LOCUS       pHRE-EV                10527 bp ds-DNA     circular     17-JUN-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     3'UTR               5648..5849
                         /label="3S0"
                         /ApEinfo_revcolor=#b1ff67
                         /ApEinfo_fwdcolor=#b1ff67
     promoter_eukaryotic 8816..8995
                         /label="nos"
                         /ApEinfo_revcolor=#85dae9
                         /ApEinfo_fwdcolor=#85dae9
     terminator          8020..8715
                         /label="35S terminator"
                         /ApEinfo_revcolor=#faac61
                         /ApEinfo_fwdcolor=#faac61
     CDS                 complement(1125..2606)
                         /label="TrfA"
                         /ApEinfo_revcolor=#f8d3a9
                         /ApEinfo_fwdcolor=#f8d3a9
     terminator          10232..10484
                         /label="nos"
                         /ApEinfo_revcolor=#b4abac
                         /ApEinfo_fwdcolor=#b4abac
     promoter_eukaryotic 5240..5554
                         /label="35S"
                         /ApEinfo_revcolor=#d6b295
                         /ApEinfo_fwdcolor=#d6b295
     misc_recomb         complement(4604..4765)
                         /label="RB"
                         /ApEinfo_revcolor=#faac61
                         /ApEinfo_fwdcolor=#faac61
     promoter_eukaryotic 6193..6592
                         /label="35S"
                         /ApEinfo_revcolor=#ffef86
                         /ApEinfo_fwdcolor=#ffef86
     CDS                 9040..10023
                         /label="NPTII"
                         /ApEinfo_revcolor=#faac61
                         /ApEinfo_fwdcolor=#faac61
     misc_recomb         complement(1..148)
                         /label="LB"
                         /ApEinfo_revcolor=#f58a5e
                         /ApEinfo_fwdcolor=#f58a5e
     rep_origin          complement(453..1070)
                         /label="OriV"
                         /ApEinfo_revcolor=#75c6a9
                         /ApEinfo_fwdcolor=#75c6a9
     rep_origin          3933..4522
                         /label="ColE1"
                         /ApEinfo_revcolor=#9eafd2
                         /ApEinfo_fwdcolor=#9eafd2
     enhancer            5070..5231
                         /label="35Se"
                         /ApEinfo_revcolor=#d59687
                         /ApEinfo_fwdcolor=#d59687
     5'UTR               5555..5614
                         /label="5S0"
                         /ApEinfo_revcolor=#b1ff67
                         /ApEinfo_fwdcolor=#b1ff67
     CDS                 6634..8013
                         /label="NSs"
                         /ApEinfo_revcolor=#d59687
                         /ApEinfo_fwdcolor=#d59687
     terminator          5850..6102
                         /label="nos"
                         /ApEinfo_revcolor=#ffef86
                         /ApEinfo_fwdcolor=#ffef86
     CDS                 complement(2607..3599)
                         /label="NPTIII"
                         /ApEinfo_revcolor=#f8d3a9
                         /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
       61 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
      121 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
      181 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
      241 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
      301 ttgctgcctg tgatcaaata tcatctccct cgcagagatc cgaattatca gccttcttat
      361 tcatttctcg cttaaccgtg acagagtaga caggctgtct cgcggccgag gggcgcagcc
      421 cctggggggg atgggaggcc cgcgttagcg ggccgggagg gttcgagaag ggggggcacc
      481 ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa caaggtttat
      541 aaatattggt ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa
      601 acccttgcaa atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc
      661 ccctcatctg tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt
      721 agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat
      781 ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca
      841 cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc
      901 ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta
      961 tccacaacgc cggcggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt
     1021 tgcagggcca tagacggccg ccagcccagc ggcgagggca accagcccgg tgagcgtcgg
     1081 aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtacac tagtcgctgg ctgctgaacc
     1141 cccagccgga actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac
     1201 ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc
     1261 gcgccacttc ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt
     1321 gagcgggtac ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga
     1381 cagcttgcgg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac
     1441 gatttcctcg tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg
     1501 gaagcggtgc agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat
     1561 cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat
     1621 cgaccagccc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg
