LOCUS       802__FD__pFlag__        5787 bp ds-DNA     circular     07-OCT-2019
DEFINITION  
ACCESSION   124 pFLAG-AID wrong
SOURCE      Homo sapiens (unknown)
  ORGANISM  Homo sapiens Homo sapiens Eukaryota; Metazoa; Chordata; Craniata;
            Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini;
            Hominidae; Homo
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             604..643
                     /note="CLC name: FLAG-wrong-AID"
                     /locus_tag="CLC name: FLAG-wrong-AID"
                     /label="CLC name: FLAG-wrong-AID"
                     /ApEinfo_label="CLC name: FLAG-wrong-AID"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          join(644..648,2431..2435)
                     /organism="Cloning vector pEGFP-C3"
                     /mol_type="other DNA"
                     /db_xref="taxon:47841"
                     /locus_tag="source:Cloning vector pEGFP-C3"
                     /label="source:Cloning vector pEGFP-C3"
                     /ApEinfo_label="source:Cloning vector pEGFP-C3"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3683..4477
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB08061.1"
                     /db_xref="GI:1373320"
                     /note="Oriinal location: 2623..3417"
                     /note="CLC name: Neomycin"
                     /locus_tag="neomycin phosphotransferase"
                     /label="neomycin phosphotransferase"
                     /ApEinfo_label="neomycin phosphotransferase"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(644..648,2431..2435)
                     /gene="egfp"
                     /codon_start=1
                     /transl_table=11
                     /product="Enhanced Green Fluorescent Protein"
                     /protein_id="AAB08060.1"
                     /db_xref="GI:1373319"
                     /locus_tag="Enhanced Green Fluorescent Protein"
                     /label="Enhanced Green Fluorescent Protein"
                     /ApEinfo_label="Enhanced Green Fluorescent Protein"
                     /ApEinfo_fwdcolor="#5aff67"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    604..627
                     /locus_tag="flag"
                     /label="flag"
                     /ApEinfo_label="flag"
                     /ApEinfo_fwdcolor="#ff7517"
                     /ApEinfo_revcolor="#ff7517"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2436..2462
                     /note="CLC name: FLAG-wrong-AID"
                     /locus_tag="CLC name: FLAG-wrong-AID(1)"
                     /label="CLC name: FLAG-wrong-AID(1)"
                     /ApEinfo_label="CLC name: FLAG-wrong-AID"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            join(644..648,2431..2435)
                     /gene="egfp"
                     /locus_tag="egfp"
                     /label="egfp"
                     /ApEinfo_label="egfp"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    649..2430
                     /locus_tag="RMB47 CDS"
                     /label="RMB47 CDS"
                     /ApEinfo_label="RMB47 CDS"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    705..705
                     /locus_tag="silent mut"
                     /label="silent mut"
                     /ApEinfo_label="silent mut"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG
       61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
      121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
      181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
      241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
      301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
      361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG
      421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG
      481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT
      541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGTCG
      601 ACCATGGACT ACAAGGACGA TGATGACAAG CTTAACTCAG ATCTCGAGAT GACCGCAGAG
      661 GATTCCACCG CAGCCATGAG CAGTGACTCG GCCGCCGGGT CCTCgGCCAA GGTGCCCGAG
      721 GGCGTGGCGG GCGCGCCCAA CGAGGCAGCA CTGCTGGCGC TGATGGAGCG CACGGGCTAC
      781 AGCATGGTGC AAGAGAACGG GCAGCGCAAG TACGGCGGCC CACCGCCCGG CTGGGAGGGC
      841 CCGCACCCGC AGCGTGGCTG CGAGGTCTTC GTGGGCAAGA TCCCGCGCGA CGTGTACGAG
      901 GACGAGCTGG TGCCCGTGTT CGAGGCCGTG GGCCGCATCT ACGAGCTGCG CCTCATGATG
      961 GACTTTGACG GCAAGAACCG CGGCTACGCC TTCGTCATGT ACTGCCACAA GCACGAGGCC
     1021 AAGCGCGCAG TGCGTGAGCT CAACAACTAC GAGATCCGCC CGGGCCGCCT GCTCGGCGTG
     1081 TGCTGCAGCG TGGACAACTG CCGCCTCTTC ATCGGCGGGA TCCCCAAGAT GAAGAAGCGC
     1141 GAGGAAATCC TGGAGGAGAT TGCCAAGGTC ACCGAGGGCG TGCTGGACGT GATCGTCTAC
     1201 GCCAGCGCGG CCGACAAGAT GAAGAACCGC GGCTTCGCCT TCGTGGAGTA CGAGAGCCAC
     1261 CGCGCGGCTG CCATGGCTCG CCGCAAGCTC ATGCCTGGCC GCATCCAGCT GTGGGGCCAC
     1321 CAGATCGCCG TGGACTGGGC CGAACCTGAG ATCGACGTGG ACGAGGACGT GATGGAGACC
     1381 GTGAAGATCC TCTACGTGCG CAACCTCATG ATCGAGACCA CCGAGGACAC CATCAAGAAG
