LOCUS       Exported                5184 bp ds-DNA     circular SYN 04-JUL-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 11, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(135..441)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(460..528)
                     /label=LacZ alpha
     primer_bind     599..616
                     /label=M13-fwd
     primer_bind     625..645
                     /label=T7
     misc_feature    716..949
                     /label=U6
     misc_feature    958..963
                     /label=BsmB1
                     /label=BsmB1(1)
     misc_feature    2831..2836
                     /label=BsmB1
     misc_feature    2838..2919
                     /label=+85 ch RNA backbone
     primer_bind     complement(2994..3013)
                     /label=T3
     primer_bind     complement(3031..3051)
                     /label=M13-rev
     misc_binding    complement(3057..3079)
                     /label=LacO
     rep_origin      complement(3419..4101)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(4199..4858)
                     /label=AmpR
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atccgagggg acccagagag ggcctatttc
      721 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attagaatta
      781 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc
      841 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa
      901 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccggag
      961 acggttgtaa atgagcacac aaaatacaca tgctaaaata ttatattcta tgacctttat
     1021 aaaatcaacc aaaatcttct ttttaataac tttagtatca ataattagaa tttttatgtt
     1081 cctttttgca aacttttaat aaaaatgagc aaaataaaaa aacgctagtt ttagtaactc
     1141 gcgttgtttt cttcaccttt aataatagct actccaccac ttgttcctaa gcggtcagct
     1201 cctgcttcaa tcattttttg agcatcttca aatgttctaa ctccaccagc tgctttaact
     1261 aaagcattgt ctttaacaac tgacttcatt agtttaacat cttcaaatgt tgcacctgat
     1321 tttgaaaatc ctgttgatgt tttaacaaat tctaatccag cttcaacagc tatttcacaa
     1381 gctttcatga tttcttcttt tgttaataaa caattttcca taatacattt aacaacatgt
     1441 gatccagctg ctttttttac agctttcatg tcttctaaaa ctaattcata atttttgtct
     1501 tttaatgcac caatatttaa taccatatca atttctgttg caccatcttt aattgcttca
     1561 gaaacttcga atgcttttgt agctgttgtg catgcaccta gaggaaaacc tacaacattt
     1621 gttattccta catttgtgcc ttttaataat tctttacaat agcttgttca atatgaatta
     1681 acacaaactg ttgcaaaatc aaattcaatt gcttcatcac ataattgttt aatttcagct
     1741 ttcgtagcat cttgttttaa taatgtgtga tctatatatt tgtttagttt cattttttct
     1801 cctatatatt catttttaat tttaattctt taataatttc gtctacttta actttagcgt
     1861 tttgaacaga ttcaccaaca cctataaaat aaatttttag tttaggttca gttccacttg
     1921 ggcgaacagc aaatcatgac ttatcttcta aataaaattt tagtaagtct tgtcctggca
     1981 tattatacat tccatcgatg tagtcttcaa cattaacaac tttaagtcca gcaatttgag
     2041 ttaagggtgt tgctctcaat gatttcatta atggttcaat ttttaatttc ttttcttctg
     2101 gtttaaaatt caagtttaaa gtgaaagtgt aatatgcacc catttcttta aataaatctt
     2161 ctaaatagtc tactaatgtt ttattttgtt ttttataaaa tcaagcagcc tctgctatta
     2221 atatagaagc ttgtattcca tctttatctc tagctgagtc atcaattaca tatccataac
     2281 tttcttcata agcaaaaaca aaatttaatc cgttatcttc ttctttagca atttctctac
     2341 ccattcattt aaatccagtt aaagttttta caatattaac tccatatttt tcatgagcga
     2401 ttctatcacc caaatcactt gttacaaaac ttgaatatag agccggattt tttggaatgc
     2461 tatttaagcg ttttagattt gataattttc aatcaattaa aattggtcct gtttgatttc
     2521 catctaatct tacaaaatga ccatcatgtt ttattgccat tccaaatctg tcagcatctg
     2581 ggtcattcat aataataata tctgcatcat gtttaatacc atattcaagc ggtatttttc
     2641 atgcaggatc aaattctgga tttggattta caacattttt aaatgtttca tcttcaaatg
     2701 catgctcttc aacctcaata acgttatatc ctgattcacg taatattttt ggggtaaatt
     2761 tagttcctgt tccattaact gcgctaaaaa taatttttaa atctttttta gcttcttgct
     2821 cttttttgta cgtctctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
     2881 tatcaacttg aaaaagtggc accgagtcgg tgcttttttg aattctagat cttgagacaa
     2941 atggcagtat tcatccacaa ttctcgaggg ggggcccggt acccagcttt tgttcccttt
     3001 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     3061 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3121 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3181 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3241 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3301 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3361 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3421 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3481 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3541 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3601 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3661 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3721 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3781 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3841 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3901 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3961 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4021 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4081 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4141 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4201 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4261 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4321 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4381 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4441 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4501 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4561 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4621 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4681 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4741 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4801 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4861 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4921 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4981 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5041 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5101 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5161 gcacatttcc ccgaaaagtg ccac
//
