LOCUS       pUC19-Switch_repeat        3070 bp    DNA     circular     21-JAN-2021
DEFINITION  Cloning vector pUC19, complete sequence.
ACCESSION   urn.local...h-cx2g9u9
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New, England and Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA COMMENT See also GenBank
            accession L09137.
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Cloning vector pUC19"
                     /mol_type="other DNA"
     CDS             complement(146..853)
                     /gene="lacZalpha"
                     /codon_start=1
                     /product="beta-galactosidase alpha fragment"
                     /Original_Translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDW
                     ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISH
                     RIWCTLSTICSDAA"
     misc_feature    401^402
                     /Original_Bases="GAATTC"
                     /label="GAATTC"
                     /note="Geneious type: Editing History Deletion"
     Promoter        430..448
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
                     528..669
                     /created_by="adeans"
                     /label="SWITCH REPEAT"
     Terminator      782..825
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="93.18%"
                     /label="T7 term"
     misc_feature    830^831
                     /Original_Bases="AAGCTT"
                     /label="AAGCTT"
                     /note="Geneious type: Editing History Deletion"
     -10_signal      complement(898..903)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TATGTT"
     -35_signal      complement(922..927)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TTTACA"
     protein_bind    complement(947..959)
                     /note="CAP protein binding site"
                     /bound_moiety="catabolite activator protein (CAP)"
     rep_origin      complement(1251..1839)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     misc_RNA        complement(1251..1803)
                     /note="RNAII transcript (complementary strand)"
     -35_signal      1657..1662
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      1679..1684
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        1693..1800
                     /note="RNAI transcript"
     -10_signal      complement(1813..1818)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(1834..1839)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     gene            complement(2010..2870)
                     /gene="bla"
     CDS             complement(2010..2870)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     sig_peptide     complement(2802..2870)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
     -10_signal      complement(2914..2919)
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(2935..2940)
                     /note="bla promoter (counter-clockwise); TTCAAA"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgtaaaacga cggccagtga
      421 attgagttct aatacgactc actatagggc gaagaatgcc tcgagcttgc aatcgctagc
      481 tcaatatcct aactatcata cctatcacat aatcactcga cctagggggg tgctggggta
      541 ggttagagca tgggaaccag gctggacagc tctggaagga gctgagatat gtggggttgt
      601 ggggaacagg ttggacagct ctggggagct agggtagttg ggggtgtggg gactaggctg
      661 ggcagctcta ctagtgcttt caaccttggc gtaatcatgg tcatagctgt ttcctttcca
      721 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgt ctagagtcga
      781 ctgaacccct tggggcctct aaacgggtct tgaggggttt tttggtcgac aagcttggcg
      841 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      901 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      961 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     1021 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1081 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1141 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1201 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1261 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1321 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1381 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1441 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1501 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1561 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1621 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1681 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1741 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1801 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1861 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1921 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1981 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2041 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2101 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2161 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2221 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2281 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     2341 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2401 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2461 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2521 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2581 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2641 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2701 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2761 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2821 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2881 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2941 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3001 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     3061 ccctttcgtc
//
