LOCUS       Exported                6502 bp ds-DNA     circular SYN 03-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 3, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
COMMENT     LOCUS pCMV-mYFP-C2 4735 bp DNA circular 10-MAY-2006 SOURCE ORGANISM 
            COMMENT http://www.informaxinc.com/ COMMENT This file is created by 
            Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT
            VNTDATE|401540396| COMMENT VNTDBDATE|410099829| COMMENT LSOWNER| 
            COMMENT VNTNAME|pCMV-mYFP-C2| COMMENT VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV Promoter
                     /note="Human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    613..1329
                     /label=mYFP
     misc_feature    1345..3129
                     /label=Nop56
                     /note="Nop56"
     polyA_signal    3321..3326
                     /label=SV40 polyA
                     /note="SV40 mRNA polyadenylation signal"
     polyA_signal    3350..3355
                     /label=SV40 polyA
                     /note="SV40 mRNA polyadenylation signal"
     rep_origin      3418..3873
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 single-strand DNA origin"
     promoter        4047..4118
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4119..4190
                     /label=SV40 promoter
     rep_origin      4214..4349
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     exon            4398..5192
                     /label=Kan/Neo resistance
                     /note="Kanamycin/neomycin resistance gene"
     polyA_signal    5428..5433
                     /label=HSV TK polyA
                     /note="Herpes simplex virus (HSV) thymidine kinase (TK) 
                     polyadenylation signal"
     polyA_signal    5441..5446
                     /label=HSV TK polyA
                     /note="Herpes simplex virus (HSV) thymidine kinase (TK) 
                     polyadenylation signal"
     rep_origin      5777..6420
                     /direction=RIGHT
                     /label=pUC ori
                     /note="pUC plasmid replication origin"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccttcggc tacggcctga agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt actcagatct cgagatggtg ctgttgcacg tgctgtttga gcacgcggtc
     1381 ggctacgcgc tgctggcgct gaaggaagtg gaggagatca gtctgctgca gccgcaggtg
     1441 gaggagtctg tgctcaacct gggcaaattc cacagcatcg ttcgtctggt ggccttttgt
     1501 ccctttgcct catcccaggt tgccttggaa aatgccaacg ccgtgtctga aggggttgtt
     1561 catgaggacc tccgcctgct cttggagacc cacctgccgt ccaaaaagaa gaaagtactc
     1621 ttgggagttg gggatcccaa gattggtgcc gcaatacagg aggagttagg gtacaactgc
     1681 cagactggag gagtcatagc tgagatcctg cgaggagttc gtctgcactt ccacaatctg
     1741 gtgaagggtc tgaccgatct gtcagcttgt aaagcacagc tggggctggg acacagctat
     1801 tcccgtgcca aagttaagtt taatgtgaac cgggtggaca atatgatcat ccagtccatt
     1861 agcctcctgg accagctgga taaggacatc aataccttct ctatgcgtgt cagggagtgg
     1921 tacgggtatc actttccgga gctggtgaag atcatcaacg acaatgccac atactgccgt
     1981 cttgcccagt ttattggaaa ccgaagggaa ctgaatgagg acaagctgga gaagctggag
     2041 gagctgacaa tggatggggc caaggctaag gctattctgg atgcctcacg gtcctccatg
     2101 ggcatggaca tatctgccat tgacttgata aacatcgaga gcttctccag tcgtgtggtg
     2161 tctttatctg aataccgcca gagcctacac acttacctgc gctccaagat gagccaagta
     2221 gcccccagcc tgtcagccct aattggggaa gcggtaggtg cacgtctcat cgcacatgct
     2281 ggcagcctca ccaacctggc caagtatcca gcatccacag tgcagatcct tggggctgaa
     2341 aaggccctgt tcagagccct gaagacaagg ggtaacactc caaaatatgg actcattttc
     2401 cactccacct tcattggccg agcagctgcc aagaacaaag gccgcatctc ccgatacctg
     2461 gcaaacaaat gcagtattgc ctcacgaatc gattgcttct ctgaggtgcc cacgagtgta
     2521 ttcggggaga agcttcgaga acaagttgaa gagcgactgt ccttctatga gactggagag
     2581 ataccacgaa agaatctgga tgtcatgaag gaagcaatgg ttcaggcaga ggaagcggct
     2641 gctgagatta ctaggaagct ggagaaacag gagaagaaac gcttaaagaa ggaaaagaaa
     2701 cggctggctg cacttgccct cgcgtcttca gaaaacagca gtagtactcc agaggagtgt
     2761 gaggagatga gtgaaaaacc caaaaagaag aaaaagcaaa agccccagga ggttcctcag
     2821 gagaatggaa tggaagaccc atctatctct ttctccaaac ccaagaaaaa gaaatctttt
     2881 tccaaggagg agttgatgag tagcgatctt gaagagaccg ctggcagcac cagtattccc
     2941 aagaggaaga agtctacacc caaggaggaa acagttaatg accctgagga ggcaggccac
     3001 agaagtggct ccaagaaaaa gaggaaattc tccaaagagg agccggtcag cagtgggcct
     3061 gaagaggcgg ttggcaagag cagctccaag aagaagaaaa agttccataa agcatcccag
     3121 gaagattagg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata
     3181 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     3241 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     3301 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3361 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa
     3421 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     3481 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     3541 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     3601 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     3661 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     3721 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     3781 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     3841 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     3901 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3961 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     4021 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4081 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4141 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4201 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4261 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4321 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     4381 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     4441 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     4501 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     4561 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     4621 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     4681 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     4741 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     4801 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     4861 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     4921 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     4981 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     5041 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     5101 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     5161 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     5221 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     5281 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     5341 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     5401 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     5461 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     5521 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     5581 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     5641 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5701 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5761 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5821 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5881 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5941 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6001 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6061 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6121 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6181 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6241 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6301 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6361 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6421 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6481 taaccgtatt accgccatgc at
//
