LOCUS       Exported                5688 bp ds-DNA     circular SYN 03-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 3, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
COMMENT     LOCUS pCMV-mYFP-C1 4731 bp DNA circular 19-AUG-2006 SOURCE ORGANISM 
            COMMENT http://www.informaxinc.com/ COMMENT This file is created by 
            Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT
            VNTDATE|401545066| COMMENT VNTDBDATE|419176208| COMMENT LSOWNER| 
            COMMENT VNTNAME|pCMV-mYFP-C1| COMMENT VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV Promoter
                     /note="Human cytomegalovirus (CMV) immediate promoter"
     misc_feature    613..1329
                     /label=mYFP
     primer_bind     1266..1287
                     /label=EGFPC
     misc_feature    1381..2343
                     /label=Fibrillarin
                     /note="Fibrillarin"
     primer_bind     complement(2428..2447)
                     /label=EGFPC Rev
     polyA_signal    2507..2512
                     /label=SV40 polyA
                     /note="SV40 Early mRNA polyadenylation signal"
     polyA_signal    2536..2541
                     /label=SV40 polyA
                     /note="SV40 Early mRNA polyadenylation signal"
     rep_origin      2604..3059
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 single-stranded DNA origin"
     rep_origin      3400..3535
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="SV40 Origin of Replication"
     exon            3584..4378
                     /label=Kan/neo resistance
                     /note="Kanamycin/neomycin resistance gene"
     polyA_signal    4614..4619
                     /label=HSV TK polyA
                     /note="Herpes simplex virus thymidine kinase (TK) 
                     polyadenylation signal"
     polyA_signal    4627..4632
                     /label=HSV TK polyA
                     /note="Herpes simplex virus (HSV) thymidine kinase (TK) 
                     polyadenylation signal"
     rep_origin      4963..5606
                     /direction=RIGHT
                     /label=pUC ori
                     /note="pUC plasmid replication origin"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccttcggc tacggcctga agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1381 atgaagccag gattcagtcc ccgtgggggt ggctttggcg gccgaggggg ctttggtgac
     1441 cgtggtggtc gtggaggccg agggggcttt ggcgggggcc gaggtcgagg cggaggcttt
     1501 agaggtcgtg gacgaggagg aggtggaggc ggcggcggcg gtggaggagg aggaagaggt
     1561 ggtggaggct tccattctgg tggcaaccgg ggtcgtggtc ggggaggaaa aagaggaaac
     1621 cagtcgggga agaatgtgat ggtggagccg catcggcatg agggtgtctt catttgtcga
     1681 ggaaaggaag atgcactggt caccaagaac ctggtccctg gggaatcagt ttatggagag
     1741 aagagagtct cgatttcgga aggagatgac aaaattgagt accgagcctg gaaccccttc
     1801 cgctccaagc tagcagcagc aatcctgggt ggtgtggacc agatccacat caaaccgggg
     1861 gctaaggttc tctacctcgg ggctgcctcg ggcaccacgg tctcccatgt ctctgacatc
     1921 gttggtccgg atggtctagt ctatgcagtc gagttctccc accgctctgg ccgtgacctc
     1981 attaacttgg ccaagaagag gaccaacatc attcctgtga tcgaggatgc tcgacaccca
     2041 cacaaatacc gcatgctcat cgcaatggtg gatgtgatct ttgctgatgt ggcccagcca
     2101 gaccagaccc ggattgtggc cctgaatgcc cacaccttcc tgcgtaatgg aggacacttt
     2161 gtgatttcca ttaaggccaa ctgcattgac tccacagcct cagccgaggc cgtgtttgcc
     2221 tccgaagtga aaaagatgca acaggagaac atgaagccgc aggagcagtt gacccttgag
     2281 ccatatgaaa gagaccatgc cgtggtcgtg ggagtgtaca ggccaccccc caaggtgaag
     2341 aactgaggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     2401 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     2461 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     2521 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     2581 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     2641 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     2701 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     2761 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     2821 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     2881 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     2941 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     3001 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     3061 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3121 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3181 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     3241 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3301 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3361 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3421 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3481 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3541 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     3601 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     3661 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     3721 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     3781 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     3841 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     3901 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3961 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     4021 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     4081 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     4141 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     4201 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     4261 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     4321 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     4381 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     4441 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     4501 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     4561 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     4621 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     4681 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     4741 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     4801 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     4861 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4921 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4981 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5041 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5101 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     5161 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5221 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5281 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5341 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5401 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5461 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5521 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5581 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5641 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
