LOCUS       Exported                8663 bp ds-DNA     circular UNA 10-OCT-2019
DEFINITION  Gibson Assembly ligation product.
ACCESSION   urn.local...74-8sossff
VERSION     .
KEYWORDS    pTK-eGFP-Nluc-P2A-neo-TK
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 10, 2019 from SnapGene 5.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     primer_bind     109..124
                     /label=puc19F
                     /note="color: black; sequence: CGTCAGCGGGTGTTGG"
     CDS             complement(join(146..406,6380..6446))
                     /codon_start=1
                     /gene="lacZ"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /note="This feature has 2 segments:
                        1:  146 ..  406 / #993366
                        2: 6380 .. 6446 / #993366"
                     /translation="MTMITPSLHACRSTLEDPRVPS*LEFTGRRFTTS*LGKPWRYPT*
                     SPCSTSPFRQLA**RRGPHRSPFPTVAQPEWRMAPDAVFSPYASVRYFTPHMVHSQYNL
                     L*CRI"
     misc_feature    183..188
                     /label=NdeI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CA^TATG"
                     /note="/annotation_group=CATATG"
                     /note="color: #a6acb3"
     misc_feature    389..406
                     /label=Ligation
                     /label=nonstandard type:  ligation
                     /note="color: #a6acb3"
     misc_feature    407..412
                     /label=SacI, SstI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GAGCT^C"
                     /note="/annotation_group=GAGCTC"
                     /note="color: #a6acb3"
     misc_feature    413..1449
                     /label=5'CpTK
                     /label=TK-eGFP-Nluc-P2A-neo-TK extraction (concatenate
                     /label=TK-eGFP-Nluc-P2A-neo-TK extraction (concatenated
                     sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:1o-8so41ep"
                     /note="color: #a6acb3"
     5'UTR           413..1449
                     /label=5' CpTK
                     /note="/created_by=User"
                     /note="color: #a6acb3"
     misc_feature    1450..2156
                     /label=Actin promoter
                     /label=ACTGCAAATAAGATTGGAAATACG
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=ACTGCAAATAAGATTGGAAATACG"
                     /note="color: #a6acb3"
     5'UTR           1450..2115
                     /label=actin promoter
                     /note="/created_by=Lisa"
                     /note="color: #993366"
     5'UTR           2116..2426
                     /label=actin 5' UTR
                     /note="/created_by=Lisa"
                     /note="color: #a6acb3"
     primer_bind     2211..2230
                     /label=Actin-5UTR-F
                     /note="color: black; sequence: cgatacgtacagtgcgcatt"
     CDS             2427..3146
                     /codon_start=1
                     /label=eGFP
                     /note="color: #00ff00"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     3'UTR           3147..3457
                     /label=3' UTR
                     /note="/created_by=Lisa"
                     /note="color: #a6acb3"
     misc_feature    3458..3707
                     /label=actin homology
                     /note="/created_by=Lisa"
                     /note="color: #a6acb3"
     promoter        3711..4067
                     /label=Enolase promoter
                     /note="/created_by=User"
                     /note="color: #993366"
     misc_feature    4074..4592
                     /label=nLuc
                     /note="/created_by=User"
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    4593..4658
                     /label=P2A
                     /note="/created_by=Lisa"
                     /note="color: #000080"
     misc_feature    4659..5450
                     /label=Neomycin
                     /note="/created_by=User"
                     /note="color: #ccffff; direction: RIGHT"
     3'UTR           5462..6373
                     /label=3' CpTK
                     /note="/created_by=User"
                     /note="color: #a6acb3"
     misc_feature    join(6380..8663,1..406)
                     /label=source synthetic DNA construct
