LOCUS Exported 5184 bp ds-DNA circular SYN 04-JUL-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5184) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Oct 11, 2019 from SnapGene Viewer 4.3.11 https://www.snapgene.com FEATURES Location/Qualifiers source 1..5184 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(135..441) /direction=LEFT /label=F1 ori CDS complement(460..528) /label=LacZ alpha primer_bind 599..616 /label=M13-fwd primer_bind 625..645 /label=T7 misc_feature 716..949 /label=U6 misc_feature 958..963 /label=BsmB1 /label=BsmB1(1) misc_feature 2831..2836 /label=BsmB1 misc_feature 2838..2919 /label=+85 ch RNA backbone primer_bind complement(2994..3013) /label=T3 primer_bind complement(3031..3051) /label=M13-rev misc_binding complement(3057..3079) /label=LacO rep_origin complement(3419..4101) /direction=LEFT /label=ColE1 origin CDS complement(4199..4858) /label=AmpR ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 661 ccgcggtggc ggccgctcta gaactagtgg atccgagggg acccagagag ggcctatttc 721 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attagaatta 781 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc 841 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa 901 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccggag 961 acggttgtaa atgagcacac aaaatacaca tgctaaaata ttatattcta tgacctttat 1021 aaaatcaacc aaaatcttct ttttaataac tttagtatca ataattagaa tttttatgtt 1081 cctttttgca aacttttaat aaaaatgagc aaaataaaaa aacgctagtt ttagtaactc 1141 gcgttgtttt cttcaccttt aataatagct actccaccac ttgttcctaa gcggtcagct 1201 cctgcttcaa tcattttttg agcatcttca aatgttctaa ctccaccagc tgctttaact 1261 aaagcattgt ctttaacaac tgacttcatt agtttaacat cttcaaatgt tgcacctgat 1321 tttgaaaatc ctgttgatgt tttaacaaat tctaatccag cttcaacagc tatttcacaa 1381 gctttcatga tttcttcttt tgttaataaa caattttcca taatacattt aacaacatgt 1441 gatccagctg ctttttttac agctttcatg tcttctaaaa ctaattcata atttttgtct 1501 tttaatgcac caatatttaa taccatatca atttctgttg caccatcttt aattgcttca 1561 gaaacttcga atgcttttgt agctgttgtg catgcaccta gaggaaaacc tacaacattt 1621 gttattccta catttgtgcc ttttaataat tctttacaat agcttgttca atatgaatta 1681 acacaaactg ttgcaaaatc aaattcaatt gcttcatcac ataattgttt aatttcagct 1741 ttcgtagcat cttgttttaa taatgtgtga tctatatatt tgtttagttt cattttttct 1801 cctatatatt catttttaat tttaattctt taataatttc gtctacttta actttagcgt 1861 tttgaacaga ttcaccaaca cctataaaat aaatttttag tttaggttca gttccacttg 1921 ggcgaacagc aaatcatgac ttatcttcta aataaaattt tagtaagtct tgtcctggca 1981 tattatacat tccatcgatg tagtcttcaa cattaacaac tttaagtcca gcaatttgag 2041 ttaagggtgt tgctctcaat gatttcatta atggttcaat ttttaatttc ttttcttctg 2101 gtttaaaatt caagtttaaa gtgaaagtgt aatatgcacc catttcttta aataaatctt 2161 ctaaatagtc tactaatgtt ttattttgtt ttttataaaa tcaagcagcc tctgctatta 2221 atatagaagc ttgtattcca tctttatctc tagctgagtc atcaattaca tatccataac 2281 tttcttcata agcaaaaaca aaatttaatc cgttatcttc ttctttagca atttctctac 2341 ccattcattt aaatccagtt aaagttttta caatattaac tccatatttt tcatgagcga 2401 ttctatcacc caaatcactt gttacaaaac ttgaatatag agccggattt tttggaatgc 2461 tatttaagcg ttttagattt gataattttc aatcaattaa aattggtcct gtttgatttc 2521 catctaatct tacaaaatga ccatcatgtt ttattgccat tccaaatctg tcagcatctg 2581 ggtcattcat aataataata tctgcatcat gtttaatacc atattcaagc ggtatttttc 2641 atgcaggatc aaattctgga tttggattta caacattttt aaatgtttca tcttcaaatg 2701 catgctcttc aacctcaata acgttatatc ctgattcacg taatattttt ggggtaaatt 2761 tagttcctgt tccattaact gcgctaaaaa taatttttaa atctttttta gcttcttgct 2821 cttttttgta cgtctctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt 2881 tatcaacttg aaaaagtggc accgagtcgg tgcttttttg aattctagat cttgagacaa 2941 atggcagtat tcatccacaa ttctcgaggg ggggcccggt acccagcttt tgttcccttt 3001 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 3061 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 3121 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 3181 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 3241 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3301 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 3361 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 3421 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 3481 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 3541 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 3601 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 3661 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 3721 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 3781 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 3841 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 3901 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 3961 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 4021 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 4081 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 4141 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 4201 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 4261 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 4321 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 4381 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 4441 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 4501 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 4561 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 4621 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 4681 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 4741 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 4801 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 4861 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 4921 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 4981 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 5041 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 5101 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 5161 gcacatttcc ccgaaaagtg ccac //