     1681 ggtgcgcttc gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag
     1741 ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg
     1801 cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc
     1861 gtttggcatc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat
     1921 ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg
     1981 ttttgccagg tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc
     2041 gatggtcatc gacttcgcca aacctgccgc ctcctgttca agacgacgcg aacgctccac
     2101 ggcggccgat ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt
     2161 ggccgtagct tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat
     2221 gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc
     2281 ttgcctgtat gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc
     2341 gactcacgcc ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc
     2401 cagataatcc accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc
     2461 gtacttggta ttccgaatct tgccctgcac gaataccagc gaccccttgc ccaaatactt
     2521 gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg
     2581 cttgtcgccg gcatcgttgc gccacatcta ggtactaaaa caattcatcc agtaaaatat
     2641 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
     2701 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
     2761 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
     2821 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
     2881 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
     2941 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
     3001 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
     3061 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
     3121 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
     3181 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
     3241 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
     3301 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
     3361 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
     3421 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
     3481 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
     3541 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cacaattatg
     3601 ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc
     3661 ttctgtttct atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa
     3721 aagcaccgcc ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcaactgca
     3781 gttcacttac accgcttctc aacccggtac gcaccagaaa atcattgata tggccatgaa
     3841 tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt ggcctcaaca cgattttacg
     3901 tcacttaaaa aactcaggcc gcagtcggta actatgcggt gtgaaatacc gcacagatgc
     3961 gtaaggagaa aataccgcat caggcgctat tccgcttcct cgctcactga ctcgctgcgc
     4021 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4081 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4141 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4201 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4261 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4321 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4381 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4441 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4501 gacttatcgc cactggcagc aggtaacctc gcgcatacag ccgggcagtg acgtcatcgt
     4561 ctgcgcggaa atggacgggc ccccggcgcc agatctgggg aaccctgtgg ttggcatgca
     4621 catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa
     4681 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagtttg
     4741 tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     4801 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     4861 ggaagggaag aaagcgaaag gagcgggcgc cattcaggct gcgcaactgt tgggaagggc
     4921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     4981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     5041 aattgttaga gggagctctg atcactgagc atcgttgaag atgcctctgc cgacagtggt
     5101 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
     5161 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
     5221 cactatcctt cttaattaag gaaacctcct cggattccat tgcccagcta tctgtcactt
     5281 tattgagaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
     5341 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
     5401 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
     5461 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
     5521 ctctatataa ggaagttcat ttcatttgga gaggtttaag agacgcaacc acaacgctct
     5581 aacgcaatca atctacatta tattaaacgt ctctaaaaag agacctaggt aactgactag
     5641 ggtctctcgc cgaagagcgc atcggatcta ataataaact taggcaataa atttcgacat
     5701 cataataaag ccacgtatct acatcaacac aagattagta ttttcaaaac tgttttcagc
     5761 agaacacata ttttcatttt taacgtaatt ttcatttagc gttagtacag tcaccttcac
     5821 agaacgtttg gagaagtgag ggctcttccg atcgttcaaa catttggcaa taaagtttct
     5881 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     5941 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     6001 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     6061 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcggcgcgcc agcttggcgt
     6121 aatcatggtc atagctgttg cgattaataa ttccacgcgg atcgcattga gaacccggaa