                     /note="/lab_host=Escherichia coli"
                     /note="/mol_type=other DNA"
                     /note="/organism=synthetic DNA construct"
                     /note="color: #a6acb3"
     misc_feature    join(6380..6429,396..406)
                     /label=MCS
                     /note="pUC19 multiple cloning site"
                     /note="This feature has 2 segments:
                        1: 6380 .. 6429 / #a6acb3
                        2:  396 ..  406 / #a6acb3"
     misc_feature    6380..6397
                     /label=Ligation
                     /label=nonstandard type:  ligation
                     /note="color: #a6acb3"
     misc_feature    6385..6390
                     /label=KpnI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGTAC^C"
                     /note="/annotation_group=GGTACC"
                     /note="color: #a6acb3"
     misc_feature    6394..6399
                     /label=BamHI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GATCC"
                     /note="/annotation_group=GGATCC"
                     /note="color: #a6acb3"
     misc_feature    6400..6405
                     /label=XbaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=T^CTAGA"
                     /note="/annotation_group=TCTAGA"
                     /note="color: #a6acb3"
     misc_feature    6406..6411
                     /label=AccI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GT^MKAC"
                     /note="/annotation_group=GTMKAC"
                     /note="color: #a6acb3"
     misc_feature    6406..6411
                     /label=SalI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TCGAC"
                     /note="/annotation_group=GTCGAC"
                     /note="color: #a6acb3"
     misc_feature    6406..6411
                     /label=HincII
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GTY^RAC"
                     /note="/annotation_group=GTYRAC"
                     /note="color: #a6acb3"
     misc_feature    6412..6417
                     /label=PstI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CTGCA^G"
                     /note="/annotation_group=CTGCAG"
                     /note="color: #a6acb3"
     misc_feature    6418..6423
                     /label=SphI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCATG^C"
                     /note="/annotation_group=GCATGC"
                     /note="color: #a6acb3"
     misc_feature    6424..6429
                     /label=HindIII
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=A^AGCTT"
                     /note="/annotation_group=AAGCTT"
                     /note="color: #a6acb3"
     protein_bind    6466..6482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     complement(6474..6493)
                     /label=puc19R
                     /note="color: black; sequence: GTTGTGTGGAATTGTGAGCG"
     promoter        complement(6490..6520)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(6844..7432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffffff"
     CDS             complement(7603..8463)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="color: #333399"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8464..8568)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #993366; direction: LEFT"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctgagc tccatttgtc
      421 ctaacttcat ctatggtctt atttttatca acctgcttaa taaaagagtt caaattccta
      481 tctacaaaaa ttacctggtc ttcatcatct atgtcatctt tcagtaaatg gctttcaaca
      541 attaatgcag tattgtcttt atcatcaata tattctttat caatatcaat cccaatcatt
      601 ccatttttac gttttctacc tttaaatccg ccttcattaa tttcacaatt attgaaaaaa
      661 tcttcgtcca aactactatc attacattca agcttctcag ttaaaatatt gtcaacttta
      721 ttataaaatg taacattttg acaaactatt ggcttttcaa agttatactc ccagtcaggc
      781 ggaatcacct catcatctgg tacaacaaat tcatcagagt ctattgaaag tttatcagaa
      841 tcatctttca atttactttt tttcctcctt ggtcttgttt tctttgactt aactctacgc
      901 tttttcaatt tatctggtgc atcttgattt tgttcatcgc caatcctttt catatagcta
      961 actggattca aaaaattgga attttcctgg taataaactt tatctaattc atctgattct
     1021 tccctcaaat caacaaataa tatttcctct tccttattat acccacctaa taataattca
