LOCUS       Exported                6168 bp ds-DNA     circular SYN 03-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 3, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
COMMENT     LOCUS pCMV-mYFP-C1 4731 bp DNA circular 19-AUG-2006 SOURCE ORGANISM 
            COMMENT http://www.informaxinc.com/ COMMENT This file is created by 
            Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT
            VNTDATE|401545066| COMMENT VNTDBDATE|419176208| COMMENT LSOWNER| 
            COMMENT VNTNAME|pCMV-mYFP-C1| COMMENT VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV Promoter
                     /note="Human cytomegalovirus (CMV) immediate promoter"
     misc_feature    613..1329
                     /label=mYFP
     primer_bind     1266..1287
                     /label=EGFPC
     misc_feature    1330..2854
                     /label=MCS
                     /note="Multi Cloning Site"
     gene            1391..2839
                     /label=Sp100A(K297R)
     misc_feature    1391..2839
                     /label=Untitled
     primer_bind     complement(2908..2927)
                     /label=EGFPC Rev
     polyA_signal    2987..2992
                     /label=SV40 polyA
                     /note="SV40 Early mRNA polyadenylation signal"
     polyA_signal    3016..3021
                     /label=SV40 polyA
                     /note="SV40 Early mRNA polyadenylation signal"
     rep_origin      3084..3539
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 single-stranded DNA origin"
     rep_origin      3880..4015
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="SV40 Origin of Replication"
     exon            4064..4858
                     /label=Kan/neo resistance
                     /note="Kanamycin/neomycin resistance gene"
     polyA_signal    5094..5099
                     /label=HSV TK polyA
                     /note="Herpes simplex virus thymidine kinase (TK) 
                     polyadenylation signal"
     polyA_signal    5107..5112
                     /label=HSV TK polyA
                     /note="Herpes simplex virus (HSV) thymidine kinase (TK) 
                     polyadenylation signal"
     rep_origin      5443..6086
                     /direction=RIGHT
                     /label=pUC ori
                     /note="pUC plasmid replication origin"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccttcggc tacggcctga agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1381 gcgggcccgg gatctatggc aggtggaggc ggcgacctga gcaccaggag gctgaatgaa
     1441 tgtatttcac cagtagcaaa tgagatgaac catcttcctg cacacagcca cgatttgcaa
     1501 aggatgttca cggaagacca gggtgtagat gacaggttgc tctatgacat tgtattcaag
     1561 cacttcaaaa gaaataaggt ggagatttca aatgcaataa aaaagacatt tccattcctc
     1621 gagggcctcc gtgatcgtga tctcatcaca aataaaatgt ttgaagattc tcaagattct
     1681 tgtagaaacc tggtccctgt acagagagtg gtgtacaatg ttcttagtga actggagaag
     1741 acatttaacc tgccagttct ggaagcactg ttcagcgatg tcaacatgca ggaatacccc
     1801 gatttaattc acatttataa aggctttgaa aatgtaatcc atgacaaatt gcctctccaa
     1861 gaaagtgaag aagaagagag ggaggagagg tctggcctcc aactaagtct tgaacaagga
     1921 actggtgaaa actcttttcg aagcctgact tggccacctt cgggttcccc atctcatgct
     1981 ggtacaaccc cacctgaaaa tggactctca gagcacccct gtgaaacaga acagataaat
     2041 gcaaagagaa aagatacaac cagtgacaaa gatgattcgc taggaagcca acaaacaaat
     2101 gaacaatgtg ctcaaaaggc tgagccaaca gagtcctgcg aacaaattgc tgtccaagtg
     2161 aataatgggg atgctggaag ggagatgccc tgcccgttgc cctgtgatga agaaagccca
     2221 gaggcagagc tacacaacca tggaatccaa attaattcct gttctgtgcg actggtggat
     2281 ataagaaagg aaaagccatt ttctaattca aaagttgagt gccaagccca agcaagaact
     2341 catcataacc aggcatctga cataatagtc atcagcagtg aggactctga aggatccact
     2401 gacgttgatg agcccttaga agtcttcatc tcagcaccga gaagcgaacc agtcattaat
     2461 aatgacaacc ctttagaatc aaatgatgaa aaggagggcc aagaagccac ttgctcacga
     2521 ccccagattg taccagagcc catggatttc agaaaattat ctacattcag agaaagtttt
     2581 aagaaaagag tgataggaca agaccacgac ttttcagaat ccagtgagga ggaggcgccc
     2641 gcagaagcct caagcggggc actgagaagc aagcatggtg agaaggctcc tatgacttct
     2701 agaagtacat ctacttggag aatacccagc aggaagagac gtttcagcag tagtgacttt
     2761 tcagacctga gtaatggaga agagcttcag gaaacctgca gctcatccct aagaagaggg
     2821 tcaggtaaag aagattaggc tagataactg atcataatca gccataccac atttgtagag
     2881 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     2941 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3001 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3061 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     3121 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     3181 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     3241 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     3301 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     3361 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     3421 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     3481 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     3541 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3601 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3661 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     3721 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3781 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3841 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3901 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3961 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     4021 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     4081 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     4141 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     4201 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     4261 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     4321 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     4381 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     4441 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     4501 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     4561 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     4621 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     4681 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     4741 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     4801 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     4861 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     4921 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     4981 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     5041 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     5101 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     5161 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     5221 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     5281 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     5341 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5401 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5461 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5521 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5581 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     5641 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5701 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5761 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5821 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5881 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5941 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6001 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6061 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6121 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
