LOCUS       pED14xS-3               3237 bp ds-DNA     circular     25-JAN-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     protein_bind    complement(1723..1731)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    complement(1711..1719)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2215..2222
                     /label="anticodon loop"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1681..1696)
                     /label="iteron 5"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(107..1255)
                     /label="trfA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     protein_bind    complement(1495..1507)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        6..90
                     /label="aadA promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2217..2220
                     /label="UAGA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        complement(1282..1315)
                     /label="JC23107"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     modified_base   complement(1267..1281)
                     /label="USER Junction"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    complement(1853..1868)
                     /label="iteron 3"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2183..2273
                     /label="SerU"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    complement(1613..1628)
                     /label="iteron 8"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2135..2140
                     /label="-10"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    complement(1590..1605)
                     /label="iteron 9"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1508..1520)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     tRNA            2304..2304
                     /label="amber suppressor tRNA"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     modified_base   1316..1334
                     /label="USER Junction"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     protein_bind    complement(1817..1833)
                     /label="iteron 4"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     modified_base   2438..2450
                     /label="USER junction"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     modified_base   2304..2318
                     /label="USER Junction"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1974..2140
                     /label="ProK promoter"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     modified_base   91..106
                     /label="USER junction"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    complement(1737..1745)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      complement(2396..2437)
                     /label="tetA/orfL bidirectional terminator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      2323..2357
                     /label="rrnC terminator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    complement(1755..1767)
                     /label="IHF BS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    join(2372..3237,1..2173)
                     /label="Backbone"
                     /ApEinfo_revcolor=#f6989d
                     /ApEinfo_fwdcolor=#f6989d
     misc_feature    2162..2173
                     /label="USER"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1658..1673)
                     /label="iteron 6"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(join(2451..3237,1..14))
                     /label="aadA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(1350..1876)
                     /label="oriV"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2112..2117
                     /label="-35"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     modified_base   1892..1906
                     /label="USER junction"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    complement(1873..1889)
                     /label="iteron 2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     modified_base   1959..1973
                     /label="USER junction"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    complement(1519..1531)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     RBS             complement(1256..1266)
                     /label="~ SD4 RBS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    complement(1748..1756)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2236..2236
                     /label="second base of variable loop C->T"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    complement(1635..1650)
                     /label="iteron 7"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(2141..2161)
                     /label="lacI binding site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      1907..1951
                     /label="rrnB1 transcriptional terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    complement(1483..1494)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2183..2371
                     /label="from p239"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     modified_base   2372..2388
                     /label="USER junction"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
       61 ggatacatat ttgaatgtat ttagaaaaat aggccaaata ggccgtctag cgtttgcaat
      121 gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc
      181 aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga
      241 ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca
      301 tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt
      361 cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct
      421 tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc
      481 ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg
      541 tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg
      601 tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt
      661 cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt
      721 ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca
      781 gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat
      841 cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct
      901 gcttggcctc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg
      961 tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcga
     1021 gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca
     1081 cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg
     1141 tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa
     1201 accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc
     1261 ctccttatat cgtatggggc tgctagcata atacctaggg ctgagctagc cgtaaagtga
     1321 aagaagagaa acctccattt ttggggtgag gccgttcgcg gccgaggggc gcagcccctg
     1381 gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg gcacccccct
     1441 tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata
     1501 ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct
     1561 tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc
     1621 atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg
     1681 cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg
     1741 ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg
     1801 ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc
     1861 aagtgtcaac gtccgcccct catctgtcag taagaggaca tccggtcaaa taaaacgaaa
     1921 ggctcagtcg aaagactggg cctttcgttt tgctgaggag acttagggac ccttgtgctt
     1981 ctcaaatgcc tgaggccagt ttgctcaggc tctccccgtg gaggtaataa ttgacgatat
     2041 gatcagtgca cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
     2101 ctattaaggg attgacgagg gcgtatctgc gcagtaagat aattgtgagc ggataacaat
     2161 tAGCAGACAA GATgggtccc ttggagagat gccggagcgg ctgaacggac cggtATTCTA
     2221 ACaccggagt aggggTaact ctaccggggg ttcaaatccc cctctctccg ccactttatc
     2281 aatgacttat ctcccgactt cccaggatcc gtcgacctgc agatccttag cgaaagctaa
     2341 ggattttttt taagcttctg ccattcatcc gacttaatta acggcactcc tcagcaaata
     2401 taatgaccct cttgataacc caagagggca ttttttaatg cccatggcgt ttatttgccg
     2461 actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc
     2521 gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc
     2581 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
     2641 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
     2701 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
     2761 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
     2821 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
     2881 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
     2941 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc
     3001 tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt
     3061 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg
     3121 ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc
     3181 tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttcc
//