     1081 agatcctcct cagtaggccc attttcaaga ctacttatca taatttctta tcaaactcaa
     1141 gttaaaaagc attatcacac caaaaacata tattctactc gaaatcccac tcttatataa
     1201 taatatcaat ttacatttca agtatctgaa aatgaaccct tttggcgcct tttaatatct
     1261 atatataggt aaccactttt ctcaagtaat aaatttgagg ctaaattgag attaaataac
     1321 tactcataaa gtaaaaataa attaaattta tttttattaa tcactcaaaa caaaatttta
     1381 aaccatctaa cacaaagaat tcaacaattt acctaataaa tactttaaag caataatatc
     1441 actcatacct cagaatgagt tggttataaa cagtaataat agaaagcgtt agggctataa
     1501 aaatgaccaa ataagtcatt tagcatcata agtatataca ttagatacag aatgagttca
     1561 aaataaataa aaaattagac ttacttacaa attctaagct atagtataaa agaactaaga
     1621 agatttgatg aatttgctat accattttaa atatttgttt atagtttttt gatattaata
     1681 taaggactat taagtgaaaa ggaaactaca catatttccg ccaattcatt gtattgcatc
     1741 gcacacataa atgaaagatt aaaagaaaag aaattctatt atctagtatt aaaataatac
     1801 caataataag caacactttt ttgttatttt ttattataaa gattaatttg ctactttaaa
     1861 ttgaaaaaaa ctttacaggt ttatacaaat tcgtacatca catgtataca atgctacata
     1921 cattacttga taaatattac gacgtgaaac gtattaattt aaacgaatct tagttatgta
     1981 aataaaaaaa ggttagattt tagtaaaaaa aaaagaccaa attttatatt aaatataaat
     2041 cttgcattga atgcatttat gtcaaattgc atgcaccttg ccgcccacca tgcatagacg
     2101 cgcgccaata cgtattaata gacgattaca tgacaaagtt tagagattaa ataaaaatca
     2161 agtataaatt tagaatagac aacatgtaaa aagtgagatg aatcttttta cgatacgtac
     2221 agtgcgcatt aaaaatccaa agtatatttt tattttcctg cgtgcgtata catatcttta
     2281 aatacttgga taaagagata ataagaattg tacggcaagc aaagtagtag tttaatagag
     2341 cagtttttat attttatata tagcttactt acaatataga tttttttttt tcatagattt
     2401 cgggcatatt acacaagtaa caaaagatgg tgagcaaggg cgaggagctg ttcaccgggg
     2461 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgcgcg
     2521 gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc tgcaccaccg
     2581 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     2641 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     2701 gctacgtcca ggagcgcacc atctccttca aggacgacgg cacctacaag acccgcgccg
     2761 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     2821 aggaggacgg caacatcctg gggcacaagc tggagtacaa cttcaacagc cacaacgtct
     2881 atatcacggc cgacaagcag aagaacggca tcaaggcgaa cttcaagatc cgccacaacg
     2941 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     3001 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
     3061 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     3121 tcggcatgga cgagctgtac aagtagatca atattgttct tggagttgtt cttaacagct
     3181 tatttcctga acacagatac gtattgcaac ttaaatagaa atatgaatta cacaagttat
     3241 cacttcttgt agacaatttc ttattacatt attcagatct cagttctatt ctcatttgaa
     3301 agctaaaaaa aaagatgata aattgtctaa tcaatgctaa taaactctaa atttagcaag
     3361 aaaattctat tataattgtt agtcaatgat aactaatatt atggatagaa ggtgggctag
     3421 tttgaatcaa aatagttact aagtaatgtg tagttcgata cgattttttc cttcttttta
     3481 aacgaaagtg tttaaattat gtgcgtgttg ttttcctgag cacagtatta gaaattaatt
     3541 aagttgattt gtcaggtgat gtttattacc aaagttcaag tccgaaaata gagctgccat
     3601 ttaacaataa aaaagagcgg tccaaattta ggcaaaacaa attttttaaa aaaagctcca
     3661 agtccgggaa ttgaacccgg gtcatccgcg tgacaggcgg atatactagg tggggaaact
     3721 aaatatactg aaattcggta gattctatat ctcacgggac agcttttcac acacacttag
     3781 ttctatattg cgtcataact tttgtttttt tttgttgcac tttttttctc ttatattcaa
     3841 gtaagtggtt tagattctct aagggcggga atatgaatta gtggcaattc aaaggattta
     3901 aacgatcagg cgcctgcaca ccaaactcta attcgtacac agcatgcccg aggttataga
     3961 tatagatact gcgaaattat tttcattgtt tcagttaaga aataataaaa tattttatta
     4021 tagttatttt ccaaatttat ttgagatttt tgtattgaag tttagccgtc gacatggtct
     4081 tcacactcga agatttcgtt ggggactggc gacagacagc cggctacaac ctggaccaag
     4141 tccttgaaca gggaggtgtg tccagtttgt ttcagaatct cggggtgtcc gtaactccga
     4201 tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat cgacatccat gtcatcatcc
     4261 cgtatgaagg tctgagcggc gaccaaatgg gccagatcga aaaaattttt aaggtggtgt
     4321 accctgtgga tgatcatcac tttaaggtga tcctgcacta tggcacactg gtaatcgacg
     4381 gggttacgcc gaacatgatc gactatttcg gacggccgta tgaaggcatc gccgtgttcg
     4441 acggcaaaaa gatcactgta acagggaccc tgtggaacgg caacaaaatt atcgacgagc
