LOCUS Exported 5194 bp ds-DNA circular SYN 04-JUL-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5194) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Oct 11, 2019 from SnapGene Viewer 4.3.11 https://www.snapgene.com COMMENT cassette retournee le 4 07 16 sac kpn1 FEATURES Location/Qualifiers source 1..5194 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(135..441) /direction=LEFT /label=F1 ori CDS complement(460..528) /label=LacZ alpha primer_bind 599..616 /label=M13-fwd primer_bind 625..645 /label=T7 misc_feature 716..949 /label=U6 misc_feature 958..963 /label=BsmB1 misc_feature 2831..2836 /label=BsmB1 /label=BsmB1(1) misc_feature join(2838..2841,2843..2849,2855..2858,2864..2872, 2874..2929) /label=+85 ch RNA backbone misc_feature 2842 /label=Stabilized_sgRNA_Mutation /label=Stabilized_sgRNA_Mutation(1) misc_feature 2850..2854 /label=Satbilized_sgRNA_Mutation misc_feature 2859..2863 /label=Stabilized_sgRNA_Mutation /label=Stabilized_sgRNA_Mutation(2) misc_feature 2873 /label=Stabilized_sgRNA_Mutation primer_bind complement(3004..3023) /label=T3 primer_bind complement(3041..3061) /label=M13-rev misc_binding complement(3067..3089) /label=LacO rep_origin complement(3429..4111) /direction=LEFT /label=ColE1 origin CDS complement(4209..4868) /label=AmpR ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 661 ccgcggtggc ggccgctcta gaactagtgg atccgagggg acccagagag ggcctatttc 721 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attagaatta 781 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc 841 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa 901 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccggag 961 acggttgtaa atgagcacac aaaatacaca tgctaaaata ttatattcta tgacctttat 1021 aaaatcaacc aaaatcttct ttttaataac tttagtatca ataattagaa tttttatgtt 1081 cctttttgca aacttttaat aaaaatgagc aaaataaaaa aacgctagtt ttagtaactc 1141 gcgttgtttt cttcaccttt aataatagct actccaccac ttgttcctaa gcggtcagct 1201 cctgcttcaa tcattttttg agcatcttca aatgttctaa ctccaccagc tgctttaact 1261 aaagcattgt ctttaacaac tgacttcatt agtttaacat cttcaaatgt tgcacctgat 1321 tttgaaaatc ctgttgatgt tttaacaaat tctaatccag cttcaacagc tatttcacaa 1381 gctttcatga tttcttcttt tgttaataaa caattttcca taatacattt aacaacatgt 1441 gatccagctg ctttttttac agctttcatg tcttctaaaa ctaattcata atttttgtct 1501 tttaatgcac caatatttaa taccatatca atttctgttg caccatcttt aattgcttca 1561 gaaacttcga atgcttttgt agctgttgtg catgcaccta gaggaaaacc tacaacattt 1621 gttattccta catttgtgcc ttttaataat tctttacaat agcttgttca atatgaatta 1681 acacaaactg ttgcaaaatc aaattcaatt gcttcatcac ataattgttt aatttcagct 1741 ttcgtagcat cttgttttaa taatgtgtga tctatatatt tgtttagttt cattttttct 1801 cctatatatt catttttaat tttaattctt taataatttc gtctacttta actttagcgt 1861 tttgaacaga ttcaccaaca cctataaaat aaatttttag tttaggttca gttccacttg 1921 ggcgaacagc aaatcatgac ttatcttcta aataaaattt tagtaagtct tgtcctggca 1981 tattatacat tccatcgatg tagtcttcaa cattaacaac tttaagtcca gcaatttgag 2041 ttaagggtgt tgctctcaat gatttcatta atggttcaat ttttaatttc ttttcttctg 2101 gtttaaaatt caagtttaaa gtgaaagtgt aatatgcacc catttcttta aataaatctt 2161 ctaaatagtc tactaatgtt ttattttgtt ttttataaaa tcaagcagcc tctgctatta 2221 atatagaagc ttgtattcca tctttatctc tagctgagtc atcaattaca tatccataac 2281 tttcttcata agcaaaaaca aaatttaatc cgttatcttc ttctttagca atttctctac 2341 ccattcattt aaatccagtt aaagttttta caatattaac tccatatttt tcatgagcga 2401 ttctatcacc caaatcactt gttacaaaac ttgaatatag agccggattt tttggaatgc 2461 tatttaagcg ttttagattt gataattttc aatcaattaa aattggtcct gtttgatttc 2521 catctaatct tacaaaatga ccatcatgtt ttattgccat tccaaatctg tcagcatctg 2581 ggtcattcat aataataata tctgcatcat gtttaatacc atattcaagc ggtatttttc 2641 atgcaggatc aaattctgga tttggattta caacattttt aaatgtttca tcttcaaatg 2701 catgctcttc aacctcaata acgttatatc ctgattcacg taatattttt ggggtaaatt 2761 tagttcctgt tccattaact gcgctaaaaa taatttttaa atctttttta gcttcttgct 2821 cttttttgta cgtctctgtt taagagctat gctggaaaca gcatagcaag tttaaataag 2881 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg aattctagat 2941 cttgagacaa atggcagtat tcatccacaa ttctcgaggg ggggcccggt acccagcttt 3001 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct 3061 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 3121 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 3181 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 3241 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 3301 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 3361 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 3421 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 3481 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 3541 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 3601 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 3661 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 3721 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 3781 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 3841 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 3901 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 3961 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 4021 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 4081 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 4141 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 4201 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 4261 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 4321 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 4381 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 4441 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 4501 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 4561 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 4621 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 4681 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 4741 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 4801 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 4861 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 4921 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 4981 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 5041 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 5101 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 5161 ggggttccgc gcacatttcc ccgaaaagtg ccac //