGTT CGATTGCGTC TCAACCCCTA CTTCGGTATC
     4141 TGTATTATCA CGTGTATTTT TGGTTTCACG GAACCAAAAC ATAACCACAA GGAAAGTGAC
     4201 AATATTTAGC AACGCAGCGA TAAAAAAGGG ACTATGCGGT GAAATCTCTC CTGCAAAACC
     4261 ACCAATAATA GGCCCCGCTA TTAAACCAAG CCCAAAACTT GCCCCTAACC AACCGAACCA
     4321 CTTCACGCGT TGAGAAGCTG AGGTGGTATC GGCAATGACC GATGCCGCGA CAGCCCCAGT
     4381 AGCTCCTGTG ATCCCTGAAA GCAAACGGCC TAAATACAGC ATCCAAAGCG CACTTGAAAA
     4441 AGCCAGCAAT AAGTAATCCA GCGATGCGCC TATTAATGAC AACAACAGCA CTGGGCGCCG
     4501 ACCAAATCGG TCAGACATTT TTCCAAGCCA AGGAGCAAAG ATAACCTGCA TTAACGCATA
     4561 AAGTGCAAGC AATACGCCAA AGTGGTTAGC GATATCTTCC GAAGCAATAA ATTCACGTAA
     4621 TAACGTTGGC AAGACTGGCA TGATAAGGCC AATCCCCATG GCATCGAGTA ACGTAATTAC
     4681 CAATGCGATC TTTGTCGAAC TATTCATTTC ACTTTTCTCT ATCACTGATA GGGAGTGGTA
     4741 AAATAACTCT ATCAATGATA GAGTGTCAAC AAAAATTAGG AATTAATGAT GTCCAGATTA
     4801 GATAAAAGTA AAGTGATTAA CAGCGCATTA GAGCTGCTTA ATGAGGTCGG AATCGAAGGT
     4861 TTAACAACCC GTAAACTCGC CCAGAAGCTA GGTGTAGAGC AGCCTACATT GTATTGGCAT
     4921 GTAAAAAATA AGCGGGCTTT GCTCGACGCC TTAGCCATTG AGATGTTAGA TAGGCACCAT
     4981 ACTCACTTTT GCCCTTTAGA AGGGGAAAGC TGGCAAGATT TTTTACGTAA TAACGCTAAA
     5041 AGTTTTAGAT GTGCTTTACT AAGTCATCGC GATGGAGCAA AAGTACATTT AGGTACACGG
     5101 CCTACAGAAA AACAGTATGA AACTCTCGAA AATCAATTAG CCTTTTTATG CCAACAAGGT
     5161 TTTTCACTAG AGAATGCATT ATATGCACTC AGCGCTGTGG GGCATTTTAC TTTAGGTTGC
     5221 GTATTGGAAG ATCAAGAGCA TCAAGTCGCT AAAGAAGAAA GGGAAACACC TACTACTGAT
     5281 AGTATGCCGC CATTATTACG ACAAGCTATC GAATTATTTG ATCACCAAGG TGCAGAGCCA
     5341 GCCTTCTTAT TCGGCCTTGA ATTGATCATC TGCGGATTAG AAAAACAACT TAAATGTGAA
     5401 AGTGGGTCTT AAAAGCAGCA TAACCTTTTT CCGTGATGGT AACTTCACGG TAACCAAGAT
     5461 GTCGAGTTAA CCACCCTTTA G   
//
