LOCUS       pED14xR-4               3234 bp ds-DNA     circular     05-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     protein_bind    complement(1658..1673)
                     /label="iteron 6"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    complement(1590..1605)
                     /label="iteron 9"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1737..1745)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    complement(1817..1833)
                     /label="iteron 4"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(1350..1876)
                     /label="oriV"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     modified_base   1316..1334
                     /label="USER Junction"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     RBS             complement(1256..1266)
                     /label="~ SD4 RBS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(107..1255)
                     /label="trfA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      1907..1951
                     /label="rrnB1 transcriptional terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    complement(1519..1531)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     modified_base   2435..2447
                     /label="USER junction"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     protein_bind    complement(1681..1696)
                     /label="iteron 5"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2135..2140
                     /label="-10"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    complement(1723..1731)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    complement(1495..1507)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    complement(1613..1628)
                     /label="iteron 8"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2237..2240
                     /label="quad anticodon"
                     /ApEinfo_revcolor=#d1bffc
                     /ApEinfo_fwdcolor=#d1bffc
     protein_bind    complement(1755..1767)
                     /label="IHF BS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     modified_base   1892..1906
                     /label="USER junction"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    complement(1508..1520)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    join(2305..3234,1..2174)
                     /label="BACKBONE"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    complement(1635..1650)
                     /label="iteron 7"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2162..2173
                     /label="USER"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1748..1756)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2203..2280
                     /label="tRNA-Arg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     modified_base   1959..1973
                     /label="USER junction"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1974..2140
                     /label="ProK promoter"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    complement(1483..1494)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      2320..2354
                     /label="rrnC terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      complement(2393..2434)
                     /label="tetA/orfL bidirectional terminator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     protein_bind    complement(1711..1719)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     modified_base   2369..2385
                     /label="USER junction"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        6..90
                     /label="aadA promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    complement(1853..1868)
                     /label="iteron 3"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2235..2242
                     /label="loop"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     protein_bind    complement(1873..1889)
                     /label="iteron 2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     modified_base   complement(1267..1281)
                     /label="USER Junction"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2112..2117
                     /label="-35"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     modified_base   91..106
                     /label="USER junction"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(join(2448..3234,1..2))
                     /label="aadA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(1282..1315)
                     /label="JC23107"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    complement(2141..2161)
                     /label="lacI binding site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
       61 ggatacatat ttgaatgtat ttagaaaaat aggccaaata ggccgtctag cgtttgcaat
      121 gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc
      181 aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga
      241 ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca
      301 tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt
      361 cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct
      421 tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc
      481 ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg
      541 tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg
      601 tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt
      661 cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt
      721 ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca
      781 gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat
      841 cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct
      901 gcttggcctc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg
      961 tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcga
     1021 gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca
     1081 cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg
     1141 tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa
     1201 accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc
     1261 ctccttatat cgtatggggc tgctagcata atacctaggg ctgagctagc cgtaaagtga
     1321 aagaagagaa acctccattt ttggggtgag gccgttcgcg gccgaggggc gcagcccctg
     1381 gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg gcacccccct
     1441 tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata
     1501 ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct
     1561 tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc
     1621 atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg
     1681 cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg
     1741 ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg
     1801 ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc
     1861 aagtgtcaac gtccgcccct catctgtcag taagaggaca tccggtcaaa taaaacgaaa
     1921 ggctcagtcg aaagactggg cctttcgttt tgctgaggag acttagggac ccttgtgctt
     1981 ctcaaatgcc tgaggccagt ttgctcaggc tctccccgtg gaggtaataa ttgacgatat
     2041 gatcagtgca cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
     2101 ctattaaggg attgacgagg gcgtatctgc gcagtaagat aattgtgagc ggataacaat
     2161 tagcagacaa gatgggtccc ttgtagaata agttttcccg atgcatccgt agTtcagctg
     2221 gatagagCac tcggctTCTA aaccgaTcgg tcggaggttc gaatcctccc ggatgcacca
     2281 tattctccgt aaccttcagc aggatccgtc gacctgcaga tccttagcga aagctaagga
     2341 ttttttttaa gcttctgcca ttcatccgac ttaattaacg gcactcctca gcaaatataa
     2401 tgaccctctt gataacccaa gagggcattt tttaatgccc atggcgttta tttgccgact
     2461 accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag
     2521 gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga
     2581 tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg
     2641 ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca
     2701 gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc
     2761 tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt
     2821 tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata
     2881 cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa
     2941 cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg
     3001 ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt
     3061 tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg
     3121 ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg
     3181 atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttcc
//