     6181 tatcgcgaaa caggtacccc cctactccaa aaatgtcaaa gatacagtct cagaagacca
     6241 aagggctatt gagacttttc aacaaagggt aatttcggga aacctcctcg gattccattg
     6301 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
     6361 ccatcattgc gataaaggaa aggctatcat tcaagatgcc tctgccgaca gtggtcccaa
     6421 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     6481 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
     6541 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacagccca
     6601 agcttcgact ctagaggatc cccttaaatc gatatgagca ccgccaagat gagcgctggt
     6661 gagttctcta agacctacgg caccaaggat agccggtctg tgaatgattg ctacgccatc
     6721 tacagcggca acggcatcaa cttcatcaac ctgttcatgc acaccaacac cggcatcaag
     6781 agcgccttct ctattaacga ccttggccgg aacgaggaca ttaagcttca tgaggccgag
     6841 atcatcaaca gcttgcaccc gtactcattc ttcgacaagt tcggcctgga tatcatcctg
     6901 tgcaaccacg tgatggacat catcgtgact aagcctggcc ttaagaacac cggatgcaag
     6961 ttccagatgc acaaccagat cttcaacccg aatgaggata ttctggctaa gacccctggc
     7021 atcatcagcg aagagaactt ctacgacctg agcaagatca agcctaaggg catgacacct
     7081 ttcgactggt acatcaacga gtgcaagaag tacgacttct acatcagcga caacggcgac
     7141 atctctctgg attacggttt ccctgtgatg ggcaagacca cctcttattg gagagagaac
     7201 atcccgcgag agaagatcat ctccgttaag cagaagtgcc tgccgaacac ctctgctctt
     7261 accaacagga ttctgagcct ttctaccgtg agggctatcc agattgcttc tgagctggct
     7321 tctgaaaaga ccgtggttct tgcttctagg cagaggctgg atctggacat caagtctcag
     7381 taccggatct cattccctgg tgttcaagat gagggcgctt tcactaggac tttctgcatc
     7441 cctatggaca agaccgctag gatcgtttgc ttctacgcta agacctccgt ggacgtgtca
     7501 aatgagagga ccaccttgac catcaagatc gtcaacaaga ccgtcgagag caactacatg
     7561 ggtcctgtgc ctaaggatca catgtactgc gataagagca tcggcgctag gattggtctt
     7621 gtggatattg tgaggggcga cccgaactac aatcagatga ttgctcggga aatgatcagc
     7681 gtccacacca actttgctct gaggctttct gaggctctga agaagccggt catcgttttc
     7741 aagatgtacg agaaagagct gaacttcgag acttacgacc tgtccggtcg gtctttgtct
     7801 taccagaagg acagcagcgg caatatctac ttcctgtcta ggacccttga gatcctgccg
     7861 aagtctcttt ctaccctgac ctacctgaag aacatctctc ctgcctgctg gaaagaatcc
     7921 atcagcatgc agcatttcta catcggggag caagaagagg gaccttctgg ttctaatctg
     7981 gccgagtctg aagagaaggt gcagactgct taggaattcg gtacgctgaa atcaccagtc
     8041 tctctctaca aatctatctc tctctatttt ctccataaat aatgtgtgag tagtttcccg
     8101 ataagggaaa ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct
     8161 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
     8221 aaatccagta ctaaaatcca gatctcctaa agtccctata gatctttgtc gtgaatataa
     8281 accagacacc ggacgactaa acctggagcc cagacgccgt tcgaagctag aagtaccgct
     8341 taggcaggag gccgttaggg aaaagatgct aaggcagggt tggttacgtt gactcccccg
     8401 taggtttggt ttaaatatga tgaagtggac ggaaggaagg aggaagacaa ggaaggataa
     8461 ggttgcaggc cctgtgcaag gtaagaagat ggaaatttga tagaggtacg ctactatact
     8521 tatactatac gctaagggaa tgcttgtatt tataccctat accccctaat aaccccttat
     8581 caatttaaga aataatccgc ataagccccc gcttaaaaat tggtatcaga gccatgaata
     8641 ggtctatgac caaaactcaa gaggataaaa cctcaccaaa atacgaaaga gttcttaact
     8701 ctaaagataa aagatggcgc gtggccggcc tacagtatga gcggagaatt aagggagtca
     8761 cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc
     8821 gcaacgttga aggagccact cagccgcggg tttctggagt ttaatgagct aagcacatac
     8881 gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat
     8941 ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc
     9001 tcatattcac tctcaatcca aataatctgc accggatctg gatcgtttcg catgattgaa
     9061 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     9121 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     9181 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     9241 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     9301 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     9361 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     9421 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     9481 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agaacatcag
     9541 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgatgat
     9601 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     9661 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     9721 gctacccgtg atattgctga agaacttggc ggcgaatggg ctgaccgctt cctcgtgctt
     9781 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     9841 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     9901 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     9961 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacggga
    10021 tctctgcgga acaggcggtc gaaggtgccg atatcattac gacagcaacg gccgacaagc
    10081 acaacgccac gatcctgagc gacaatatga tcgcggcgtc cacatcaacg gcgtcggcgg
    10141 cgactgccca ggcaagaccg agatgcaccg cgatatcttg ctgcgttcgg atattttcgt
    10201 ggagttcccg ccacagaccc ggatgatccc cgatcgttca aacatttggc aataaagttt
    10261 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
    10321 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
    10381 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
    10441 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggact gtaggccggc
    10501 cctcactggt gaaaagaaaa accaccc
//