LOCUS       pET16b-Avi-ubiquitin_rbs_BirA        6984 bp    DNA     circular UNA 10-MAY-2018
DEFINITION  For production of biotinylated ubiquitin with PreSciscision
            cleavage site.
ACCESSION   urn.local...1478746486338.32
VERSION     urn.local...1478746486338.32
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Promoter        139..167
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     Marker          209..1069
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp marker"
                     /Transferred_Similarity="99.88%"
                     /label="amp marker"
     ORF             209..1069
                     /Genetic_code="Standard"
                     /Reading_frame=2
                     /created_by="ORF Finder"
                     /label="ORF 1 (frame 2)"
     misc_feature    1224..1843
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: Origin of Replication"
     Gene            complement(2258..2449)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    3448^3449
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    complement(3760..4851)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacI reg"
                     /note="Geneious type: Regulatory Sequence"
     ORF             complement(3760..4719)
                     /Genetic_code="Standard"
                     /Reading_frame=1
                     /created_by="ORF Finder"
                     /label="ORF 5 (frame 1)"
     Promoter        5229..5247
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    complement(5246..5273)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="82.14%"
                     /label="lacO reg"
                     /note="Geneious type: Regulatory Sequence"
     misc_feature    5247..5274
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: Regulatory Sequence"
     Gene            5292..5308
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     ORF             5316..5654
                     /Genetic_code="Standard"
                     /Reading_frame=1
                     /created_by="ORF Finder"
                     /modified_by="User"
                     /label="Avi-tag-Ubiquitin"
     misc_feature    5319..5351
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     Tag             5322..5351
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="6xHis tag"
                     /Transferred_Similarity="94.44%"
                     /modified_by="User"
                     /label="10xHis tag"
     domain          5352..5399
                     /created_by="User"
                     /modified_by="User"
                     /label="Biotinylation site"
     misc_feature    5353..6651
                     /Original_Bases="GCCATCATCATCATCATCATCATCATCATCACAGCAGCGGC
                     CATATCGAAGGTCGTCAT"
                     /label="GCCATCATCATCATCATCATCATCATCATCACAGCAGCGGCCATATCGAA
                     GGTCGTCAT"
                     /note="Geneious type: Editing History Replacement"
     Tag             5400..5420
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="precision tag"
                     /Transferred_Similarity="100.00%"
                     /label="precision tag"
     Gene            5666..5682
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     ORF             5690..6655
                     /Genetic_code="Standard"
                     /Reading_frame=2
                     /created_by="ORF Finder"
                     /label="ORF 2 (frame 2)"
     misc_feature    6655
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     Terminator      6730..6773
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
ORIGIN      
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctc tcgccgaaac gtttggtggc gggaccagtg
     3481 acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc
     3541 gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt
     3601 cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc
     3661 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt
     3721 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3781 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3841 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc
     3901 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag
     3961 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc
     4021 gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg
     4081 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc
     4141 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
     4201 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
     4261 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
     4321 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
     4381 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
     4441 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
     4501 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
     4561 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
     4621 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
     4681 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     4741 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
     4801 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg
     4861 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc
     4921 cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     4981 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
     5041 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     5101 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     5161 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
     5221 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
     5281 aataattttg tttaacttta agaaggagat ataccatggg ccatcatcat catcatcatc
     5341 atcatcatca cgtaggcctg aacgacatct tcgaggctca gaaaatcgaa tggcacgaac
     5401 tggaagttct gttccagggg cccatgcaaa tctttgttaa aaccctgacc ggtaaaacca
     5461 ttacactgga agttgaaccg agcgatacca ttgaaaatgt gaaagccaaa atccaggaca
     5521 aagaaggtat tccgcctgat cagcagcgtc tgatttttgc aggtaaacag ctggaagatg
     5581 gtcgtaccct gagcgattat aacattcaga aagaaagcac cctgcatctg gttctgcgtc
     5641 tgcgtggtgg ttgataaaat tttgtttaac tttaagaagg agatatacca tgaaggataa
     5701 cactgttccg ctgaaactga tcgccctgct ggccaatggc gagtttcata gcggtgaaca
     5761 gctgggtgaa actttaggca tgtcacgtgc tgctatcaac aagcatatcc agaccctgcg
     5821 tgattggggt gttgacgtat ttaccgtgcc gggtaaaggc tattccctgc cggaaccgat
     5881 tcagctgctg aacgcaaaac aaattctggg tcagctggac ggcggttccg tagctgtgct
     5941 gccggttatc gactctacta accagtacct cctggatcgc attggtgaac tgaaatccgg
     6001 ggacgcgtgc atcgcggagt atcagcaagc gggccgtggt cgtcgcggtc gtaaatggtt
     6061 ttcgccgttt ggtgcaaacc tgtacctcag catgttttgg cgtctggaac agggtccggc
     6121 agctgcaatc ggtttgtccc tggtaatcgg tatcgtaatg gcggaggttc tgcgtaaact
     6181 gggcgcagac aaagttcgtg tgaaatggcc gaacgacctt tacctgcaag atcgcaaact
     6241 ggctggtatc ctggttgagc tgaccggtaa aaccggggat gctgctcaga tcgttatcgg
     6301 tgcaggtatc aacatggcca tgcgtcgtgt agaggaaagc gtggttaacc agggttggat
     6361 taccctgcag gaggcgggca ttaacctgga ccgcaatact ctggcggcta tgctgatccg
     6421 cgaactgcgt gcggcactgg agctgttcga acaggaaggt ctggctccgt atcttagccg
     6481 ctgggaaaag ctcgataact tcatcaaccg tccggttaaa ctgattattg gtgataaaga
     6541 aatcttcggc atttcgcgcg gcatcgacaa acagggagcc ctgcttctgg aacaggatgg
     6601 tatcatcaaa ccgtggatgg gcggcgaaat ttctttacgc agcgctgaaa aatgactcga
     6661 ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     6721 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     6781 aggaactata tccggatatc ccgcaagagg cccggcagta ccggcataac caagcctatg
     6841 cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata
     6901 cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg
     6961 atgataagct gtcaaacatg agaa
//
