LOCUS       Exported                7044 bp ds-DNA     circular SYN 03-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 3, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
COMMENT     LOCUS pCMV-mYFP-C2 4735 bp DNA circular 10-MAY-2006 SOURCE ORGANISM 
            COMMENT http://www.informaxinc.com/ COMMENT This file is created by 
            Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT
            VNTDATE|401540396| COMMENT VNTDBDATE|410099829| COMMENT LSOWNER| 
            COMMENT VNTNAME|pCMV-mYFP-C2| COMMENT VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV Promoter
                     /note="Human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    613..1329
                     /label=mYFP
     misc_feature    1334..3730
                     /label=MCS
                     /note="Multiple cloning site"
     misc_feature    1339..1343
                     /label=Blunt end XhoI site at 5' MCS in YFP
                     /note="Blunt end XhoI site at 5' MCS in YFP vector"
     misc_feature    1344..3671
                     /label=Untitled
     CDS             1344..3671
                     /label=ICP0 cDNA (2-775)
     primer_bind     1344..1357
                     /label=ICP0-EcoR1-Dir
     misc_feature    1344..1347
                     /label=Blunt end NcoI site at 5' end of ICP0
                     /note="Blunt end NcoI site at 5' end of ICP0"
     misc_feature    1369..1377
                     /label=TRR
     primer_bind     2676..2683
                     /label=miR-H2
     polyA_signal    3863..3868
                     /label=SV40 polyA
                     /note="SV40 mRNA polyadenylation signal"
     polyA_signal    3892..3897
                     /label=SV40 polyA
                     /note="SV40 mRNA polyadenylation signal"
     rep_origin      3960..4415
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 single-strand DNA origin"
     promoter        4589..4660
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4661..4732
                     /label=SV40 promoter
     rep_origin      4756..4891
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     exon            4940..5734
                     /label=Kan/Neo resistance
                     /note="Kanamycin/neomycin resistance gene"
     polyA_signal    5970..5975
                     /label=HSV TK polyA
                     /note="Herpes simplex virus (HSV) thymidine kinase (TK) 
                     polyadenylation signal"
     polyA_signal    5983..5988
                     /label=HSV TK polyA
                     /note="Herpes simplex virus (HSV) thymidine kinase (TK) 
                     polyadenylation signal"
     rep_origin      6319..6962
                     /direction=RIGHT
                     /label=pUC ori
                     /note="pUC plasmid replication origin"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccttcggc tacggcctga agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt actcagatct cgacatggag ccccgccccg gagcgagtac ccgccggcct
     1381 gagggccgcc cccagcgcga gcccgccccg gatgtctggg tgtttccctg cgaccgagac
     1441 ctgccggaca gcagcgactc tgaggcggag accgaagtgg gggggcgggg ggacgccgac
     1501 caccatgacg acgactccgc ctccgaggcg gacagcacgg acacggaact gttcgagacg
     1561 gggctgctgg ggccgcaggg cgtggatggg ggggcggtct cgggggggag ccccccccgc
     1621 gaggaagacc ccggcagttg cgggggcgcc ccccctcgag aggacggggg gagcgacgag
     1681 ggcgacgtgt gcgccgtgtg cacggatgag atcgcgcccc acctgcgctg cgacaccttc
     1741 ccgtgcatgc accgcttctg catcccgtgc atgaaaacct ggatgcaatt gcgcaacacc
     1801 tgcccgctgt gcaacgccaa gctggtgtac ctgatagtgg gcgtgacgcc cagcgggtcg
     1861 ttcagcacca tcccgatcgt gaacgacccc cagacccgca tggaggccga ggaggccgtc
     1921 agggcgggca cggccgtgga ctttatctgg acgggcaatc agcggttcgc cccgcggtac
     1981 ctgaccctgg gggggcacac ggtgagggcc ctgtcgccca cccacccgga gcccaccacg
     2041 gacgaggatg acgacgacct ggacgacgca gactacgtac cgcccgcccc ccgccggacg
     2101 ccccgcgccc ccccacgcag aggcgccgcc gcgccccccg tgacgggcgg ggcgtctcac
     2161 gcagcccccc agccggccgc ggctcggaca gcgcccccct cggcgcccat cgggccacac
     2221 ggcagcagta acaccaacac caccaccaac agcagcggcg gcggcggctc ccgccagtcg
     2281 cgagccgcgg cgccgcgggg ggcgtctggc ccctccgggg gggttggggt tggggttggg
     2341 gttgttgaag cggaggcggg gcggccgagg ggccggacgg gcccccttgt caacagaccc
     2401 gccccccttg caaacaacag agaccccata gtgatcagcg actccccccc ggcctctccc
     2461 cacaggcccc ccgcggcgcc catgccaggc tccgcccccc gccccgggcc ccccgcgtcc
     2521 gcggccgcgt cgggacccgc gcgcccccgc gcggccgtgg ccccgtgcgt gcgagcgccg
     2581 cctccggggc ccggcccccg cgccccggcc cccggggcgg agccggccgc ccgccccgcg
