LOCUS       pED1a71:_357-AGGA       5455 bp ds-DNA     circular     25-JUN-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     -35_signal      1069..1074
                     /label="-35"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        1063..1103
                     /label="proD"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      566..997
                     /label="rrnB1 transcriptional terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     -35_signal      973..978
                     /label="A (0.81)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     modified_base   1657..1668
                     /label="USER Junction"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     sig_peptide     2266..2325
                     /label="GGGGS4 flexible linker"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3307..3323
                     /label="USER junction"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     modified_base   4290..4305
                     /label="USER Junction"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1182..3306
                     /label="xluxAB"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        complement(4196..4289)
                     /label="KanR Promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     modified_base   1151..1166
                     /label="USER Junction"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(3380..4195)
                     /label="Kan"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     RBS             1167..1181
                     /label="SD8"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     modified_base   3367..3379
                     /label="USER junction"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     -10_signal      1092..1097
                     /label="-10"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(4306..5256)
                     /label="RepA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     modified_base   551..565
                     /label="USER junction"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4978..4980
                     /label=""
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     modified_base   1671..1673
                     /label="Quadruplet Codon Mods"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     prim_transcript 1104..1104
                     /label="+1"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     terminator      complement(3331..3366)
                     /label="P14/tonB bidirectional terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     modified_base   998..1013
                     /label="USER Linker"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(join(5260..5455,1..550))
                     /label="pSC101"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct
       61 gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg
      121 tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc
      181 tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct
      241 ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca
      301 ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag aaaagcccgt
      361 cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct gactttttgc
      421 tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta tcccgtgaca
      481 ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca ggcttacccg
      541 tcttactgtc aagaggacat ccggtggctg ttttggcgga tgagagaaga ttttcagcct
      601 gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag
      661 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
      721 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa
      781 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
      841 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
      901 ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga
      961 cggatggcct ttttgcgttt ctacaaactc gctgaggatt ctagagcaca gctaacacca
     1021 cgtcgtccct atctgctgcc ctaggtctat gagtggttgc tggataactt tacgggcatg
     1081 cataaggctc gtataatata ttcagggaga ccacaacggt ttccctctac aaataatttt
     1141 gtttaacttt atatctgtta ttttttaagg aggaaaaaaa aatgaaattt ggaaactttt
     1201 tgcttacata ccaacctccc caattttccc aaacagaggt aatgaaacgt ttggttaaat
     1261 taggtcgcat ctctgaggag tgtggttttg ataccgtatg gttactggag catcatttca
     1321 cggagtttgg tttgcttggt aacccttatg tcgctgctgc atatttactt ggcgcgacta
     1381 aaaaattgaa tgtaggaact gccgctattg ttcttcccac agcccatcca gtacgccaac
     1441 ttgaagatgt gaatttattg gatcaaatgt caaaaggacg atttcggttt ggtatttgcc
     1501 gagggcttta caacaaggac tttcgcgtat tcggaacaga tatgaataac agtcgcgcct
     1561 tagcggaatg ctggtacggg ctgataaaga atggcatgac agagggatat atggaagctg
     1621 ataatgaaca tatcaagttc cataaggtaa aagtaaaccc cgcggcgtat agcagaggtg
     1681 gcgcaccggt ttatgtggtg gctgaatcag cttcgacgac tgagtgggct gctcaatttg
     1741 gcctaccgat gatattaagt tggattataa atactaacga aaagaaagca caacttgagc
     1801 tttataatga agtggctcaa gaatatgggc acgatattca taatatcgac cattgcttat
     1861 catatataac atctgtagat catgactcaa ttaaagcgaa agagatttgc cggaaatttc
     1921 tggggcattg gtatgattct tatgtgaatg ctacgactat ttttgatgat tcagaccaaa
     1981 caagaggtta tgatttcaat aaagggcagt ggcgtgactt tgtattaaaa ggacataaag
     2041 atactaatcg ccgtattgat tacagttacg aaatcaatcc cgtgggaacg ccgcaggaat
     2101 gtattgacat aattcaaaaa gacattgatg ctacaggaat atcaaatatt tgttgtggat
     2161 ttgaagctaa tggaacagta gacgaaatta ttgcttccat gaagctcttc cagtctgatg
     2221 tcatgccatt tcttaaagaa aaacaacgtA GGActattat attatggcgg tggcggtagc
     2281 ggcggtggcg gtagcggcgg tggcggtagc ggcggtggcg gtagcaaatt tggattgttc
     2341 ttccttaact tcatcaattc aacaactgtt caagaacaga gtatagttcg catgcaggaa
     2401 ataacggagt atgttgataa gttgaatttt gaacagattt tagtgtatga aaatcatttt
     2461 tcagataatg gtgttgtcgg cgctcctctg actgtttctg gttttctgct cggtttaaca
     2521 gagaaaatta aaattggttc attaaatcac atcattacaa ctcatcatcc tgtccgcata
     2581 gcggaggaag cttgcttatt ggatcagtta agtgaaggga gatttatttt agggtttagt
     2641 gattgcgaaa aaaaagatga aatgcatttt tttaatcgcc cggttgaata tcaacagcaa
     2701 ctatttgaag agtgttatga aatcattaac gatgctttaa caacaggcta ttgtaatcca
     2761 gataacgatt tttatagctt ccctaaaata tctgtaaatc cccatgctta tacgccaggc
     2821 ggacctcgga aatatgtaac agcaaccagt catcatattg ttgagtgggc ggccaaaaaa
     2881 ggtattcctc tcatctttaa gtgggatgat tctaatgatg ttagatatga atatgctgaa
     2941 agatataaag ccgttgcgga taaatatgac gttgacctat cagagataga ccatcagtta
     3001 atgatattag ttaactataa cgaagatagt aataaagcta aacaagagac gcgtgcattt
     3061 attagtgatt atgttcttga aatgcaccct aatgaaaatt tcgaaaataa acttgaagaa
     3121 ataattgcag aaaacgctgt cggaaattat acggagtgta taactgcggc taagttggca
     3181 attgaaaagt gtggtgcgaa aagtgtattg ctgtcctttg aaccaatgaa tgatttgatg
     3241 agccaaaaaa atgtaatcaa tattgttgat gataatatta agaagtacca cacggaatat
     3301 acctaaactt aattaacggc actcctcagc caagtcaaaa gcctccgacc ggaggctttt
     3361 gactacatgc ccatggcgtt tagaaaaact catcgagcat caaatgaaac tgcaatttat
     3421 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     3481 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     3541 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     3601 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc
     3661 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     3721 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
     3781 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     3841 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     3901 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     3961 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     4021 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     4081 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     4141 tgttggaatt taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcataacac
     4201 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
     4261 cttgtgcaat gtaacatcag agattttgaa ggccaaatag gccgttcaga tccttccgta
     4321 tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc
     4381 attgagatca tgcttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg
     4441 aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttacgta ggtaggaatc
     4501 tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc
     4561 ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg
     4621 gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg
     4681 tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat
     4741 gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag
     4801 ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg
     4861 ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact
     4921 aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatCCG
     4981 aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc
     5041 tttagctaat acaccataag cattttccct actgatgttc atcatctgag cgtattggtt
     5101 ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag
     5161 tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc
     5221 tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt
     5281 ttaatcacta taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg
     5341 agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag
     5401 accctctgta aattccgcta gacctttgtg tgtttttttt gtttatattc aagtg
//