LOCUS       Exported                6777 bp ds-DNA     circular SYN 03-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 3, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
COMMENT     LOCUS pCMV-mYFP-C3 4727 bp DNA circular 19-AUG-2006 SOURCE ORGANISM 
            COMMENT http://www.informaxinc.com/ COMMENT This file is created by 
            Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT
            VNTDATE|401540113| COMMENT VNTDBDATE|419180945| COMMENT LSOWNER| 
            COMMENT VNTNAME|pCMV-mYFP-C3| COMMENT VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..589)
                     /label=CMV
                     /note="Human Cytomagalo Virus early promoter"
     misc_feature    613..1329
                     /label=mYFP
     misc_feature    1328..3463
                     /label=MCS
                     /note="Multi cloning site"
     misc_feature    complement(1348..1353)
                     /label=HindIII
                     /note="HindIII"
     gene            1354..3420
                     /label=Sp100B
     misc_feature    1354..3420
                     /label=Untitled
     misc_feature    3421..3426
                     /label=KpnI
                     /note="KpnI"
     polyA_signal    3596..3601
                     /label=SV40 polya
                     /note="SV40 early mRNA poly adenylation signal"
     polyA_signal    3625..3630
                     /label=SV40 polya
                     /note="SV40 early mRNA poly adenylation signal"
     rep_origin      3693..4148
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 single-strand DNA origin"
     promoter        4322..4393
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4394..4465
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4489..4624
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="SV40 origin of Replication"
     exon            4673..5467
                     /label=Kan/neo reistance
                     /note="Kanamycin/neomycin resistance gene"
     polyA_signal    5703..5708
                     /label=HSV TK polyA
                     /note="Herpes simplex virus thymidine kinase 
                     polyadenylation signal"
     polyA_signal    5716..5721
                     /label=HSV TK polyA
                     /note="Herpes simplex virus thymidine kinase 
                     polyadenylation signal"
     rep_origin      6052..6695
                     /direction=RIGHT
                     /label=pUC ori
                     /note="pUC plasmid replication origin"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccttcggc tacggcctga agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt actcagatct cgagctcaag cttatggcag gtggaggcgg cgacctgagc
     1381 accaggaggc tgaatgaatg tatttcacca gtagcaaatg agatgaacca tcttcctgca
     1441 cacagccacg atttgcaaag gatgttcacg gaagaccagg gtgtagatga caggttgctc
     1501 tatgacattg tattcaagca cttcaaaaga aataaggtgg agatttcaaa tgcaataaaa
     1561 aagacatttc cattcctcga gggcctccgt gatcgtgatc tcatcacaaa taaaatgttt
     1621 gaagattctc aagattcttg tagaaacctg gtccctgtac agagagtggt gtacaatgtt
     1681 cttagtgaac tggagaagac atttaacctg ccagttctgg aagcactgtt cagcgatgtc
     1741 aacatgcagg aataccccga tttaattcac atttataaag gctttgaaaa tgtaatccat
     1801 gacaaattgc ctctccaaga aagtgaagaa gaagagaggg aggagaggtc tggcctccaa
     1861 ctaagtcttg aacaaggaac tggtgaaaac tcttttcgaa gcctgacttg gccaccttcg
     1921 ggttccccat ctcatgctgg tacaacccca cctgaaaatg gactctcaga gcacccctgt
     1981 gaaacagaac agataaatgc aaagagaaaa gatacaacca gtgacaaaga tgattcgcta
     2041 ggaagccaac aaacaaatga acaatgtgct caaaaggctg agccaacaga gtcctgcgaa
     2101 caaattgctg tccaagtgaa taatggggat gctggaaggg agatgccctg cccgttgccc
     2161 tgtgatgaag aaagcccaga ggcagagcta cacaaccatg gaatccaaat taattcctgt
     2221 tctgtgcgac tggtggatat aaaaaaggaa aagccatttt ctaattcaaa agttgagtgc
     2281 caagcccaag caagaactca tcataaccag gcatctgaca taatagtcat cagcagtgag
     2341 gactctgaag gatccactga cgttgatgag cccttagaag tcttcatctc agcaccgaga
     2401 agtgagcctg tgatcaataa tgacaaccct ttagaatcaa atgatgaaaa ggagggccaa
     2461 gaagccactt gctcacgacc ccagattgta ccagagccca tggatttcag aaaattatct
     2521 acattcagag aaagttttaa gaaaagagtg ataggacaag accacgactt ttcagaatcc
     2581 agtgaggagg aggcgcccgc agaagcctca agcggggcac tgagaagcaa gcatggtgag
     2641 aaggctccta tgacttctag aagtacatct acttggagaa tacccagcag gaagagacgt
     2701 ttcagcagta gtgacttttc agacctgagt aatggagaag agcttcagga aacctgcagc
     2761 tcatccctaa gaagagggtc aggatcacag ccacaagaac ctgaaaataa gaagtgctcc
     2821 tgtgtcatgt gttttccaaa aggtgtgcca agaagccaag aagcaaggac tgaaagtagt
     2881 caagcatctg acatgatgga taccatggat gttgaaaaca attctacttt ggaaaaacac
     2941 agtgggaaaa gaagaaaaaa gagaaggcat agatctaaag taaatggtct ccaaagaggg
     3001 agaaagaaag acagacctag aaaacattta actctgaata acaaagtcca aaagaaaaga
     3061 tggcaacaaa gaggaagaaa agccaacact agacctttga aaagaagaag aaaaagaggt
     3121 ccaagaattc ccaaagatga aaatattaat tttaaacaat ctgaacttcc tgtgacctgt
     3181 ggtgaggtga agggcactct atataaggag cgattcaaac aaggaacctc aaagaagtgt
     3241 atacagagtg aggataaaaa gtggttcact cccagggaat ttgaaattga aggagaccgc
     3301 ggagcatcca agaactggaa gctaagtata cgctgcggtg gatataccct gaaagtcctg
     3361 atggagaaca aatttctgcc agaaccacca agcacaagaa aaaaggtgat gatcaagtga
     3421 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca
     3481 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     3541 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     3601 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3661 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
     3721 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3781 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3841 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3901 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3961 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4021 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4081 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4141 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4201 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4261 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     4321 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4381 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4441 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4501 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4561 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4621 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     4681 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4741 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4801 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     4861 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4921 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4981 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5041 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5101 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5161 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     5221 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5281 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5341 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5401 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5461 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     5521 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5581 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     5641 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     5701 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     5761 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     5821 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     5881 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     5941 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6001 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6061 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6181 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6481 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6721 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