     2641 gacgcgcgcc gtgtgcccca gtcgcactcg tccctggctc aggccgcgaa ccaagaacag
     2701 agtctgtgcc gggcgcgtgc gacggtggcg cgcggctcgg gggggccggg cgtggagggt
     2761 gggcacgggc cctcccgcgg cgccgccccc tccggcgccg ccccgctccc ctccgccgcc
     2821 tctgtcgagc aggaggcggc ggtgcgtccg aggaagaggc gcgggtcggg ccaggaaaac
     2881 ccctcccccc agtccacgcg tccccccctc gcgccggcag gggccaagag ggcggcgacg
     2941 caccccccct ccgactcagg gccggggggg cgcggccagg gtgggcccgg gacccccctg
     3001 acgtcctcgg cggcctccgc ctcttcctcc tctgcctctt cctcctcggc cccgaccccc
     3061 gcgggggccg cctcttccgc cgccggggcc gcgtcctcct ccgcttccgc ctcctcgggc
     3121 ggggccgtcg gtgccctggg agggagacaa gaggaaacct ccctcggccc ccgcgctgct
     3181 tctgggccgc gggggccgag gaagtgtgcc cggaagacgc gccacgcgga gacttccggg
     3241 gccgtccccg cgggcggcct cacgcgctac ctgcccatct cgggggtctc tagcgtggtc
     3301 gccctgtcgc cttacgtgaa caagactatc acgggggact gcctgcccat cctggacatg
     3361 gagacgggga acatcggggc gtacgtggtc ctggtggacc agacgggaaa catggcgacc
     3421 cggctgcggg ccgcggtccc cggctggagc cgccgcaccc tgctccccga gaccgcgggt
     3481 aaccacgtga tgccccccga gtacccgacg gcccccgcgt cggagtggaa cagcctctgg
     3541 atgacccccg tggggaacat gctgttcgac cagggcaccc tagtgggcgc cctggacttc
     3601 cgcagcctgc ggtctcggca cccgtggtcc ggggagcagg gggcgtcgac ccgggacgag
     3661 ggaaaacaat agaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga
     3721 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     3781 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     3841 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3901 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
     3961 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     4021 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     4081 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     4141 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     4201 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     4261 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     4321 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     4381 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     4441 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4501 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     4561 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4621 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4681 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4741 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4801 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     4861 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     4921 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     4981 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5041 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5101 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5161 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5221 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5281 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5341 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5401 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5461 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     5521 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     5581 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     5641 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     5701 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     5761 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     5821 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     5881 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     5941 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     6001 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     6061 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     6121 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     6181 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6241 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6301 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6361 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6421 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6481 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6661 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6721 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6781 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6841 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6901 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6961 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7021 gataaccgta ttaccgccat gcat
//
