LOCUS       pED14xY-1               3240 bp ds-DNA     circular     13-NOV-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      complement(2399..2440)
                     /label="tetA/orfL bidirectional terminator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2162..2173
                     /label="USER"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1681..1696)
                     /label="iteron 5"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    complement(1723..1731)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    join(2305..3240,1..2174)
                     /label="BACKBONE"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    complement(1508..1520)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1483..1494)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1974..2140
                     /label="ProK promoter"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    complement(1737..1745)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    complement(1635..1650)
                     /label="iteron 7"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1590..1605)
                     /label="iteron 9"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(2141..2161)
                     /label="lacI binding site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2162..2182
                     /label="ED0218"
                     /ApEinfo_revcolor=#00cccc
                     /ApEinfo_fwdcolor=#00cccc
     protein_bind    complement(1817..1833)
                     /label="iteron 4"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    complement(1853..1868)
                     /label="iteron 3"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     modified_base   1316..1334
                     /label="USER Junction"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     modified_base   1892..1906
                     /label="USER junction"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2237..2240
                     /label="quad anticodon"
                     /ApEinfo_revcolor=#d1bffc
                     /ApEinfo_fwdcolor=#d1bffc
     protein_bind    complement(1873..1889)
                     /label="iteron 2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2112..2117
                     /label="-35"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(1282..1315)
                     /label="JC23107"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(join(2454..3240,1..8))
                     /label="aadA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      1907..1951
                     /label="rrnB1 transcriptional terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     RBS             complement(1256..1266)
                     /label="~ SD4 RBS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    complement(1613..1628)
                     /label="iteron 8"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    complement(1711..1719)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1658..1673)
                     /label="iteron 6"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     modified_base   2441..2453
                     /label="USER junction"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     modified_base   1959..1973
                     /label="USER junction"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(1350..1876)
                     /label="oriV"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2203..2288
                     /label="tRNA Tyr"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2307..2329
                     /label="ED0014"
                     /ApEinfo_revcolor=#00cccc
                     /ApEinfo_fwdcolor=#00cccc
     misc_feature    2135..2140
                     /label="-10"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     modified_base   complement(1267..1281)
                     /label="USER Junction"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     modified_base   91..106
                     /label="USER junction"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2235..2242
                     /label="loop"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      2326..2360
                     /label="rrnC terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        6..90
                     /label="aadA promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    complement(1755..1767)
                     /label="IHF BS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     modified_base   2375..2391
                     /label="USER junction"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    complement(1495..1507)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    complement(1748..1756)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1519..1531)
                     /label="DnaA BS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(107..1255)
                     /label="trfA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
       61 ggatacatat ttgaatgtat ttagaaaaat aggccaaata ggccgtctag cgtttgcaat
      121 gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc
      181 aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga
      241 ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca
      301 tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt
      361 cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct
      421 tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc
      481 ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg
      541 tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg
      601 tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt
      661 cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt
      721 ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca
      781 gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat
      841 cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct
      901 gcttggcctc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg
      961 tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcga
     1021 gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca
     1081 cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg
     1141 tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa
     1201 accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc
     1261 ctccttatat cgtatggggc tgctagcata atacctaggg ctgagctagc cgtaaagtga
     1321 aagaagagaa acctccattt ttggggtgag gccgttcgcg gccgaggggc gcagcccctg
     1381 gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg gcacccccct
     1441 tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata
     1501 ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct
     1561 tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc
     1621 atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg
     1681 cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg
     1741 ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg
     1801 ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc
     1861 aagtgtcaac gtccgcccct catctgtcag taagaggaca tccggtcaaa taaaacgaaa
     1921 ggctcagtcg aaagactggg cctttcgttt tgctgaggag acttagggac ccttgtgctt
     1981 ctcaaatgcc tgaggccagt ttgctcaggc tctccccgtg gaggtaataa ttgacgatat
     2041 gatcagtgca cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
     2101 ctattaaggg attgacgagg gcgtatctgc gcagtaagat aattgtgagc ggataacaat
     2161 tagcagacaa gatgggtccc tttagtcggc accatcaagt ccggtggggt tcccgagcgg
     2221 ccaaagggag cagactTCTA aatctgccgt cacagacttc gaaggttcga atccttcccc
     2281 caccaccaat ttcggccacg cgatggagga tccgtcgacc tgcagatcct tagcgaaagc
     2341 taaggatttt ttttaagctt ctgccattca tccgacttaa ttaacggcac tcctcagcaa
     2401 atataatgac cctcttgata acccaagagg gcatttttta atgcccatgg cgtttatttg
     2461 ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg
     2521 cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg
     2581 ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg
     2641 attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca
     2701 tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat
     2761 agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg
     2821 ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg
     2881 aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct
     2941 ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga
     3001 atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc
     3061 gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc
     3121 aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg
     3181 cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc
//