     1441 AGCTTCGGCC AGTTCAACCC CGGCTGCGTG GAGCGCGTCA AGAAGATCCG CGACTACGCC
     1501 TTCGTGCACT TCACCAGCCG CGAGGATGCC GTGCATGCCA TGAACAACCT CAACGGCACT
     1561 GAGCTGGAGG GCTCGTGCCT GGAGGTCACG CTGGCCAAGC CCGTGGACAA GGAGCAGTAC
     1621 TCGCGCTACC AGAAGGCAGC CAGGGGCGGC GGCGCGGCTG AGGCAGCGCA GCAGCCCAGC
     1681 TACGTGTACT CCTGCGACCC CTACACACTG GCCTACTACG GCTACCCCTA CAACGCGCTC
     1741 ATTGGGCCCA ACAGGGACTA CTTTGTGAAA GCAGGCAGCA TAAGAGGCCG AGGGCGAGGT
     1801 GCAGCTGGCA ACAGAGCCCC AGGGCCTAGG GGTTCCTACC TCGGGGGATA TTCTGCTGGT
     1861 CGTGGTATAT ATAGCCGATA TCATGAAGGG AAAGGAAAGC AGCAAGAAAA AGGATATGAA
     1921 CTGGTGCCGA ATTTGGAAAT CCCTACCGTC AACCCAGTTG CCATTAAACC TGGTACAGTA
     1981 GCCATCCCTG CCATTGGGGC TCAGTATTCC ATGTTTCCAG CAGCTCCAGC CCCTAAAATG
     2041 ATTGAAGATG GCAAAATCCA CACAGTGGAG CACATGATCA GCCCCATTGC TGTGCAGCCA
     2101 GACCCAGCCA GTGCTGCTGC CGCCGCAGCC GCGGCCGCAG CCGCCGCAGC CGCTGTCATT
     2161 CCCACTGTGT CGACGCCACC ACCTTTCCAG GGCCGCCCAA TAACTCCAGT ATACACGGTG
     2221 GCTCCAAACG TTCAGAGAAT TCCTACTGCC GGGATCTACG GGGCCAGTTA CGTGCCATTT
     2281 GCTGCTCCAG CTACAGCCAC GATCGCCACA CTACAGAAGA ACGCGGCAGC CGCGGCCGCC
     2341 ATGTATGGAG GATACGCAGG CTACATACCT CAGGCCTTCC CTGCTGCTGC CATTCAGGTC
     2401 CCCATCCCCG ACGTCTACCA GACATACTGA GGTACCGCGG GCCCGGGATC CACCGGATCT
     2461 AGATAACTGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC
     2521 CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG
     2581 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA
     2641 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAACGC
     2701 GTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
     2761 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
     2821 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
     2881 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
     2941 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
     3001 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
     3061 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
     3121 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCA GGTGGCACTT TTCGGGGAAA
     3181 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
     3241 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTC CTGAGGCGGA
     3301 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA
     3361 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
     3421 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC
     3481 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC
     3541 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA
     3601 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA
     3661 GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC
     3721 CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA
     3781 TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT
     3841 GTCCGGTGCC CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC
     3901 GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT
     3961 ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT
     4021 ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT
     4081 CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT
     4141 CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG
     4201 GCTCAAGGCG AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT
     4261 GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG
     4321 TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG
     4381 CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG
     4441 CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG
     4501 ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT
     4561 GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG
     4621 GATCTCATGC TGGAGTTCTT CGCCCACCCT AGGGGGAGGC TAACTGAAAC ACGGAAGGAG
     4681 ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGT
     4741 GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC
     4801 CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC
     4861 CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA
     4921 GCCTCAGGTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG
     4981 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG
     5041 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT
     5101 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG
     5161 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA
     5221 CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA
     5281 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG
     5341 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC
     5401 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA
     5461 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG
     5521 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC
     5581 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG
     5641 TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG
     5701 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT
     5761 GTGGATAACC GTATTACCGC CATGCAT   
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