     4501 gcctgatcaa ccccgacggc tccctgctgt tccgagtaac catcaacgga gtgaccggct
     4561 ggcggctgtg cgaacgcatt ctggcggcta gcggaagcgg agctactaac ttcagcctgc
     4621 tgaagcaggc tggagacgtg gaggagaacc ctggacctat gattgaacaa gatggtttac
     4681 acgctggttc tcccgccgct tgggtcgaaa gacttttcgg ttatgactgg gctcaacaaa
     4741 ccatcggttg ctctgatgcc gccgtcttcc gtctttctgc tcaaggtcgt cctgttcttt
     4801 tcgtcaagac cgacctttct ggtgccctta atgaacttca agatgaagct gcccgtcttt
     4861 cttggcttgc caccaccggt gttccttgcg ctgctgtcct tgacgttgtc actgaagccg
     4921 gtagagactg gcttctttta ggtgaagtcc ccggtcaaga tcttctttct tctcaccttg
     4981 ctcctgccga aaaagtttct atcatggctg atgctatgcg tcgtcttcat acccttgatc
     5041 ccgctacctg ccctttcgac caccaagcca aacatcgtat cgaacgtgct cgtactcgta
     5101 tggaagccgg tcttgtcgat caagatgatc ttgacgaaga acatcaaggt cttgcccctg
     5161 ccgaactttt cgccagactt aaggcccgta tgcccgacgg tgaagatctt gtcgtcaccc
     5221 atggtgatgc ctgcttaccc aatatcatgg ttgaaaatgg tcgtttttct ggtttcatcg
     5281 actgtggtcg tcttggtgtc gccgaccgtt atcaagatat tgccttagct acccgtgata
     5341 ttgctgaaga acttggtggt gaatgggctg accgtttcct tgtcctttac ggtatcgccg
     5401 ctcccgattc tcaacgtatc gccttctatc gtcttcttga cgaattcttc tgattaatta
     5461 aggcaaaatt tggcgcagct atggcgcctc ttgaaagtgc ctaaaaagga gtcaaagtta
     5521 gacagaccaa gaaaaggtaa caaggcaaga gaaaacatac attaatatat atacatatat
     5581 atatacttaa tagagatagt attactcttc tattctcttg tatagattgg tattagtagt
     5641 cttttatgga aaaggagaag agatgaataa aggttacaaa ggaggatgct tttctctaaa
     5701 agtaccacca gtgttttgca ataatgggaa tttattatta atcccaaaag gaagagataa
     5761 cgaaatttat gtatatgata ctttgggaaa tggagagtct aaatctcaat tttctgcttc
     5821 aaaagaaatt atcacaggtc ttggtgtgat aaagtctgat caaaatgagg aatttattgt
     5881 tactacaact gttagtggtt taatacgaat ttttcattta aatgatatct taaatcataa
     5941 agaaaagaag tctgtaatag agattgaaat tggtcgatgt attattgatt tgaaagttgc
     6001 aaaaaacaat atatatttta tcataagtga agcaaaagat caagatactt tgattgatac
     6061 taatgtttcc ttatataaaa ttcccatttc tgatataatt tcaaaattta atacttctgt
     6121 taatcaaaaa tctaatcatt ccaaatttat tcaaaaatct ctcaaaaaga ttattaactt
     6181 ctcatatgga gctttaaatt tccaagtatc ttcggatgag tctttattct gttttatatg
     6241 gaaaaatatc ttaattattt ggaatactca ataccctgat aaaattattc gttttagaca
     6301 ttccgaatat attctatcgc ttacactttc tgaagatcaa caatttgttg ctactggtga
     6361 tgcttatggt aggggtacca gctcggtacc cggggatcct ctagagtcga cctgcaggca
     6421 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6481 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6541 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6601 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6661 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6721 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6781 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6841 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7021 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7261 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7381 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7441 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7501 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7561 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7621 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7681 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7741 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7801 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7861 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7921 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7981 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8041 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8101 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8161 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8221 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8281 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8341 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8401 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8461 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8521 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8581 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     8641 gcgtatcacg aggccctttc gtc
//
