LOCUS       pED14xS-11_-_SerU-TAGA- 3232 bp ds-DNA     circular     06-SEP-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     protein_bind    complement(3096..3108)
                     /label="IHF BS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    complement(3078..3086)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     RBS             complement(2597..2607)
                     /label="~ SD4 RBS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    323..326
                     /label="variable loop"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    complement(2954..2969)
                     /label="iteron 8"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    280..282
                     /label="stem"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     modified_base   1432..1447
                     /label="USER junction"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    complement(3194..3209)
                     /label="iteron 3"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    305..308
                     /label="#f58a5e"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     modified_base   460..476
                     /label="USER junction"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    317..322
                     /label="stem"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(1448..2596)
                     /label="trfA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     modified_base   1..15
                     /label="USER junction"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    311..315
                     /label="#faac61"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        1347..1431
                     /label="aadA promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    294..296
                     /label="stem"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    complement(3022..3037)
                     /label="iteron 5"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(539..1341)
                     /label="aadA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    271..277
                     /label="acceptor stem 5'"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    complement(3158..3174)
                     /label="iteron 4"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    303..310
                     /label="#f8d3a9"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    complement(3214..3230)
                     /label="iteron 2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    271..361
                     /label="SerU Magliery"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    244..249
                     /label="-10"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    351..357
                     /label="acceptor stem 3'"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    221..226
                     /label="-35"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    283..293
                     /label="D loop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    complement(3052..3060)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(2836..2848)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    83..249
                     /label="ProK promoter"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    complement(2849..2861)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(2860..2872)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    298..302
                     /label="#c7b0e3"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    complement(2931..2946)
                     /label="iteron 9"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     modified_base   392..396
                     /label="USER Junction"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      16..60
                     /label="rrnB1 transcriptional terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     modified_base   68..82
                     /label="USER junction"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    346..350
                     /label="T loop stem 3'"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     modified_base   526..538
                     /label="USER junction"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     protein_bind    complement(2824..2835)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    complement(3064..3072)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    complement(250..270)
                     /label="lacI binding site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      complement(484..525)
                     /label="tetA/orfL bidirectional terminator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(2623..2656)
                     /label="JC23107"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     terminator      411..445
                     /label="rrnC terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    complement(2999..3014)
                     /label="iteron 6"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(2691..3217)
                     /label="oriV"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     modified_base   2657..2675
                     /label="USER Junction"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    339..345
                     /label="T loop"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    complement(2976..2991)
                     /label="iteron 7"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    327..332
                     /label="stem"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    334..338
                     /label="T loop stem 5'"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    complement(3089..3097)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    359..361
                     /label="#faac61"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    join(397..3232,1..270)
                     /label="BACKBONE"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     modified_base   complement(2608..2622)
                     /label="USER Junction"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 aagaggacat ccggtcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
       61 gctgaggaga cttagggacc cttgtgcttc tcaaatgcct gaggccagtt tgctcaggct
      121 ctccccgtgg aggtaataat tgacgatatg atcagtgcac ggctaactaa gcggcctgct
      181 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
      241 cagtaagata attgtgagcg gataacaatt ggagagatgc cggagcggct gaacggaccg
      301 gtATTCTAAC accggagtag gggcaactct accgggggtt caaatccccc tctctccgcc
      361 aCTTTATCAA TGACTTATCT CCCGACTTCC Caggatccgt cgacctgcag atccttagcg
      421 aaagctaagg atttttttta agcttctgcc attcatccga cttaattaac ggcactcctc
      481 agcaaatata atgaccctct tgataaccca agagggcatt ttttaatgcc catggcgttt
      541 atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat
      601 ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta
      661 tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg
      721 gcgcgatttt gcccaccaag gtagtcggcg gttactgcgc tgtaccaaat gcgggacaac
      781 gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag
      841 gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc
      901 gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca
      961 atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca
     1021 aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg
     1081 gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg
     1141 tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa
     1201 tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc
     1261 agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat
     1321 acttcggcga tcaccgcttc cctcatactc ttcctttttc aatattattg aagcatttat
     1381 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taggccaaat
     1441 aggccgtcta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc
     1501 cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg
     1561 tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt
     1621 gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc
     1681 atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga
     1741 cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca
     1801 ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg
     1861 acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc
     1921 aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca
     1981 acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga
     2041 tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt
     2101 tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg
     2161 tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg
     2221 tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg
     2281 cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca
     2341 aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca
     2401 gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca
     2461 tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg
     2521 tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt
     2581 caaacgtccg attcattcac cctccttata tcgtatgggg ctgctagcat aatacctagg
     2641 gctgagctag ccgtaaagtg aaagaagaga aacctccatt tttggggtga ggccgttcgc
     2701 ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc
     2761 gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt
     2821 taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc
     2881 cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa
     2941 atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg
     3001 cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca
     3061 ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg
     3121 aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg
     3181 cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gt
//