LOCUS Exported 9059 bp ds-DNA circular SYN 16-SEP-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pTF101.3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9059) AUTHORS . TITLE Direct Submission JOURNAL Exported Wednesday, Oct 9, 2019 from SnapGene Viewer 4.3.11 https://www.snapgene.com COMMENT ORIGDB|GenBank FEATURES Location/Qualifiers source 1..9059 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 1187..3318 /label=pVS1 ori rep_origin 3922..4836 /label=pBR322 ori CDS complement(5082..6091) /codon_start=3 /label=aadA /note="/vntifkey=4" /translation="PSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQARY AVGRCLMLWSSNDVTQQGSRPKTKLNIMGEAVIAEVSTQLSEVVGVIERHLEPTLLAVH LYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIV VHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAA EELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAA DWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" misc_signal 6396..6421 /label=LB /label=L25 /note="/vntifkey=87" terminator 6661..7220 /label=Tvsp /note="/vntifkey=43" CDS complement(7224..7771) /codon_start=1 /label=Bar /note="/vntifkey=4" /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF WQLDFSLPVPPRPVLPVTE" promoter complement(7793..8538) /label=2 X 35S /label=2\X\35S /note="/vntifkey=29" misc_signal 8891..8916 /label=RB /note="/vntifkey=87" ORIGIN 1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag 61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg 121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac 181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc 241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc 301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc 361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg 421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc 481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg 541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc 601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg 661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg 721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa 781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc 841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg 901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg 961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg 1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc 1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg 1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc 1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc 1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg 1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac 1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc 1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt 1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg 1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg 1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga 1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct 1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa 1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta 1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac 1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag 1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag 2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg 2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg 2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg 2221 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc 2281 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg 2341 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc 2401 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag 2461 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt 2521 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag 2581 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg 2641 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg 2701 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg 2761 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa 2821 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac 2881 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag 2941 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag 3001 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat 3061 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc 3121 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc 3181 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag 3241 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa 3301 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa 3361 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac 3421 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac 3481 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa 3541 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc 3601 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg 3661 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta 3721 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc 3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata 4921 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc 4981 tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg 5041 ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg 5101 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag 5161 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg 5221 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt 5281 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag 5341 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca 5401 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt 5461 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca 5521 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac 5581 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct 5641 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca 5701 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc 5761 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg 5821 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt 5881 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa 5941 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca 6001 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg 6061 gtcaaggttc tggaccagtt gcgtgacggc agttacgcta cttgcattac agcttacgaa 6121 ccgaacgagg cttatgtcca ctgggttcgt gcccgaattg atcacaggca gcaacgctct 6181 gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag 6241 cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac 6301 ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt 6361 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa 6421 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa 6481 cgccgaattg ctctagcatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 6541 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 6601 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 6661 aattcgcttc aagacgtgct caaatcacta tttccacacc cctatatttc tattgcactc 6721 ccttttaact gttttttatt acaaaaatgc cctggaaaat gcactccctt tttgtgtttg 6781 tttttttgtg aaacgatgtt gtcaggtaat ttatttgtca gtctactatg gtggcccatt 6841 atattaatag caactgtcgg tccaatagac gacgtcgatt ttctgcattt gtttaaccac 6901 gtggatttta tgacatttta tattagttaa tttgtaaaac ctacccaatt aaagacctca 6961 tatgttctaa agactaatac ttaatgataa caattttctt ttagtgaaga aagggataat 7021 tagtaaatat ggaacaaggg cagaagattt attaaagccg cggtaagaga caacaagtag 7081 gtacgtggag tgtcttaggt gacttaccca cataacataa agtgacatta acaaacatag 7141 ctaatgctcc tatttgaata gtgcatatca gcatacctta ttacatatag ataggagcaa 7201 actctagcta gattgttgag cagatctcgg tgacgggcag gaccggacgg ggcggtaccg 7261 gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg 7321 ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt 7381 cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca 7441 gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg gagacgtaca 7501 cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt ccaggggccc gcgtaggcga 7561 tgccggcgac ctcgccgtcc acctcggcga cgagccaggg atagcgctcc cgcagacgga 7621 cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt acggaagttg accgtgcttg 7681 tctcgatgta gtggttgacg atggtgcaga ccgccggcat gtccgcctcg gtggcacggc 7741 ggatgtcggc cgggcgtcgt tctgggctca tggtagatcc ccaattctcg aggtcctctc 7801 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc 7861 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg 7921 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc 7981 ggtagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggagcc 8041 accttccttt tccactatct tcacaataaa gtgacagata gctgggcaat ggaatccgag 8101 gaggtttccg gatattaccc tttgttgaaa agtctcaatt gccctttggt cttctgagac 8161 tgtatctttg atatttttgg agtagacaag tgtgtcgtgc tccaccatgt tatcacatca 8221 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg 8281 tgggggtcca tctttgggac cactgtcggc agaggcatct tcaacgatgg cctttccttt 8341 atcgcaatga tggcatttgt aggagccacc ttccttttcc actatcttca caataaagtg 8401 acagatagct gggcaatgga atccgaggag gtttccggat attacccttt gttgaaaagt 8461 ctcaattgcc ctttggtctt ctgagactgt atctttgata tttttggagt agacaagtgt 8521 gtcgtgctcc accatgttga cctgcaggca tgcaagcttg catgcctgca ggtcgactct 8581 agaggatccc cgggtaccga gctcgaattc gtaatcatgt catagctgtt tcctgtgtga 8641 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 8701 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 8761 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 8821 ggtttgcgta ttggagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac 8881 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga 8941 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat 9001 gccaaccaca gggttcccct cgggatcaaa gtactttaaa gtactttaaa gtactttaa //